safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
8	0	0	8.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
AOGINLDG_4	157611	158816	Mef(En2)	ARO:3004659	Mef(En2)	99.25	100.00	AF251288.1
AOGINLDG_5	100401	101480	tet(X1)	ARO:3005166	tet(X1)	99.72	100.00	AJ311171.1
AOGINLDG_5	99066	100142	tet(X4)	ARO:3004720	tet(X4)	95.81	92.99	MK134376.1
AOGINLDG_5	98027	98890	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
AOGINLDG_5	14469	16442	tet(Q)	ARO:3000191	tet(Q)	96.04	100.00	Z21523.1
AOGINLDG_3	1245686	1247038	tet(Q)	ARO:3000191	tet(Q)	96.37	68.49	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
AOGINLDG_5	99066	100232	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.74	100.00	M37699
AOGINLDG_5	14517	16442	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.69	100.00	L33696
AOGINLDG_5	24422	25219	aph(3')-III	Kanamycin, Amikacin, Neomycin, Butirosin, Isepamicin, Lividomycin, Paromomycin, Ribostamycin	99.37	100.00	M26832
AOGINLDG_5	1	504	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.0	62.92	M62487

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
AOGINLDG_5	98030	98890	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
AOGINLDG_4	157077	157586	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
AOGINLDG_5	100404	101540	tet(X1)	tetracycline-inactivating monooxygenase Tet(X1)	BLASTX	99.74	100.00	WP_204376222.1
AOGINLDG_5	99069	100232	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	BLASTX	99.74	100.00	WP_008651082.1
AOGINLDG_4	157614	158816	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.25	100.00	WP_063853729.1
AOGINLDG_5	14517	16439	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	99.22	100.00	WP_063856407.1
AOGINLDG_4	2411563	2412222	cblA	CblA family class A beta-lactamase	PARTIALX	98.18	74.32	WP_005827792.1
AOGINLDG_5	24422	25216	aph(3')-IIIa	aminoglycoside O-phosphotransferase APH(3')-IIIa	INTERNAL_STOP	94.34	100.00	WP_001096887.1
AOGINLDG_5	1	501	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	PARTIAL_CONTIG_ENDX	100.00	62.78	WP_063844771.1
AOGINLDG_3	1245716	1247038	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	PARTIALX	99.32	68.80	WP_063856407.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AOGINLDG_1	1.0	0.0	1.0
AOGINLDG_2	1.0	0.0	1.0
AOGINLDG_3	0.0	1.0	0.0
AOGINLDG_4	0.0	1.0	0.0
AOGINLDG_5	0.0	1.0	0.0
AOGINLDG_6	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
AOGINLDG_3	1346823	1351284	False	Podoviridae	VOG4567,
AOGINLDG_6	52493	65388	False	Unknown	VOG0083,

Phigaro v2.4.0 (Database: Jan. 2024 release)