safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
18	0	0	18.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
HBOGDHGI_5	1362706	1363569	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
HBOGDHGI_5	1360333	1361463	ErmF	ARO:3000498	ErmF	99.62	141.35	M17124.1
HBOGDHGI_5	1259716	1260681	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_5	792696	793586	CblA-1	ARO:3002999	CblA-1	100.00	100.00	GQ343019.1
HBOGDHGI_2	459503	460468	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_4	445132	447057	tet(Q)	ARO:3000191	tet(Q)	96.88	97.56	Z21523.1
HBOGDHGI_16	347428	348360	CfxA3	ARO:3003003	CfxA3	99.68	96.57	AF472622.2
HBOGDHGI_4	343863	344828	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_5	280040	281005	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_6	232614	233579	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_6	222545	223477	CfxA3	ARO:3003003	CfxA3	99.68	96.57	AF472622.2
HBOGDHGI_6	178709	179641	CfxA3	ARO:3003003	CfxA3	99.68	96.57	AF472622.2
HBOGDHGI_13	99582	100547	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_5	90573	91538	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_11	80931	81896	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_4	31959	32924	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2
HBOGDHGI_2	18556	19521	CfxA3	ARO:3003003	CfxA3	100.00	100.00	AF472622.2

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
HBOGDHGI_11	80931	81896	cfxA3	Ampicillin	100.00	100.00	AF472622
HBOGDHGI_5	1360663	1361463	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	M17808
HBOGDHGI_4	445132	447057	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.95	100.00	X58717

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
HBOGDHGI_5	1362709	1363569	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
HBOGDHGI_5	792699	793586	cblA	CblA family class A beta-lactamase	EXACTX	100.00	100.00	WP_005827792.1
HBOGDHGI_11	80931	81893	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_13	99585	100547	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_2	18556	19518	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_2	459503	460465	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_4	31962	32924	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_4	343866	344828	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_5	1259716	1260678	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_5	280043	281005	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_5	90576	91538	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_6	232617	233579	cfxA3	extended-spectrum class A beta-lactamase CfxA3	EXACTX	100.00	100.00	WP_004348227.1
HBOGDHGI_5	1360663	1361460	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
HBOGDHGI_4	445132	447054	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	99.84	100.00	WP_004293868.1
HBOGDHGI_16	347398	348357	cfxA3	extended-spectrum class A beta-lactamase CfxA3	BLASTX	99.06	99.69	WP_004348227.1
HBOGDHGI_6	178679	179638	cfxA3	extended-spectrum class A beta-lactamase CfxA3	BLASTX	99.06	99.69	WP_004348227.1
HBOGDHGI_6	222548	223507	cfxA3	extended-spectrum class A beta-lactamase CfxA3	BLASTX	99.06	99.69	WP_004348227.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HBOGDHGI_1	1.0	0.0	1.0
HBOGDHGI_2	0.0	1.0	0.0
HBOGDHGI_3	0.0	1.0	0.0
HBOGDHGI_4	0.0	1.0	0.0
HBOGDHGI_5	0.0	1.0	0.0
HBOGDHGI_6	0.0	1.0	0.0
HBOGDHGI_7	0.0	1.0	0.0
HBOGDHGI_8	0.0	1.0	0.0
HBOGDHGI_9	0.0	1.0	0.0
HBOGDHGI_10	1.0	0.0	1.0
HBOGDHGI_11	0.0	1.0	0.0
HBOGDHGI_12	0.0	1.0	0.0
HBOGDHGI_13	0.0	1.0	0.0
HBOGDHGI_14	0.0	1.0	0.0
HBOGDHGI_15	0.0	1.0	0.0
HBOGDHGI_16	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found