safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
15	0	0	15.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
DMCNLPFK_4	3002442	3004367	tet(Q)	ARO:3000191	tet(Q)	97.35	97.56	Z21523.1
DMCNLPFK_3	1762907	1763872	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
DMCNLPFK_3	1490372	1491337	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
DMCNLPFK_3	1430298	1431263	CfxA2	ARO:3003002	CfxA2	99.38	100.00	AF118110.1
DMCNLPFK_3	1216033	1216998	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
DMCNLPFK_4	865380	866345	CfxA2	ARO:3003002	CfxA2	99.38	100.00	AF118110.1
DMCNLPFK_3	783120	785066	tet(Q)	ARO:3000191	tet(Q)	97.49	98.63	Z21523.1
DMCNLPFK_4	125947	126762	sul2	ARO:3000412	sul2	100.00	100.00	AY055428.1
DMCNLPFK_4	125083	125886	APH(3'')-Ib	ARO:3002639	APH(3'')-Ib	99.63	100.00	AF313472.2
DMCNLPFK_4	124247	125083	APH(6)-Id	ARO:3002660	APH(6)-Id	99.64	100.00	AF024602.1
DMCNLPFK_4	121533	123507	tet(Q)	ARO:3000191	tet(Q)	100.00	97.56	Z21523.1
DMCNLPFK_3	91881	92744	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
DMCNLPFK_3	89818	90639	ErmF	ARO:3000498	ErmF	99.62	102.63	M17124.1
DMCNLPFK_3	22647	23612	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
DMCNLPFK_2	2891	3238	nimE	ARO:3007107	nimE	100.00	67.65	CP036548.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
DMCNLPFK_4	125083	125886	aph(3'')-Ib	Streptomycin	100.00	100.00	AF321551
DMCNLPFK_4	124247	125083	aph(6)-Id	Streptomycin	100.00	100.00	M28829
DMCNLPFK_4	125947	126762	sul2	Sulfamethoxazole	100.00	100.00	AY034138
DMCNLPFK_4	121533	123458	tet(Q)	Doxycycline, Tetracycline, Minocycline	100.00	100.00	Z21523
DMCNLPFK_3	1216033	1216998	cfxA5	Unknown Beta-lactam	99.90	100.00	AY769934
DMCNLPFK_3	89839	90639	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.88	100.00	M17808
DMCNLPFK_3	1430298	1431263	cfxA3	Ampicillin	99.69	100.00	AF472622
DMCNLPFK_3	1430298	1431263	cfxA4	Unknown Beta-lactam	99.69	100.00	AY769933
DMCNLPFK_3	1430298	1431263	cfxA	Cefoxitin, Ampicillin	99.69	100.00	U38243
DMCNLPFK_2	2734	3238	nimE	Metronidazole	98.44	98.44	AM042593

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
DMCNLPFK_3	91884	92744	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
DMCNLPFK_4	125086	125886	aph(3'')-Ib	aminoglycoside O-phosphotransferase APH(3'')-Ib	EXACTX	100.00	100.00	WP_001082319.1
DMCNLPFK_4	124250	125083	aph(6)-Id	aminoglycoside O-phosphotransferase APH(6)-Id	EXACTX	100.00	100.00	WP_000480968.1
DMCNLPFK_3	89839	90636	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
DMCNLPFK_4	125950	126762	sul2	sulfonamide-resistant dihydropteroate synthase Sul2	EXACTX	100.00	100.00	WP_001043260.1
DMCNLPFK_4	121536	123458	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	EXACTX	100.00	100.00	WP_002560998.1
DMCNLPFK_3	1216033	1216995	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
DMCNLPFK_3	1490375	1491337	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
DMCNLPFK_3	1762907	1763869	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
DMCNLPFK_3	22647	23609	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
DMCNLPFK_3	1430301	1431263	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.38	100.00	WP_004339683.1
DMCNLPFK_4	865380	866342	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.38	100.00	WP_004339683.1
DMCNLPFK_3	783153	785066	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.65	99.53	WP_063856407.1
DMCNLPFK_4	3002442	3004364	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.35	100.00	WP_002560998.1
DMCNLPFK_2	2915	3238	nimE	5-nitroimidazole reductase NimE	PARTIALX	100.00	63.53	WP_005812773.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DMCNLPFK_1	1.0	0.0	1.0
DMCNLPFK_2	1.0	0.0	1.0
DMCNLPFK_3	0.0	1.0	0.0
DMCNLPFK_4	0.0	1.0	0.0
DMCNLPFK_5	0.0	1.0	0.0
DMCNLPFK_6	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
DMCNLPFK_4	1680716	1687369	False	Siphoviridae	VOG0204,