safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
8	0	0	8.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
EAMMJKIM_5	4035986	4036951	CfxA2	ARO:3003002	CfxA2	100.00	100.00	AF118110.1
EAMMJKIM_5	1402483	1404369	tet(Q)	ARO:3000191	tet(Q)	96.97	95.59	Z21523.1
EAMMJKIM_5	1385205	1386005	ErmF	ARO:3000498	ErmF	97.74	100.00	M17124.1
EAMMJKIM_5	1383760	1384899	tet(X1)	ARO:3005166	tet(X1)	99.72	105.57	AJ311171.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
EAMMJKIM_5	1402483	1404409	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.95	100.00	X58717
EAMMJKIM_5	4035986	4036951	cfxA3	Ampicillin	99.90	100.00	AF472622
EAMMJKIM_5	4035986	4036951	cfxA4	Unknown Beta-lactam	99.90	100.00	AY769933
EAMMJKIM_5	4035986	4036951	cfxA5	Unknown Beta-lactam	99.90	100.00	AY769934
EAMMJKIM_5	4035986	4036951	cfxA	Cefoxitin, Ampicillin	99.90	100.00	U38243
EAMMJKIM_5	1385205	1386005	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.88	100.00	M62487
EAMMJKIM_5	1382424	1383591	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.74	100.00	M37699

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
EAMMJKIM_5	211905	212810	blaMUN-1	MUN family extended-spectrum class A beta-lactamase MUN-1	ALLELEX	100.00	100.00	WP_206340447.1
EAMMJKIM_5	4035986	4036948	cfxA	CfxA family broad-spectrum class A beta-lactamase	EXACTX	100.00	100.00	WP_004339683.1
EAMMJKIM_5	1402483	1404363	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	100.00	97.82	WP_004293868.1
EAMMJKIM_5	1383763	1384899	tet(X1)	tetracycline-inactivating monooxygenase Tet(X1)	BLASTX	99.74	100.00	WP_204376222.1
EAMMJKIM_5	1385205	1386002	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	BLASTX	99.62	100.00	WP_063844771.1
EAMMJKIM_5	1381387	1382115	aadS	aminoglycoside 6-adenylyltransferase AadS	PARTIALX	98.35	84.67	WP_003013318.1
EAMMJKIM_5	3929109	3930062	cfxA5	broad-spectrum class A beta-lactamase CfxA5	BLASTX	83.65	99.07	WP_032946415.1
EAMMJKIM_5	1382427	1383176	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	PARTIALX	98.40	64.43	WP_008651082.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EAMMJKIM_1	1.0	0.0	1.0
EAMMJKIM_2	0.0	1.0	0.0
EAMMJKIM_3	0.0	1.0	0.0
EAMMJKIM_4	1.0	0.0	1.0
EAMMJKIM_5	0.0	1.0	0.0
EAMMJKIM_6	0.0	1.0	0.0
EAMMJKIM_7	0.0	1.0	0.0
EAMMJKIM_8	0.0	1.0	0.0
EAMMJKIM_9	1.0	0.0	1.0
EAMMJKIM_10	0.0	1.0	0.0
EAMMJKIM_11	1.0	0.0	1.0
EAMMJKIM_12	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
EAMMJKIM_5	2610535	2618830	False	Siphoviridae	VOG4841,

Phigaro v2.4.0 (Database: Jan. 2024 release)