Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 0 | 0 | 10.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKJCCHOH_4 | 5544014 | 5544877 | aadS | ARO:3004683 | aadS | 100.00 | 100.00 | M72415.1 |
| NKJCCHOH_4 | 1690717 | 1692642 | tet(Q) | ARO:3000191 | tet(Q) | 96.26 | 97.56 | Z21523.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NKJCCHOH_4 | 5542109 | 5542772 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 82.90 | M17808 |
| NKJCCHOH_4 | 1690717 | 1692642 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.79 | 100.00 | L33696 |
| NKJCCHOH_4 | 7075712 | 7076526 | sul2 | Sulfamethoxazole | 99.75 | 99.88 | AY034138 |
| NKJCCHOH_4 | 169163 | 169895 | erm(G) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.18 | 99.73 | M15332 |
| NKJCCHOH_4 | 166928 | 168391 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 97.54 | 100.00 | AF274302 |
| NKJCCHOH_4 | 165588 | 166802 | mef(A) | Erythromycin, Azithromycin | 94.24 | 99.75 | AF227520 |
| NKJCCHOH_4 | 3628076 | 3629026 | cfxA3 | Ampicillin | 86.01 | 98.34 | AF472622 |
| NKJCCHOH_4 | 3628076 | 3629026 | cfxA4 | Unknown Beta-lactam | 86.01 | 98.34 | AY769933 |
| NKJCCHOH_4 | 3628076 | 3629026 | cfxA5 | Unknown Beta-lactam | 86.01 | 98.34 | AY769934 |
| NKJCCHOH_4 | 3628076 | 3629026 | cfxA | Cefoxitin, Ampicillin | 86.01 | 98.34 | U38243 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKJCCHOH_4 | 5544017 | 5544877 | aadS | aminoglycoside 6-adenylyltransferase AadS | EXACTX | 100.00 | 100.00 | WP_003013318.1 |
| NKJCCHOH_4 | 5542110 | 5542769 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | PARTIALX | 100.00 | 82.71 | WP_002682030.1 |
| NKJCCHOH_4 | 1690717 | 1692639 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.38 | 100.00 | WP_063856407.1 |
| NKJCCHOH_4 | 7075843 | 7076526 | sul2 | sulfonamide-resistant dihydropteroate synthase Sul2 | PARTIALX | 99.12 | 84.13 | WP_001043260.1 |
| NKJCCHOH_4 | 166928 | 168388 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | BLASTX | 97.13 | 100.00 | WP_000420313.1 |
| NKJCCHOH_4 | 165588 | 166811 | mef(A) | macrolide efflux MFS transporter Mef(A) | BLASTX | 96.57 | 100.00 | WP_012102963.1 |
| NKJCCHOH_4 | 3628074 | 3629027 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 83.65 | 99.07 | WP_004339683.1 |
| NKJCCHOH_4 | 3732622 | 3733575 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 83.65 | 99.07 | WP_004339683.1 |
| NKJCCHOH_4 | 7077627 | 7078580 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 83.65 | 99.07 | WP_004339683.1 |
| NKJCCHOH_4 | 169163 | 169639 | erm(G) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G) | PARTIALX | 100.00 | 65.16 | WP_063844787.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| NKJCCHOH_2 | 1752 | 2448 | repUS2 | 699 / 681 | 94.99 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NKJCCHOH_1 | 1.0 | 0.0 | 1.0 |
| NKJCCHOH_2 | 1.0 | 0.0 | 1.0 |
| NKJCCHOH_3 | 1.0 | 0.0 | 1.0 |
| NKJCCHOH_4 | 0.0 | 1.0 | 0.0 |
| NKJCCHOH_5 | 1.0 | 0.0 | 1.0 |
| NKJCCHOH_6 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NKJCCHOH_4 | 4334224 | 4337513 | False | Unknown | VOG8909, |
| NKJCCHOH_4 | 5063042 | 5069629 | False | Unknown | VOG0025, |
Phigaro v2.4.0 (Database: Jan. 2024 release)