Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 0 | 0 | 13.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LEPPKJKJ_5 | 1600803 | 1602728 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| LEPPKJKJ_5 | 1579199 | 1581124 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| LEPPKJKJ_5 | 1409262 | 1410062 | ErmF | ARO:3000498 | ErmF | 98.50 | 100.00 | M17124.1 |
| LEPPKJKJ_5 | 1407050 | 1408975 | tet(Q) | ARO:3000191 | tet(Q) | 89.08 | 97.56 | Z21523.1 |
| LEPPKJKJ_5 | 736920 | 737885 | CfxA5 | ARO:3003096 | CfxA5 | 99.38 | 100.00 | AY769934.1 |
| LEPPKJKJ_5 | 432411 | 434357 | tet(Q) | ARO:3000191 | tet(Q) | 97.81 | 98.63 | Z21523.1 |
| LEPPKJKJ_4 | 115291 | 116427 | tet(X) | ARO:3000205 | tet(X) | 99.21 | 97.42 | M37699.1 |
| LEPPKJKJ_4 | 113953 | 115092 | tet(X1) | ARO:3005166 | tet(X1) | 99.72 | 105.57 | AJ311171.1 |
| LEPPKJKJ_4 | 111246 | 112046 | ErmF | ARO:3000498 | ErmF | 97.74 | 100.00 | M17124.1 |
| LEPPKJKJ_4 | 95320 | 97245 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| LEPPKJKJ_4 | 73808 | 75733 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LEPPKJKJ_4 | 111246 | 112046 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.88 | 100.00 | M62487 |
| LEPPKJKJ_5 | 1610706 | 1611501 | aph(3')-III | Kanamycin, Amikacin, Neomycin, Butirosin, Isepamicin, Lividomycin, Paromomycin, Ribostamycin | 99.87 | 100.00 | M26832 |
| LEPPKJKJ_4 | 73808 | 75733 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84423676012462 | 100.00 | L33696 |
| LEPPKJKJ_4 | 115261 | 116427 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.74 | 100.00 | M37699 |
| LEPPKJKJ_5 | 736920 | 737885 | cfxA5 | Unknown Beta-lactam | 98.65 | 100.00 | AY769934 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LEPPKJKJ_5 | 1610737 | 1611498 | aph(3')-IIIa | aminoglycoside O-phosphotransferase APH(3')-IIIa | BLASTX | 100.00 | 96.21 | WP_001096887.1 |
| LEPPKJKJ_4 | 113953 | 115089 | tet(X1) | tetracycline-inactivating monooxygenase Tet(X1) | BLASTX | 99.74 | 100.00 | WP_204376222.1 |
| LEPPKJKJ_4 | 115261 | 116424 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | BLASTX | 99.74 | 100.00 | WP_008651082.1 |
| LEPPKJKJ_4 | 111249 | 112046 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 99.62 | 100.00 | WP_063844771.1 |
| LEPPKJKJ_4 | 73811 | 75733 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| LEPPKJKJ_5 | 1579199 | 1581121 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| LEPPKJKJ_5 | 736920 | 737882 | cfxA5 | broad-spectrum class A beta-lactamase CfxA5 | BLASTX | 99.38 | 100.00 | WP_032946415.1 |
| LEPPKJKJ_4 | 95323 | 97245 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 98.91 | 100.00 | WP_063856407.1 |
| LEPPKJKJ_5 | 1600803 | 1602725 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 98.91 | 100.00 | WP_063856407.1 |
| LEPPKJKJ_5 | 1409265 | 1410062 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 98.87 | 100.00 | WP_002682030.1 |
| LEPPKJKJ_5 | 432411 | 434324 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 97.96 | 99.53 | WP_063856407.1 |
| LEPPKJKJ_4 | 116603 | 117316 | aadS | aminoglycoside 6-adenylyltransferase AadS | PARTIALX | 94.96 | 82.58 | WP_003013318.1 |
| LEPPKJKJ_5 | 1407053 | 1408975 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 89.55 | 100.00 | WP_063856407.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LEPPKJKJ_1 | 1.0 | 0.0 | 1.0 |
| LEPPKJKJ_2 | 1.0 | 0.0 | 1.0 |
| LEPPKJKJ_3 | 1.0 | 0.0 | 1.0 |
| LEPPKJKJ_4 | 0.0 | 1.0 | 0.0 |
| LEPPKJKJ_5 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)