safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
12	0	0	12.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
HOHIHFAP_5	3883075	3883812	ErmB	ARO:3000375	ErmB	97.96	98.79	AF242872.1
HOHIHFAP_5	1838110	1839315	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
HOHIHFAP_4	1539748	1540887	tet(X1)	ARO:3005166	tet(X1)	99.72	105.57	AJ311171.1
HOHIHFAP_4	1537373	1538236	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
HOHIHFAP_4	1558431	1559810	tet(Q)	ARO:3000191	tet(Q)	97.06	69.86	Z21523.1
HOHIHFAP_5	1470887	1472185	tet(Q)	ARO:3000191	tet(Q)	96.99	65.75	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
HOHIHFAP_4	1543068	1543806	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	92.26	M62487
HOHIHFAP_5	3883075	3883812	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.73	100.00	U18931
HOHIHFAP_5	1470258	1472185	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.69	100.00	X58717
HOHIHFAP_4	1538412	1539579	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.40	100.00	M37699

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
HOHIHFAP_5	3878503	3879276	aad9	ANT(9) family aminoglycoside nucleotidyltransferase	EXACTX	100.00	100.00	WP_002578722.1
HOHIHFAP_4	1537376	1538236	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
HOHIHFAP_5	2813284	2814189	blaMUN-1	MUN family extended-spectrum class A beta-lactamase MUN-1	ALLELEX	100.00	100.00	WP_206340447.1
HOHIHFAP_5	1839340	1839849	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
HOHIHFAP_5	1470659	1472182	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	INTERNAL_STOP	99.80	79.25	WP_004293868.1
HOHIHFAP_4	1539751	1540887	tet(X1)	tetracycline-inactivating monooxygenase Tet(X1)	BLASTX	99.74	100.00	WP_204376222.1
HOHIHFAP_5	1838110	1839312	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
HOHIHFAP_5	3883075	3883809	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.18	100.00	WP_002292226.1
HOHIHFAP_4	1543027	1543803	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	BLASTX	95.79	98.12	WP_063844771.1
HOHIHFAP_4	1557882	1559807	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	INTERNAL_STOP	87.93	100.00	WP_063856407.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HOHIHFAP_1	1.0	0.0	1.0
HOHIHFAP_2	1.0	0.0	1.0
HOHIHFAP_3	0.0	1.0	0.0
HOHIHFAP_4	0.0	1.0	0.0
HOHIHFAP_5	0.0	1.0	0.0
HOHIHFAP_6	0.0	1.0	0.0
HOHIHFAP_7	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
HOHIHFAP_5	1014997	1023456	True	Unknown	VOG8253,

Phigaro v2.4.0 (Database: Jan. 2024 release)