Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 0 | 0 | 5.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MILKMIMF_11 | 2858658 | 2859653 | CfxA6 | ARO:3003097 | CfxA6 | 98.79 | 100.00 | GQ342996.1 |
| MILKMIMF_11 | 614744 | 616718 | tet(Q) | ARO:3000191 | tet(Q) | 100.00 | 97.56 | Z21523.1 |
| MILKMIMF_13 | 309983 | 311122 | tet(X1) | ARO:3005166 | tet(X1) | 99.72 | 105.57 | AJ311171.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MILKMIMF_11 | 614744 | 616669 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 100.00 | 100.00 | Z21523 |
| MILKMIMF_13 | 308647 | 309814 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.74 | 100.00 | M37699 |
| MILKMIMF_11 | 2858658 | 2859653 | cfxA6 | Unknown Beta-lactam | 99.40 | 99.90 | GQ342996 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MILKMIMF_11 | 614747 | 616669 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | EXACTX | 100.00 | 100.00 | WP_002560998.1 |
| MILKMIMF_13 | 309986 | 311122 | tet(X1) | tetracycline-inactivating monooxygenase Tet(X1) | BLASTX | 99.74 | 100.00 | WP_204376222.1 |
| MILKMIMF_11 | 2858691 | 2859650 | cfxA6 | broad-spectrum class A beta-lactamase CfxA6 | BLASTX | 99.06 | 100.00 | WP_117664257.1 |
| MILKMIMF_13 | 307646 | 308338 | aadS | aminoglycoside 6-adenylyltransferase AadS | PARTIALX | 98.27 | 80.49 | WP_003013318.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MILKMIMF_1 | 864 | 1544 | repUS2 | 683 / 681 | 98.24 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MILKMIMF_1 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_2 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_3 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_4 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_5 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_6 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_7 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_8 | 1.0 | 0.0 | 1.0 |
| MILKMIMF_9 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_10 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_11 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_12 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_13 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_14 | 0.0 | 1.0 | 0.0 |
| MILKMIMF_15 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MILKMIMF_9 | 7692 | 13324 | False | Unknown | VOG4652, |
Phigaro v2.4.0 (Database: Jan. 2024 release)