safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
10	0	0	10.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
NAFPDFEE_1	5725419	5727305	tet(Q)	ARO:3000191	tet(Q)	96.97	95.59	Z21523.1
NAFPDFEE_1	4831982	4832845	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
NAFPDFEE_1	4828574	4829704	ErmF	ARO:3000498	ErmF	99.62	141.35	M17124.1
NAFPDFEE_1	3227093	3227827	ErmG	ARO:3000522	ErmG	99.18	100.00	L42817.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
NAFPDFEE_1	4828904	4829704	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	M17808
NAFPDFEE_1	5725419	5727345	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.95	100.00	X58717
NAFPDFEE_1	1223315	1224282	cfxA3	Ampicillin	99.79	100.00	AF472622
NAFPDFEE_1	3227093	3227827	erm(G)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.32	100.00	M15332
NAFPDFEE_1	3224857	3226321	msr(D)	Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S	97.47	100.00	AF274302
NAFPDFEE_1	3223577	3224731	mef(A)	Erythromycin, Azithromycin	93.51	94.66	AF227520

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
NAFPDFEE_1	4831985	4832845	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
NAFPDFEE_1	4828904	4829701	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
NAFPDFEE_1	5725419	5727299	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	100.00	97.82	WP_004293868.1
NAFPDFEE_1	1223482	1224282	cfxA3	extended-spectrum class A beta-lactamase CfxA3	PARTIALX	100.00	83.18	WP_004348227.1
NAFPDFEE_1	3947511	3948311	cfxA3	extended-spectrum class A beta-lactamase CfxA3	PARTIALX	100.00	83.18	WP_004348227.1
NAFPDFEE_1	3227093	3227824	erm(G)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G)	BLASTX	99.59	100.00	WP_063844791.1
NAFPDFEE_1	2793638	2794435	cfxA3	extended-spectrum class A beta-lactamase CfxA3	PARTIALX	98.87	82.87	WP_004348227.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
NAFPDFEE_1	0.0	1.0	0.0
NAFPDFEE_2	1.0	0.0	1.0
NAFPDFEE_3	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found

Phigaro v2.4.0 (Database: Jan. 2024 release)