Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 3 | 15.91 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JGKKBJEC_1 | 2527650 | 2528504 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| JGKKBJEC_1 | 2509180 | 2510382 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| JGKKBJEC_1 | 1404873 | 1405190 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| JGKKBJEC_1 | 1404535 | 1404873 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| JGKKBJEC_1 | 910472 | 913042 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| JGKKBJEC_1 | 355532 | 356125 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| JGKKBJEC_1 | 306456 | 307889 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JGKKBJEC_1 | 4072920 | 4074296 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.06 | 100.00 | D12567 |
| JGKKBJEC_1 | 2527650 | 2528504 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| JGKKBJEC_1 | 293642 | 294561 | mph(K) | Spiramycin, Telithromycin | 98.05 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JGKKBJEC_1 | 2527653 | 2528504 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| JGKKBJEC_1 | 4072923 | 4074296 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| JGKKBJEC_1 | 603082 | 604689 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 95.61 | 100.00 | WP_003234144.1 |
| JGKKBJEC_1 | 4070376 | 4070894 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| JGKKBJEC_1 | 1982940 | 1985537 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.48 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JGKKBJEC_1 | 3576792 | 3577241 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| JGKKBJEC_1 | 3577256 | 3578437 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| JGKKBJEC_1 | 3575631 | 3576773 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| JGKKBJEC_1 | 3125556 | 3126341 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.11 | 100.00 | NP_391080 |
| JGKKBJEC_1 | 1929554 | 1931317 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| JGKKBJEC_1 | 2111727 | 2112368 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| JGKKBJEC_1 | 3121731 | 3122669 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391077 |
| JGKKBJEC_1 | 3114575 | 3121711 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391076 |
| JGKKBJEC_1 | 3124334 | 3125530 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| JGKKBJEC_1 | 3122697 | 3124316 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JGKKBJEC_1 | 134988 | 136124 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| JGKKBJEC_1 | 1714076 | 1714852 | CodY | 3.35e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JGKKBJEC_1 | 3424436 | 3425005 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JGKKBJEC_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JGKKBJEC_1 | 1346069 | 1375071 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| JGKKBJEC_1 | 2697939 | 2724472 | False | Myoviridae | VOG0221, VOG6116, VOG1301, VOG10547, VOG4574, VOG0136, VOG0753, VOG5008, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720, VOG0796 |
| JGKKBJEC_1 | 3300383 | 3327896 | False | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245 |
| JGKKBJEC_1 | 3328383 | 3338913 | True | Siphoviridae | VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)