safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
IEHJDADO_1	2735032	2735886	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
IEHJDADO_1	2716323	2717525	blt	ARO:3003006	blt	100.00	100.00	L32599.1
IEHJDADO_1	2494005	2495174	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
IEHJDADO_1	1376434	1376751	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
IEHJDADO_1	1376096	1376434	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
IEHJDADO_1	916258	918828	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
IEHJDADO_1	604215	605858	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
IEHJDADO_1	338636	339229	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
IEHJDADO_1	288133	289566	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
IEHJDADO_1	275318	276238	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
IEHJDADO_1	2735032	2735886	aadK	Streptomycin	100.00	100.00	M26879
IEHJDADO_1	275318	276238	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
IEHJDADO_1	4186833	4188209	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
IEHJDADO_1	2735035	2735886	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
IEHJDADO_1	275318	276235	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
IEHJDADO_1	4184315	4184833	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
IEHJDADO_1	4186836	4188209	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
IEHJDADO_1	604218	605858	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
IEHJDADO_1	2050793	2053387	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IEHJDADO_1	3696972	3698114	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
IEHJDADO_1	3698597	3699778	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
IEHJDADO_1	3698133	3698582	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
IEHJDADO_1	2004135	2005898	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
IEHJDADO_1	3290765	3291550	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
IEHJDADO_1	3286929	3287867	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
IEHJDADO_1	3289543	3290739	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
IEHJDADO_1	3287895	3289514	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
IEHJDADO_1	3279773	3286909	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
IEHJDADO_1	2294761	2295402	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IEHJDADO_1	132355	133491	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
IEHJDADO_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
IEHJDADO_1	1689578	1690354	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
IEHJDADO_1	3545488	3546057	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IEHJDADO_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
IEHJDADO_1	1320149	1346257	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
IEHJDADO_1	2666395	2696639	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)