Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOFJFKLM_1 | 2533465 | 2534319 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| DOFJFKLM_1 | 2523261 | 2524463 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| DOFJFKLM_1 | 2342954 | 2344123 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| DOFJFKLM_1 | 1353342 | 1353659 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DOFJFKLM_1 | 1353004 | 1353342 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DOFJFKLM_1 | 895616 | 898186 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| DOFJFKLM_1 | 593298 | 594923 | vmlR | ARO:3004476 | vmlR | 98.15 | 98.90 | NC_000964.3 |
| DOFJFKLM_1 | 328714 | 329307 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DOFJFKLM_1 | 274727 | 276160 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| DOFJFKLM_1 | 264727 | 265647 | mphK | ARO:3004541 | mphK | 91.09 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DOFJFKLM_1 | 2533465 | 2534319 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| DOFJFKLM_1 | 264727 | 265647 | mph(K) | Spiramycin, Telithromycin | 92.85 | 99.78 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DOFJFKLM_1 | 2533468 | 2534319 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| DOFJFKLM_1 | 593301 | 594923 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.15 | 98.90 | WP_003234144.1 |
| DOFJFKLM_1 | 264727 | 265635 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 91.09 | 99.02 | WP_003246254.1 |
| DOFJFKLM_1 | 2036164 | 2038761 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DOFJFKLM_1 | 2140724 | 2141365 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| DOFJFKLM_1 | 3490976 | 3492157 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| DOFJFKLM_1 | 3081913 | 3082698 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| DOFJFKLM_1 | 3490512 | 3490961 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DOFJFKLM_1 | 1987546 | 1989309 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| DOFJFKLM_1 | 3489351 | 3490493 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| DOFJFKLM_1 | 3078076 | 3079014 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.51 | 100.00 | NP_391077 |
| DOFJFKLM_1 | 3070920 | 3078056 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.01 | 100.00 | NP_391076 |
| DOFJFKLM_1 | 3079042 | 3080661 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
| DOFJFKLM_1 | 3080690 | 3081886 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.07 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DOFJFKLM_1 | 132329 | 133465 | tufA | 0.0 | elongation factor Tu | 85.22 | 95.69 | AHM69299 |
| DOFJFKLM_1 | 18597 | 19466 | pdxS | 2.98e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DOFJFKLM_1 | 1664196 | 1664972 | CodY | 3.25e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DOFJFKLM_1 | 3340130 | 3340699 | ClpP | 2.41e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DOFJFKLM_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DOFJFKLM_1 | 1297911 | 1323169 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)