Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 33 | 40.80 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BGDFKAMC_68 | 61311 | 61904 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| BGDFKAMC_3 | 10472 | 11281 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| BGDFKAMC_68 | 61311 | 61904 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| BGDFKAMC_68 | 61311 | 61904 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| BGDFKAMC_3 | 9532 | 10488 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| BGDFKAMC_3 | 13558 | 15342 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.78 | 100.00 | WP_011226325 |
| BGDFKAMC_3 | 8387 | 9535 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| BGDFKAMC_3 | 4040 | 4891 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| BGDFKAMC_3 | 12506 | 13561 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_002948022 |
| BGDFKAMC_3 | 11281 | 12486 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226326 |
| BGDFKAMC_17 | 47515 | 49173 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| BGDFKAMC_68 | 60442 | 61311 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| BGDFKAMC_68 | 105938 | 106727 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 99.87 | - |
| BGDFKAMC_24 | 50782 | 51750 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.97 | 100.00 | WP_082302295 |
| BGDFKAMC_16 | 1586 | 2317 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| BGDFKAMC_3 | 7268 | 8278 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011226330 |
| BGDFKAMC_68 | 25784 | 26545 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| BGDFKAMC_16 | 3028 | 3768 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| BGDFKAMC_16 | 1586 | 2317 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| BGDFKAMC_16 | 1586 | 2317 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| BGDFKAMC_3 | 5994 | 7268 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.20 | 100.00 | WP_011226331 |
| BGDFKAMC_16 | 3028 | 3768 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| BGDFKAMC_16 | 3028 | 3763 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.55 | 98.13 | WP_011227246 |
| BGDFKAMC_16 | 125 | 1585 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.47 | 100.00 | WP_011681177 |
| BGDFKAMC_16 | 125 | 1585 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.58 | 100.21 | WP_011226049 |
| BGDFKAMC_16 | 125 | 1585 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.44 | 100.21 | WP_041827043 |
| BGDFKAMC_16 | 3825 | 5190 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.02 | 99.85 | WP_011681173 |
| BGDFKAMC_16 | 3825 | 5192 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| BGDFKAMC_16 | 2326 | 3018 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.21 | 100.00 | WP_011681175 |
| BGDFKAMC_50 | 97736 | 99039 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.18 | 99.46 | WP_000022832 |
| BGDFKAMC_16 | 2326 | 3018 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 87.59 | 100.00 | WP_011226047 |
| BGDFKAMC_16 | 2326 | 3018 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 87.30 | 100.00 | WP_011227247 |
| BGDFKAMC_63 | 374 | 1384 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.73 | 99.51 | WP_002986042 |
| BGDFKAMC_68 | 62333 | 63379 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| BGDFKAMC_53 | 2345 | 3628 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.50 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BGDFKAMC_68 | 23097 | 22114 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| BGDFKAMC_12 | 6711 | 7784 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| BGDFKAMC_50 | 12234 | 13202 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| BGDFKAMC_9 | 83122 | 85425 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| BGDFKAMC_19 | 5742 | 7988 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| BGDFKAMC_90 | 68119 | 69600 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| BGDFKAMC_66 | 1898 | 900 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| BGDFKAMC_25 | 15300 | 14329 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| BGDFKAMC_10 | 39190 | 38039 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| BGDFKAMC_10 | 11128 | 9140 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| BGDFKAMC_68 | 39291 | 41021 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| BGDFKAMC_48 | 22267 | 20789 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| BGDFKAMC_50 | 97736 | 99037 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| BGDFKAMC_48 | 7072 | 5180 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| BGDFKAMC_19 | 10911 | 9202 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| BGDFKAMC_15 | 2072 | 1062 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| BGDFKAMC_17 | 32659 | 34020 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| BGDFKAMC_57 | 48392 | 46500 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| BGDFKAMC_50 | 83298 | 84188 | bglB | 9.43e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| BGDFKAMC_79 | 1242 | 304 | SP_0676 | 1.85e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| BGDFKAMC_19 | 8180 | 9037 | metF | 1.66e-167 | methionine biosynthesis enzymes | 86.71 | 99.31 | AAK74739 |
| BGDFKAMC_68 | 134426 | 135130 | covR | 3.00e-131 | responder protein | 84.68 | 100.00 | AAK71319 |
| BGDFKAMC_90 | 34409 | 33738 | rr01 | 4.33e-124 | response regulator | 83.04 | 99.56 | CAB54566 |
| BGDFKAMC_19 | 14864 | 15532 | CiaR | 7.54e-123 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| BGDFKAMC_68 | 134426 | 135130 | walR | 2.15e-121 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| BGDFKAMC_19 | 14864 | 15535 | ciaR | 8.67e-121 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| BGDFKAMC_6 | 16143 | 16829 | CovR | 1.28e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| BGDFKAMC_66 | 624 | 229 | SpxA2 | 1.61e-75 | transcriptional regulator | 90.15 | 100.00 | ERL21461 |
| BGDFKAMC_17 | 21296 | 20895 | SpxA1 | 1.06e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| BGDFKAMC_61 | 25421 | 25134 | spr0479 | 2.99e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| BGDFKAMC_24 | 9894 | 9640 | spr1327 | 1.44e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| BGDFKAMC_52 | 26753 | 26490 | spr0175 | 1.97e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| BGDFKAMC_52 | 25950 | 25660 | spr0177 | 1.16e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| BGDFKAMC_53 | 3786 | 4286 | RopE (M5005_Spy_1611) | 1.76e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| BGDFKAMC_9 | 83122 | 85419 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| BGDFKAMC_57 | 33990 | 32971 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BGDFKAMC_3 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_5 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_6 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_7 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_8 | 1.0 | 0.0 | 1.0 |
| BGDFKAMC_9 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_10 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_11 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_12 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_13 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_14 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_15 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_16 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_17 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_18 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_19 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_20 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_21 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_22 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_23 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_24 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_25 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_27 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_29 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_30 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_33 | 1.0 | 0.0 | 1.0 |
| BGDFKAMC_34 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_35 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_36 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_39 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_40 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_48 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_49 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_50 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_51 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_52 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_53 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_54 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_55 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_56 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_57 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_58 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_59 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_60 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_61 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_62 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_63 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_64 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_65 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_66 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_67 | 1.0 | 0.0 | 1.0 |
| BGDFKAMC_68 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_69 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_70 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_73 | 1.0 | 0.0 | 1.0 |
| BGDFKAMC_75 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_77 | 1.0 | 0.0 | 1.0 |
| BGDFKAMC_79 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_80 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_90 | 0.0 | 1.0 | 0.0 |
| BGDFKAMC_101 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BGDFKAMC_35 | 96109 | 98637 | False | Siphoviridae | VOG3436, VOG4649, VOG0054 |
| BGDFKAMC_50 | 100418 | 128060 | False | Siphoviridae | VOG8491, VOG4602, VOG6127, VOG3622, VOG1564, VOG9469, VOG3478, VOG0651, VOG0321, VOG6420, VOG6419, VOG0322, VOG6545, VOG4632, VOG4609, VOG0655, VOG3468, VOG0656, VOG0152, VOG9667, VOG0657, VOG0658, VOG0198, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209 |
Phigaro v2.4.0 (Database: Jan. 2024 release)