Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 27 | 32 | 41.87 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFKAFPOO_16 | 480 | 1319 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| GFKAFPOO_10 | 19731 | 20582 | rfbD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_014621820 |
| GFKAFPOO_10 | 13341 | 14150 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948020 |
| GFKAFPOO_10 | 16344 | 17354 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.90 | 100.00 | WP_011226330 |
| GFKAFPOO_88 | 100921 | 101514 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GFKAFPOO_10 | 14134 | 15090 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| GFKAFPOO_10 | 9280 | 11064 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.72 | 100.00 | WP_011226325 |
| GFKAFPOO_88 | 100921 | 101514 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| GFKAFPOO_88 | 100921 | 101514 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| GFKAFPOO_26 | 13138 | 14796 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| GFKAFPOO_10 | 12136 | 13341 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| GFKAFPOO_10 | 15087 | 16235 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | WP_041828290 |
| GFKAFPOO_10 | 11061 | 12116 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_002948022 |
| GFKAFPOO_88 | 56090 | 56880 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.49 | 100.00 | - |
| GFKAFPOO_88 | 101514 | 102383 | rfbA | 0.0 | Capsule | Immune modulation | 99.31 | 100.00 | WP_011681286 |
| GFKAFPOO_6 | 13826 | 14557 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011227248 |
| GFKAFPOO_88 | 136280 | 137041 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| GFKAFPOO_6 | 18960 | 20375 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681165 |
| GFKAFPOO_6 | 13826 | 14557 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| GFKAFPOO_6 | 15268 | 16008 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011681174 |
| GFKAFPOO_6 | 15268 | 16008 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.98 | 100.00 | WP_011226046 |
| GFKAFPOO_6 | 13826 | 14557 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011681176 |
| GFKAFPOO_6 | 12365 | 13825 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.74 | 100.00 | WP_011681177 |
| GFKAFPOO_10 | 17357 | 18628 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.56 | 99.76 | WP_011226331 |
| GFKAFPOO_6 | 15268 | 16003 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.55 | 98.13 | WP_011227246 |
| GFKAFPOO_6 | 12365 | 13825 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_041827043 |
| GFKAFPOO_6 | 12365 | 13825 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_011226049 |
| GFKAFPOO_6 | 16066 | 17433 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011226045 |
| GFKAFPOO_6 | 16066 | 17431 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.36 | 99.85 | WP_011681173 |
| GFKAFPOO_6 | 30262 | 31253 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 91.39 | 99.70 | - |
| GFKAFPOO_6 | 14566 | 15258 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011681175 |
| GFKAFPOO_6 | 14566 | 15258 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.46 | 100.00 | WP_011226047 |
| GFKAFPOO_11 | 106801 | 108104 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| GFKAFPOO_6 | 14566 | 15258 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 87.88 | 100.00 | WP_011227247 |
| GFKAFPOO_55 | 184 | 1194 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| GFKAFPOO_88 | 99446 | 100492 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| GFKAFPOO_15 | 62966 | 64249 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.42 | 99.77 | WP_000107753 |
| GFKAFPOO_6 | 31262 | 31704 | STU_RS14620 | 2.23e-123 | Capsule | Immune modulation | 84.88 | 98.44 | WP_011226042 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFKAFPOO_88 | 139728 | 140711 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GFKAFPOO_8 | 6844 | 7917 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GFKAFPOO_11 | 24559 | 25527 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GFKAFPOO_10 | 177479 | 179782 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| GFKAFPOO_28 | 6229 | 8475 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| GFKAFPOO_44 | 40262 | 41743 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| GFKAFPOO_16 | 20111 | 21103 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| GFKAFPOO_33 | 15302 | 14331 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| GFKAFPOO_10 | 63592 | 62441 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| GFKAFPOO_10 | 35580 | 33592 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| GFKAFPOO_88 | 123532 | 121802 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| GFKAFPOO_9 | 26998 | 28476 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GFKAFPOO_11 | 106801 | 108102 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GFKAFPOO_9 | 41733 | 43625 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| GFKAFPOO_28 | 11400 | 9691 | PGM | 0.0 | phosphoglucomutase | 83.54 | 99.65 | ACH87041 |
| GFKAFPOO_20 | 18846 | 19856 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GFKAFPOO_34 | 19036 | 17675 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GFKAFPOO_20 | 69166 | 67274 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| GFKAFPOO_11 | 92371 | 93261 | bglB | 5.95e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| GFKAFPOO_35 | 1260 | 325 | SP_0676 | 1.70e-171 | transcription facot | 81.73 | 96.89 | AAK74821 |
| GFKAFPOO_28 | 8667 | 9524 | metF | 1.57e-165 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| GFKAFPOO_88 | 24439 | 23735 | covR | 8.08e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| GFKAFPOO_44 | 21294 | 20623 | rr01 | 1.64e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| GFKAFPOO_88 | 24439 | 23735 | walR | 4.05e-122 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| GFKAFPOO_28 | 15353 | 16009 | CiaR | 1.56e-121 | two-component response regulator | 86.76 | 97.77 | AAK74935 |
| GFKAFPOO_1 | 49048 | 49734 | CovR | 3.06e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GFKAFPOO_28 | 15353 | 16009 | ciaR | 3.11e-119 | two component system response regulator | 84.93 | 96.90 | AIK75708 |
| GFKAFPOO_16 | 21400 | 21795 | SpxA2 | 4.38e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| GFKAFPOO_17 | 25423 | 25136 | spr0479 | 2.15e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| GFKAFPOO_12 | 9898 | 9644 | spr1327 | 4.24e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| GFKAFPOO_9 | 75355 | 75092 | spr0175 | 3.95e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| GFKAFPOO_9 | 74528 | 74262 | spr0177 | 6.97e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| GFKAFPOO_15 | 62808 | 62308 | RopE (M5005_Spy_1611) | 1.76e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| GFKAFPOO_10 | 177479 | 179776 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| GFKAFPOO_20 | 54708 | 53689 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GFKAFPOO_22 | 1201 | 2149 | rep37 | 949 / 948 | 99.47 | AY250830 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFKAFPOO_1 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_2 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_3 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_4 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_5 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_6 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_7 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_8 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_9 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_10 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_11 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_12 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_13 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_14 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_15 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_16 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_17 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_18 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_20 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_21 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_22 | 1.0 | 0.0 | 1.0 |
| GFKAFPOO_23 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_25 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_26 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_27 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_28 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_29 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_30 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_31 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_32 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_33 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_34 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_35 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_37 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_38 | 1.0 | 0.0 | 1.0 |
| GFKAFPOO_39 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_40 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_41 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_42 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_44 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_45 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_46 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_47 | 1.0 | 0.0 | 1.0 |
| GFKAFPOO_48 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_55 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_56 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_57 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_61 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_62 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_63 | 1.0 | 0.0 | 1.0 |
| GFKAFPOO_65 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_66 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_77 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_88 | 0.0 | 1.0 | 0.0 |
| GFKAFPOO_90 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)