Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 34 | 40.50 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AKDCOAKN_33 | 25959 | 27218 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| AKDCOAKN_55 | 96886 | 97479 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| AKDCOAKN_65 | 20684 | 21523 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.88 | 100.00 | WP_011226733 |
| AKDCOAKN_55 | 96886 | 97479 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| AKDCOAKN_55 | 96886 | 97479 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| AKDCOAKN_33 | 27215 | 28191 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| AKDCOAKN_84 | 12114 | 13772 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| AKDCOAKN_33 | 28209 | 29177 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| AKDCOAKN_55 | 132246 | 133007 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| AKDCOAKN_55 | 97479 | 98348 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| AKDCOAKN_55 | 52065 | 52855 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| AKDCOAKN_8 | 16580 | 17311 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| AKDCOAKN_8 | 17312 | 18772 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.49 | 100.00 | WP_011681177 |
| AKDCOAKN_33 | 29179 | 29894 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| AKDCOAKN_33 | 30240 | 31010 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 98.18 | 99.23 | WP_011681440 |
| AKDCOAKN_8 | 16580 | 17311 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011226048 |
| AKDCOAKN_8 | 16580 | 17311 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681176 |
| AKDCOAKN_8 | 17312 | 18772 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.47 | 100.21 | WP_011226049 |
| AKDCOAKN_33 | 24989 | 25840 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| AKDCOAKN_7 | 9476 | 14371 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.88 | 100.00 | WP_000167699 |
| AKDCOAKN_8 | 15129 | 15869 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| AKDCOAKN_8 | 17312 | 18772 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_041827043 |
| AKDCOAKN_8 | 15129 | 15869 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.49 | 100.00 | WP_011226046 |
| AKDCOAKN_8 | 15134 | 15869 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| AKDCOAKN_8 | 15879 | 16571 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011227247 |
| AKDCOAKN_8 | 15879 | 16571 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011226047 |
| AKDCOAKN_7 | 9476 | 14419 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.79 | 99.82 | YP_001200536 |
| AKDCOAKN_7 | 9476 | 14419 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.77 | 99.82 | YP_001198331 |
| AKDCOAKN_8 | 13705 | 15072 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.61 | 100.00 | WP_011226045 |
| AKDCOAKN_8 | 13707 | 15072 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.29 | 99.85 | WP_011681173 |
| AKDCOAKN_8 | 15879 | 16571 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| AKDCOAKN_7 | 47810 | 49113 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| AKDCOAKN_63 | 226 | 1236 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| AKDCOAKN_9 | 62939 | 64222 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| AKDCOAKN_55 | 95411 | 96457 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AKDCOAKN_7 | 14392 | 9479 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.34 | 99.70 | AUS90958 |
| AKDCOAKN_55 | 135694 | 136677 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| AKDCOAKN_77 | 88440 | 89513 | MntE | 0.0 | manganese homeostasis | 94.13 | 99.72 | ABF32167 |
| AKDCOAKN_7 | 132669 | 131701 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| AKDCOAKN_3 | 17045 | 14742 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| AKDCOAKN_15 | 9426 | 7945 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| AKDCOAKN_18 | 6037 | 8283 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| AKDCOAKN_65 | 1893 | 895 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| AKDCOAKN_56 | 136 | 1107 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| AKDCOAKN_4 | 66801 | 67952 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| AKDCOAKN_33 | 11950 | 13938 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| AKDCOAKN_55 | 119498 | 117768 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| AKDCOAKN_22 | 27061 | 28539 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| AKDCOAKN_7 | 49113 | 47812 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| AKDCOAKN_22 | 42284 | 44176 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| AKDCOAKN_18 | 11208 | 9499 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| AKDCOAKN_44 | 55096 | 54086 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| AKDCOAKN_64 | 32204 | 33565 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| AKDCOAKN_44 | 4776 | 6668 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| AKDCOAKN_7 | 63542 | 62652 | bglB | 4.62e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| AKDCOAKN_17 | 42357 | 43295 | SP_0676 | 2.44e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| AKDCOAKN_18 | 8475 | 9332 | metF | 7.85e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| AKDCOAKN_55 | 24424 | 23720 | covR | 5.76e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| AKDCOAKN_15 | 37033 | 37704 | rr01 | 1.79e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| AKDCOAKN_55 | 24424 | 23720 | walR | 2.73e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| AKDCOAKN_2 | 9908 | 9222 | CovR | 3.18e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| AKDCOAKN_18 | 15162 | 15830 | CiaR | 6.50e-119 | two-component response regulator | 83.86 | 99.55 | AAK74935 |
| AKDCOAKN_18 | 15162 | 15833 | ciaR | 6.46e-117 | two component system response regulator | 81.70 | 99.12 | AIK75708 |
| AKDCOAKN_65 | 619 | 224 | SpxA2 | 4.31e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| AKDCOAKN_64 | 21047 | 20646 | SpxA1 | 1.13e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| AKDCOAKN_85 | 25422 | 25135 | spr0479 | 3.33e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| AKDCOAKN_60 | 9899 | 9645 | spr1327 | 2.58e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| AKDCOAKN_22 | 75892 | 75629 | spr0175 | 4.31e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| AKDCOAKN_22 | 75089 | 74799 | spr0177 | 2.53e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| AKDCOAKN_9 | 62781 | 62281 | RopE (M5005_Spy_1611) | 3.67e-76 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| AKDCOAKN_3 | 17045 | 14748 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| AKDCOAKN_44 | 19233 | 20252 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AKDCOAKN_1 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_2 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_3 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_4 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_5 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_6 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_7 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_8 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_9 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_10 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_11 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_13 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_14 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_15 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_16 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_17 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_18 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_19 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_20 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_21 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_22 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_23 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_25 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_26 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_29 | 1.0 | 0.0 | 1.0 |
| AKDCOAKN_30 | 1.0 | 0.0 | 1.0 |
| AKDCOAKN_31 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_33 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_35 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_40 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_41 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_42 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_44 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_46 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_55 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_56 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_59 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_60 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_61 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_62 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_63 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_64 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_65 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_66 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_68 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_70 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_75 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_77 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_84 | 0.0 | 1.0 | 0.0 |
| AKDCOAKN_85 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AKDCOAKN_59 | 17442 | 19680 | False | Unknown | VOG9667, VOG9405, VOG6396 |
Phigaro v2.4.0 (Database: Jan. 2024 release)