Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HJEHFIFL_12 | 25957 | 27216 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| HJEHFIFL_61 | 96884 | 97477 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| HJEHFIFL_27 | 197 | 1036 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.88 | 100.00 | WP_011226733 |
| HJEHFIFL_61 | 96884 | 97477 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HJEHFIFL_61 | 96884 | 97477 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HJEHFIFL_12 | 27213 | 28189 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| HJEHFIFL_56 | 12112 | 13770 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| HJEHFIFL_12 | 28207 | 29175 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| HJEHFIFL_61 | 132244 | 133005 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| HJEHFIFL_61 | 97477 | 98346 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| HJEHFIFL_61 | 52063 | 52853 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| HJEHFIFL_83 | 13142 | 13873 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| HJEHFIFL_83 | 11681 | 13141 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.49 | 100.00 | WP_011681177 |
| HJEHFIFL_12 | 29177 | 29892 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| HJEHFIFL_12 | 30238 | 31008 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 98.18 | 99.23 | WP_011681440 |
| HJEHFIFL_83 | 13142 | 13873 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011226048 |
| HJEHFIFL_83 | 13142 | 13873 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681176 |
| HJEHFIFL_83 | 11681 | 13141 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.47 | 100.21 | WP_011226049 |
| HJEHFIFL_12 | 24987 | 25838 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| HJEHFIFL_11 | 142850 | 147745 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.88 | 100.00 | WP_000167699 |
| HJEHFIFL_83 | 14584 | 15324 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| HJEHFIFL_83 | 11681 | 13141 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_041827043 |
| HJEHFIFL_83 | 14584 | 15324 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.49 | 100.00 | WP_011226046 |
| HJEHFIFL_83 | 14584 | 15319 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| HJEHFIFL_83 | 13882 | 14574 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011227247 |
| HJEHFIFL_83 | 13882 | 14574 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011226047 |
| HJEHFIFL_11 | 142802 | 147745 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.79 | 99.82 | YP_001200536 |
| HJEHFIFL_11 | 142802 | 147745 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.77 | 99.82 | YP_001198331 |
| HJEHFIFL_83 | 15381 | 16748 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.61 | 100.00 | WP_011226045 |
| HJEHFIFL_83 | 15381 | 16746 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.29 | 99.85 | WP_011681173 |
| HJEHFIFL_83 | 13882 | 14574 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| HJEHFIFL_11 | 108108 | 109411 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| HJEHFIFL_34 | 224 | 1234 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| HJEHFIFL_20 | 62937 | 64220 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| HJEHFIFL_61 | 95409 | 96455 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HJEHFIFL_11 | 142829 | 147742 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.34 | 99.70 | AUS90958 |
| HJEHFIFL_61 | 135692 | 136675 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| HJEHFIFL_17 | 88438 | 89511 | MntE | 0.0 | manganese homeostasis | 94.13 | 99.72 | ABF32167 |
| HJEHFIFL_11 | 24552 | 25520 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| HJEHFIFL_19 | 52905 | 55208 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| HJEHFIFL_30 | 70497 | 71978 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| HJEHFIFL_52 | 6622 | 8868 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HJEHFIFL_27 | 19827 | 20825 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| HJEHFIFL_32 | 119 | 1090 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HJEHFIFL_2 | 24286 | 23135 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HJEHFIFL_12 | 11948 | 13936 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| HJEHFIFL_61 | 119496 | 117766 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| HJEHFIFL_3 | 63431 | 61953 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| HJEHFIFL_11 | 108108 | 109409 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HJEHFIFL_3 | 48208 | 46316 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HJEHFIFL_52 | 11793 | 10084 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| HJEHFIFL_7 | 55094 | 54084 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| HJEHFIFL_13 | 32204 | 33565 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| HJEHFIFL_7 | 4774 | 6666 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| HJEHFIFL_11 | 93679 | 94569 | bglB | 4.62e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| HJEHFIFL_23 | 13309 | 12371 | SP_0676 | 2.44e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| HJEHFIFL_52 | 9060 | 9917 | metF | 8.12e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| HJEHFIFL_61 | 24422 | 23718 | covR | 5.76e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| HJEHFIFL_30 | 42890 | 42219 | rr01 | 1.79e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| HJEHFIFL_61 | 24422 | 23718 | walR | 2.73e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| HJEHFIFL_72 | 9906 | 9220 | CovR | 3.18e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HJEHFIFL_52 | 15747 | 16415 | CiaR | 6.73e-119 | two-component response regulator | 83.86 | 99.55 | AAK74935 |
| HJEHFIFL_52 | 15747 | 16418 | ciaR | 6.68e-117 | two component system response regulator | 81.70 | 99.12 | AIK75708 |
| HJEHFIFL_27 | 21101 | 21496 | SpxA2 | 5.35e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| HJEHFIFL_22 | 25420 | 25133 | spr0479 | 3.33e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| HJEHFIFL_1 | 9903 | 9649 | spr1327 | 2.58e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| HJEHFIFL_3 | 14600 | 14863 | spr0175 | 4.31e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| HJEHFIFL_3 | 15403 | 15693 | spr0177 | 2.53e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| HJEHFIFL_20 | 62779 | 62279 | RopE (M5005_Spy_1611) | 3.67e-76 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| HJEHFIFL_19 | 52905 | 55202 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| HJEHFIFL_7 | 19231 | 20250 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HJEHFIFL_1 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_2 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_3 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_5 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_6 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_7 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_9 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_10 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_11 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_12 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_13 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_14 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_15 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_16 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_17 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_18 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_19 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_20 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_21 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_22 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_23 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_25 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_26 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_27 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_28 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_29 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_30 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_31 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_32 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_33 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_34 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_35 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_36 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_37 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_38 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_40 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_41 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_43 | 1.0 | 0.0 | 1.0 |
| HJEHFIFL_44 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_46 | 1.0 | 0.0 | 1.0 |
| HJEHFIFL_48 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_49 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_51 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_52 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_54 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_56 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_57 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_59 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_61 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_63 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_69 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_72 | 0.0 | 1.0 | 0.0 |
| HJEHFIFL_83 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HJEHFIFL_29 | 6979 | 9217 | False | Unknown | VOG6396, VOG6396, VOG9405 |
Phigaro v2.4.0 (Database: Jan. 2024 release)