Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 34 | 41.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HHEELPKI_78 | 96848 | 97441 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| HHEELPKI_78 | 96848 | 97441 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HHEELPKI_78 | 96848 | 97441 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HHEELPKI_21 | 129571 | 130527 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| HHEELPKI_21 | 133597 | 135381 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226325 |
| HHEELPKI_21 | 128426 | 129574 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| HHEELPKI_21 | 130511 | 131320 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.63 | 100.00 | WP_002948020 |
| HHEELPKI_21 | 132545 | 133600 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_002948022 |
| HHEELPKI_21 | 131320 | 132525 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226326 |
| HHEELPKI_3 | 10863 | 12521 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| HHEELPKI_78 | 97441 | 98310 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| HHEELPKI_21 | 124079 | 124930 | rfbD | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | WP_014621820 |
| HHEELPKI_21 | 127307 | 128317 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | WP_011226330 |
| HHEELPKI_42 | 18938 | 19906 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.07 | 100.00 | WP_082302295 |
| HHEELPKI_78 | 132208 | 132969 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| HHEELPKI_78 | 52024 | 52813 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.48 | 99.87 | - |
| HHEELPKI_29 | 1074 | 1814 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011681174 |
| HHEELPKI_21 | 126033 | 127307 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.20 | 100.00 | WP_011226331 |
| HHEELPKI_29 | 1074 | 1814 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011226046 |
| HHEELPKI_29 | 1074 | 1809 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.96 | 98.13 | WP_011227246 |
| HHEELPKI_29 | 372 | 1064 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011227247 |
| HHEELPKI_29 | 372 | 1064 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011226047 |
| HHEELPKI_31 | 9474 | 14369 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.94 | 100.00 | WP_000167699 |
| HHEELPKI_31 | 9474 | 14417 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.77 | 99.82 | YP_001200536 |
| HHEELPKI_31 | 9474 | 14417 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.75 | 99.82 | YP_001198331 |
| HHEELPKI_29 | 372 | 1064 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.63 | 100.00 | WP_011681175 |
| HHEELPKI_31 | 47823 | 49126 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| HHEELPKI_73 | 224 | 1234 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| HHEELPKI_76 | 62901 | 64184 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.50 | 99.77 | WP_000107753 |
| HHEELPKI_78 | 95373 | 96419 | rfbB | 0.0 | Capsule | Immune modulation | 80.42 | 100.00 | WP_009659423 |
| HHEELPKI_38 | 2015 | 2598 | SPD_RS01775 | 3.02e-149 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HHEELPKI_31 | 14390 | 9477 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.34 | 99.70 | AUS90958 |
| HHEELPKI_78 | 135656 | 136639 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| HHEELPKI_24 | 13696 | 12623 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HHEELPKI_8 | 17426 | 19729 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HHEELPKI_31 | 132763 | 131795 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| HHEELPKI_33 | 5739 | 7985 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HHEELPKI_89 | 49167 | 47686 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| HHEELPKI_82 | 19807 | 20805 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| HHEELPKI_6 | 15296 | 14325 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HHEELPKI_21 | 73342 | 74493 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HHEELPKI_21 | 109109 | 111097 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| HHEELPKI_78 | 119460 | 117730 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| HHEELPKI_51 | 72373 | 70895 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| HHEELPKI_31 | 49126 | 47825 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HHEELPKI_51 | 57177 | 55285 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HHEELPKI_33 | 10911 | 9202 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| HHEELPKI_7 | 55051 | 54041 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| HHEELPKI_3 | 36523 | 35171 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.12 | 98.91 | ABP90420 |
| HHEELPKI_7 | 4788 | 6680 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| HHEELPKI_31 | 63559 | 62669 | bglB | 1.13e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| HHEELPKI_52 | 11753 | 10818 | SP_0676 | 2.10e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| HHEELPKI_33 | 8177 | 9034 | metF | 1.34e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| HHEELPKI_78 | 24330 | 23626 | covR | 9.06e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| HHEELPKI_89 | 77220 | 77891 | rr01 | 2.70e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| HHEELPKI_78 | 24330 | 23626 | walR | 4.54e-122 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| HHEELPKI_33 | 14865 | 15533 | CiaR | 5.75e-122 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| HHEELPKI_33 | 14865 | 15536 | ciaR | 5.60e-120 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| HHEELPKI_15 | 7791 | 7105 | CovR | 3.06e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HHEELPKI_82 | 21081 | 21476 | SpxA2 | 4.32e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| HHEELPKI_3 | 47688 | 48089 | SpxA1 | 1.62e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HHEELPKI_13 | 25423 | 25136 | spr0479 | 3.35e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| HHEELPKI_42 | 60793 | 61047 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| HHEELPKI_51 | 14605 | 14868 | spr0175 | 4.77e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| HHEELPKI_51 | 15408 | 15698 | spr0177 | 2.80e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| HHEELPKI_76 | 62743 | 62243 | RopE (M5005_Spy_1611) | 1.76e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HHEELPKI_8 | 17426 | 19723 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| HHEELPKI_7 | 19189 | 20208 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HHEELPKI_1 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_2 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_3 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_5 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_6 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_7 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_8 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_9 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_11 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_12 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_13 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_14 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_15 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_16 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_19 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_20 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_21 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_22 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_23 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_24 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_25 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_26 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_27 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_29 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_31 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_33 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_34 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_35 | 1.0 | 0.0 | 1.0 |
| HHEELPKI_36 | 1.0 | 0.0 | 1.0 |
| HHEELPKI_38 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_39 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_41 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_42 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_44 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_45 | 1.0 | 0.0 | 1.0 |
| HHEELPKI_49 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_51 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_52 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_57 | 1.0 | 0.0 | 1.0 |
| HHEELPKI_62 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_66 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_68 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_71 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_73 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_75 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_76 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_77 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_78 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_79 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_82 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_84 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_85 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_86 | 0.0 | 1.0 | 0.0 |
| HHEELPKI_89 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HHEELPKI_86 | 6979 | 8522 | False | Unknown | VOG6396, VOG6396 |
Phigaro v2.4.0 (Database: Jan. 2024 release)