Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 32 | 40.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NKIHKOBL_90 | 2187 | 3056 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| NKIHKOBL_90 | 1594 | 2187 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| NKIHKOBL_2 | 186965 | 187774 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| NKIHKOBL_90 | 1594 | 2187 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NKIHKOBL_2 | 186025 | 186981 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| NKIHKOBL_2 | 184880 | 186028 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| NKIHKOBL_90 | 1594 | 2187 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| NKIHKOBL_2 | 180533 | 181384 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| NKIHKOBL_68 | 25187 | 26845 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| NKIHKOBL_2 | 187774 | 188979 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| NKIHKOBL_2 | 190051 | 191835 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226325 |
| NKIHKOBL_2 | 183761 | 184771 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| NKIHKOBL_2 | 188999 | 190054 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.34 | 100.00 | WP_002948022 |
| NKIHKOBL_94 | 322 | 1737 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_011681165 |
| NKIHKOBL_11 | 24869 | 25630 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| NKIHKOBL_14 | 502 | 1341 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.69 | 100.00 | WP_011226733 |
| NKIHKOBL_81 | 818 | 1606 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.48 | 99.75 | - |
| NKIHKOBL_20 | 13683 | 14414 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| NKIHKOBL_20 | 13683 | 14414 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011681176 |
| NKIHKOBL_20 | 13683 | 14414 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011227248 |
| NKIHKOBL_2 | 182487 | 183758 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| NKIHKOBL_20 | 12222 | 13682 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 100.00 | WP_011681177 |
| NKIHKOBL_20 | 15125 | 15865 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011681174 |
| NKIHKOBL_20 | 15125 | 15865 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_011226046 |
| NKIHKOBL_20 | 12222 | 13682 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_011226049 |
| NKIHKOBL_20 | 12222 | 13682 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| NKIHKOBL_20 | 15125 | 15860 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.06 | 98.13 | WP_011227246 |
| NKIHKOBL_20 | 15922 | 17285 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.53 | 99.71 | WP_011226045 |
| NKIHKOBL_20 | 14423 | 15115 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| NKIHKOBL_20 | 14423 | 15115 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| NKIHKOBL_20 | 15922 | 17289 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.52 | 99.93 | WP_011681173 |
| NKIHKOBL_20 | 14423 | 15115 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| NKIHKOBL_33 | 95650 | 96953 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| NKIHKOBL_73 | 3719 | 4729 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| NKIHKOBL_75 | 2565 | 3848 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| NKIHKOBL_90 | 119 | 1165 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NKIHKOBL_11 | 22182 | 21199 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NKIHKOBL_13 | 14016 | 12943 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NKIHKOBL_2 | 15566 | 13263 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| NKIHKOBL_33 | 12015 | 12983 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| NKIHKOBL_6 | 51705 | 53186 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| NKIHKOBL_44 | 14145 | 16391 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| NKIHKOBL_14 | 20132 | 21124 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| NKIHKOBL_82 | 119 | 1090 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| NKIHKOBL_2 | 129259 | 130410 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NKIHKOBL_2 | 165563 | 167551 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| NKIHKOBL_11 | 38377 | 40107 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| NKIHKOBL_97 | 27025 | 28503 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| NKIHKOBL_33 | 95650 | 96951 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NKIHKOBL_1 | 6159 | 7868 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| NKIHKOBL_12 | 55130 | 54120 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NKIHKOBL_23 | 30222 | 31583 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| NKIHKOBL_12 | 4763 | 6655 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| NKIHKOBL_33 | 81214 | 82104 | bglB | 4.93e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| NKIHKOBL_77 | 39590 | 40525 | SP_0676 | 2.02e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| NKIHKOBL_44 | 16583 | 17440 | metF | 4.77e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NKIHKOBL_44 | 33764 | 34468 | covR | 1.37e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| NKIHKOBL_6 | 32730 | 32059 | rr01 | 2.03e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| NKIHKOBL_44 | 33764 | 34468 | walR | 5.77e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| NKIHKOBL_1 | 2205 | 1537 | CiaR | 8.50e-120 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| NKIHKOBL_16 | 7718 | 7032 | CovR | 1.33e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NKIHKOBL_1 | 2205 | 1534 | ciaR | 8.36e-118 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| NKIHKOBL_14 | 21421 | 21816 | SpxA2 | 4.40e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| NKIHKOBL_23 | 18859 | 18458 | SpxA1 | 6.40e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NKIHKOBL_70 | 13686 | 13973 | spr0479 | 3.00e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| NKIHKOBL_65 | 5680 | 5426 | spr1327 | 6.89e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| NKIHKOBL_97 | 75858 | 75595 | spr0175 | 3.46e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| NKIHKOBL_97 | 75031 | 74765 | spr0177 | 7.56e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| NKIHKOBL_75 | 4006 | 4506 | RopE (M5005_Spy_1611) | 1.68e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NKIHKOBL_2 | 15566 | 13269 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| NKIHKOBL_12 | 19219 | 20238 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
| NKIHKOBL_97 | 42899 | 44107 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 84.61 | 63.17 | ABP91129 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NKIHKOBL_1 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_2 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_3 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_4 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_6 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_7 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_8 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_9 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_11 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_12 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_13 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_14 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_16 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_17 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_18 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_19 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_20 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_21 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_22 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_23 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_24 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_25 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_26 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_27 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_31 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_32 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_33 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_34 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_41 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_44 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_45 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_46 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_50 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_55 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_57 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_60 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_65 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_68 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_69 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_70 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_71 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_72 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_73 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_74 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_75 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_76 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_77 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_78 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_79 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_80 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_81 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_82 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_84 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_85 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_86 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_87 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_88 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_89 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_90 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_91 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_92 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_93 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_94 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_95 | 1.0 | 0.0 | 1.0 |
| NKIHKOBL_96 | 0.0 | 1.0 | 0.0 |
| NKIHKOBL_97 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)