Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 32 | 40.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MMGLMOIF_57 | 19473 | 20342 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| MMGLMOIF_57 | 20342 | 20935 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| MMGLMOIF_60 | 16051 | 16857 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_011681437 |
| MMGLMOIF_57 | 20342 | 20935 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| MMGLMOIF_60 | 16857 | 17798 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| MMGLMOIF_57 | 20342 | 20935 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| MMGLMOIF_60 | 11698 | 13443 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | WP_011681434 |
| MMGLMOIF_58 | 56731 | 58389 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| MMGLMOIF_60 | 16857 | 17798 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| MMGLMOIF_60 | 17795 | 18943 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | WP_011227431 |
| MMGLMOIF_60 | 16051 | 16857 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011227429 |
| MMGLMOIF_60 | 17795 | 18943 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | WP_011681439 |
| MMGLMOIF_38 | 25846 | 26607 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| MMGLMOIF_16 | 125398 | 126366 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.76 | 100.00 | WP_082302295 |
| MMGLMOIF_55 | 527 | 1942 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.59 | 100.00 | WP_011681165 |
| MMGLMOIF_26 | 13919 | 14650 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| MMGLMOIF_104 | 679 | 1467 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| MMGLMOIF_26 | 13919 | 14650 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011681176 |
| MMGLMOIF_60 | 11698 | 13443 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011227426 |
| MMGLMOIF_60 | 23861 | 24712 | rfbD | 0.0 | Capsule | Immune modulation | 97.89 | 100.00 | WP_014621820 |
| MMGLMOIF_60 | 22481 | 23740 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 97.78 | 100.00 | WP_011681445 |
| MMGLMOIF_26 | 13919 | 14650 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011227248 |
| MMGLMOIF_26 | 12458 | 13918 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681177 |
| MMGLMOIF_26 | 15361 | 16101 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.17 | 100.00 | WP_011226046 |
| MMGLMOIF_26 | 15361 | 16101 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| MMGLMOIF_26 | 12458 | 13918 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.23 | 100.21 | WP_011226049 |
| MMGLMOIF_26 | 15361 | 16096 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| MMGLMOIF_26 | 16158 | 17525 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.47 | 100.00 | WP_011226045 |
| MMGLMOIF_26 | 12458 | 13918 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.41 | 100.21 | WP_041827043 |
| MMGLMOIF_26 | 14659 | 15351 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| MMGLMOIF_26 | 14659 | 15351 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| MMGLMOIF_26 | 16158 | 17523 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.22 | 99.85 | WP_011681173 |
| MMGLMOIF_26 | 14659 | 15351 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| MMGLMOIF_35 | 39828 | 41131 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| MMGLMOIF_56 | 94 | 1104 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| MMGLMOIF_60 | 14828 | 16051 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.52 | 99.92 | WP_012000441 |
| MMGLMOIF_60 | 16051 | 16857 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.78 | 100.00 | WP_012000440 |
| MMGLMOIF_47 | 2712 | 3995 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| MMGLMOIF_57 | 21364 | 22410 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| MMGLMOIF_60 | 21786 | 22484 | STER_RS07095 | 2.90e-162 | Capsule | Immune modulation | 81.86 | 70.35 | WP_011681444 |
| MMGLMOIF_60 | 15314 | 16051 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 97.97 | 61.35 | WP_011681436 |
| MMGLMOIF_60 | 15314 | 16051 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 97.70 | 61.35 | WP_011227428 |
| MMGLMOIF_60 | 20966 | 21432 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.23 | 65.13 | WP_011681442 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MMGLMOIF_100 | 13748 | 12675 | MntE | 0.0 | manganese homeostasis | 94.97 | 99.72 | ABF32167 |
| MMGLMOIF_38 | 23159 | 22176 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| MMGLMOIF_18 | 131461 | 133764 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| MMGLMOIF_16 | 50103 | 49135 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| MMGLMOIF_1 | 11326 | 9080 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| MMGLMOIF_48 | 41072 | 39591 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.50 | 100.00 | ABJ54480 |
| MMGLMOIF_61 | 2044 | 1052 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| MMGLMOIF_45 | 15471 | 14500 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| MMGLMOIF_18 | 17683 | 16532 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| MMGLMOIF_60 | 37885 | 35897 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| MMGLMOIF_38 | 39356 | 41086 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| MMGLMOIF_76 | 26231 | 24753 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| MMGLMOIF_35 | 41131 | 39830 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| MMGLMOIF_76 | 11042 | 9150 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| MMGLMOIF_1 | 6155 | 7864 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| MMGLMOIF_25 | 2068 | 1058 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| MMGLMOIF_58 | 30243 | 31604 | mnmE | 0.0 | central tRNA-modifying GTPase | 81.80 | 99.56 | ABP90420 |
| MMGLMOIF_22 | 24428 | 22536 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| MMGLMOIF_35 | 55559 | 54669 | bglB | 1.37e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| MMGLMOIF_73 | 41120 | 42058 | SP_0676 | 5.65e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| MMGLMOIF_1 | 8888 | 8031 | metF | 4.27e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| MMGLMOIF_104 | 29152 | 29856 | covR | 1.26e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| MMGLMOIF_48 | 60040 | 60711 | rr01 | 1.42e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| MMGLMOIF_104 | 29152 | 29856 | walR | 5.29e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| MMGLMOIF_21 | 6353 | 7039 | CovR | 7.55e-120 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| MMGLMOIF_1 | 2201 | 1533 | CiaR | 8.71e-120 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| MMGLMOIF_1 | 2201 | 1530 | ciaR | 9.44e-118 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| MMGLMOIF_58 | 18884 | 18483 | SpxA1 | 1.45e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| MMGLMOIF_68 | 13683 | 13970 | spr0479 | 2.26e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| MMGLMOIF_16 | 84513 | 84259 | spr1327 | 1.51e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| MMGLMOIF_44 | 14775 | 14512 | spr0175 | 2.12e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| MMGLMOIF_44 | 13948 | 13682 | spr0177 | 3.74e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| MMGLMOIF_47 | 4153 | 4653 | RopE (M5005_Spy_1611) | 1.71e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| MMGLMOIF_18 | 131461 | 133758 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| MMGLMOIF_22 | 9952 | 8933 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MMGLMOIF_1 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_2 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_7 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_12 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_16 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_17 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_18 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_19 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_20 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_21 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_22 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_23 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_25 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_26 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_27 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_28 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_30 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_31 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_32 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_33 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_34 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_35 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_37 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_38 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_39 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_40 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_42 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_43 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_44 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_45 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_46 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_47 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_48 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_49 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_50 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_51 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_52 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_53 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_54 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_55 | 1.0 | 0.0 | 1.0 |
| MMGLMOIF_56 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_57 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_58 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_59 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_60 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_61 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_62 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_64 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_65 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_67 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_68 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_69 | 1.0 | 0.0 | 1.0 |
| MMGLMOIF_70 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_71 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_73 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_76 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_78 | 1.0 | 0.0 | 1.0 |
| MMGLMOIF_79 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_81 | 1.0 | 0.0 | 1.0 |
| MMGLMOIF_82 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_83 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_84 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_85 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_87 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_90 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_100 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_102 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_104 | 0.0 | 1.0 | 0.0 |
| MMGLMOIF_105 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MMGLMOIF_35 | 1817 | 39767 | False | Siphoviridae | VOG3299, VOG4649, VOG0054, VOG3424, VOG3298, VOG4782, VOG0647, VOG4599, VOG4605, VOG3636, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG3435, VOG1887, VOG3434, VOG4555, VOG4564, VOG0720, VOG4544, VOG5060, VOG4618, VOG0198, VOG0658, VOG0657, VOG4571, VOG0656, VOG4693, VOG0226, VOG0685, VOG0283, VOG3430, VOG0189, VOG4566, VOG0651, VOG3478, VOG9469, VOG1564, VOG5353, VOG0743, VOG4602, VOG7656 |
Phigaro v2.4.0 (Database: Jan. 2024 release)