safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	33	47.38

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
BHPDJJLM_16	26707	27558	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
BHPDJJLM_22	65295	66056	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
BHPDJJLM_32	22254	23204	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
BHPDJJLM_32	21147	22229	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
BHPDJJLM_32	20083	21057	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
BHPDJJLM_32	19598	20077	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
BHPDJJLM_32	15997	16596	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
BHPDJJLM_32	15111	15860	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
BHPDJJLM_32	13669	14400	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
BHPDJJLM_22	29938	30531	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
BHPDJJLM_16	14229	15974	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
BHPDJJLM_16	15976	17676	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
BHPDJJLM_16	18896	19702	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
BHPDJJLM_16	19702	20643	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
BHPDJJLM_16	20640	21788	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
BHPDJJLM_37	16808	18466	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
BHPDJJLM_32	24096	25517	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
BHPDJJLM_32	12214	13668	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
BHPDJJLM_16	17694	18896	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
BHPDJJLM_32	18287	19405	STR_RS05230	0.0	Capsule	Immune modulation	99.91	100.00	WP_148148887
BHPDJJLM_32	27211	28269	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
BHPDJJLM_32	16760	17719	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
BHPDJJLM_32	23164	24088	STR_RS05205	0.0	Capsule	Immune modulation	99.89	100.00	WP_011227237
BHPDJJLM_32	14409	15101	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
BHPDJJLM_22	29938	30531	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
BHPDJJLM_22	29938	30531	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
BHPDJJLM_16	15976	17676	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
BHPDJJLM_32	14409	15101	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
BHPDJJLM_16	17694	18896	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
BHPDJJLM_16	18896	19702	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
BHPDJJLM_16	19702	20643	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
BHPDJJLM_17	50800	51768	cbpD	0.0	Autolysin	Exoenzyme	99.38	100.00	WP_082302295
BHPDJJLM_14	52170	52960	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
BHPDJJLM_16	20640	21788	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
BHPDJJLM_22	30531	31400	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
BHPDJJLM_32	13669	14400	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
BHPDJJLM_32	15111	15846	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
BHPDJJLM_32	13669	14400	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
BHPDJJLM_16	14229	15974	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
BHPDJJLM_32	12214	13668	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
BHPDJJLM_32	15111	15846	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
BHPDJJLM_16	25327	26586	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
BHPDJJLM_32	12214	13668	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
BHPDJJLM_32	14409	15101	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
BHPDJJLM_33	107050	108353	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
BHPDJJLM_48	375	1385	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
BHPDJJLM_16	18896	19702	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
BHPDJJLM_22	28463	29509	rfbB	0.0	Capsule	Immune modulation	80.71	100.00	WP_009659423
BHPDJJLM_8	2349	3632	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
BHPDJJLM_16	24632	25330	STER_RS07095	5.34e-154	Capsule	Immune modulation	81.15	70.35	WP_011681444
BHPDJJLM_32	17775	18011	STR_RS05235*	1.06e-120	Capsule	Immune modulation	100.00	100.00	-
BHPDJJLM_16	23812	24278	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
BHPDJJLM_16	18159	18896	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BHPDJJLM_22	68743	69726	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.51	100.00	AAK75326
BHPDJJLM_11	13900	12827	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
BHPDJJLM_33	24814	25782	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
BHPDJJLM_44	10481	8178	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
BHPDJJLM_29	63540	65021	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
BHPDJJLM_39	11332	9086	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
BHPDJJLM_20	19802	20794	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
BHPDJJLM_13	121	1092	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
BHPDJJLM_44	124312	125463	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
BHPDJJLM_44	158785	160767	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
BHPDJJLM_22	52549	50819	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
BHPDJJLM_25	26999	28477	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
BHPDJJLM_33	107050	108351	Eno	0.0	enolase	86.21	99.77	ABP90468
BHPDJJLM_25	41735	43627	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
BHPDJJLM_39	6161	7870	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
BHPDJJLM_55	55035	54025	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
BHPDJJLM_15	7432	6071	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
BHPDJJLM_55	4705	6597	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
BHPDJJLM_33	92620	93510	bglB	2.49e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
BHPDJJLM_31	47015	47950	SP_0676	1.90e-169	transcription facot	81.73	96.89	AAK74821
BHPDJJLM_39	8894	8037	metF	7.68e-167	methionine biosynthesis enzymes	86.36	99.31	AAK74739
BHPDJJLM_14	24471	23767	covR	1.26e-131	responder protein	84.68	100.00	AAK71319
BHPDJJLM_29	29859	29188	rr01	6.55e-127	response regulator	84.38	99.56	CAB54566
BHPDJJLM_14	24471	23767	walR	9.06e-122	DNA-binding response regulator	80.85	100.00	ANC99504
BHPDJJLM_43	16141	16827	CovR	1.40e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
BHPDJJLM_39	2208	1558	CiaR	1.24e-118	two-component response regulator	86.17	96.88	AAK74935
BHPDJJLM_39	2208	1558	ciaR	6.38e-116	two component system response regulator	83.87	96.02	AIK75708
BHPDJJLM_20	21091	21486	SpxA2	4.30e-76	transcriptional regulator	90.91	100.00	ERL21461
BHPDJJLM_15	18790	19191	SpxA1	1.05e-67	transcriptional regulator	80.60	100.00	ERL19273
BHPDJJLM_34	25398	25111	spr0479	3.36e-49	essential gene	86.46	98.97	AAK99283
BHPDJJLM_17	9913	9659	spr1327	1.05e-45	essential gene	95.29	100.00	AAL00131
BHPDJJLM_25	75379	75116	spr0175	4.30e-42	pathogenitcity related	84.09	100.00	AAK98979
BHPDJJLM_25	74552	74286	spr0177	1.11e-33	essential gene	84.27	88.12	AAK98981
BHPDJJLM_8	3790	4290	RopE (M5005_Spy_1611)	3.53e-76	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
BHPDJJLM_44	10481	8184	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
BHPDJJLM_55	19165	20184	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BHPDJJLM_2	0.0	1.0	0.0
BHPDJJLM_3	0.0	1.0	0.0
BHPDJJLM_5	0.0	1.0	0.0
BHPDJJLM_6	0.0	1.0	0.0
BHPDJJLM_7	0.0	1.0	0.0
BHPDJJLM_8	0.0	1.0	0.0
BHPDJJLM_9	0.0	1.0	0.0
BHPDJJLM_10	0.0	1.0	0.0
BHPDJJLM_11	0.0	1.0	0.0
BHPDJJLM_12	0.0	1.0	0.0
BHPDJJLM_13	0.0	1.0	0.0
BHPDJJLM_14	0.0	1.0	0.0
BHPDJJLM_15	0.0	1.0	0.0
BHPDJJLM_16	0.0	1.0	0.0
BHPDJJLM_17	0.0	1.0	0.0
BHPDJJLM_18	0.0	1.0	0.0
BHPDJJLM_20	0.0	1.0	0.0
BHPDJJLM_21	0.0	1.0	0.0
BHPDJJLM_22	0.0	1.0	0.0
BHPDJJLM_23	0.0	1.0	0.0
BHPDJJLM_24	0.0	1.0	0.0
BHPDJJLM_25	0.0	1.0	0.0
BHPDJJLM_27	0.0	1.0	0.0
BHPDJJLM_28	0.0	1.0	0.0
BHPDJJLM_29	0.0	1.0	0.0
BHPDJJLM_30	0.0	1.0	0.0
BHPDJJLM_31	0.0	1.0	0.0
BHPDJJLM_32	0.0	1.0	0.0
BHPDJJLM_33	0.0	1.0	0.0
BHPDJJLM_34	0.0	1.0	0.0
BHPDJJLM_35	0.0	1.0	0.0
BHPDJJLM_36	0.0	1.0	0.0
BHPDJJLM_37	0.0	1.0	0.0
BHPDJJLM_38	1.0	0.0	1.0
BHPDJJLM_39	0.0	1.0	0.0
BHPDJJLM_40	0.0	1.0	0.0
BHPDJJLM_41	0.0	1.0	0.0
BHPDJJLM_42	1.0	0.0	1.0
BHPDJJLM_43	0.0	1.0	0.0
BHPDJJLM_44	0.0	1.0	0.0
BHPDJJLM_45	0.0	1.0	0.0
BHPDJJLM_47	0.0	1.0	0.0
BHPDJJLM_48	0.0	1.0	0.0
BHPDJJLM_49	0.0	1.0	0.0
BHPDJJLM_50	0.0	1.0	0.0
BHPDJJLM_51	1.0	0.0	1.0
BHPDJJLM_52	0.0	1.0	0.0
BHPDJJLM_54	0.0	1.0	0.0
BHPDJJLM_55	0.0	1.0	0.0
BHPDJJLM_56	0.0	1.0	0.0
BHPDJJLM_59	0.0	1.0	0.0
BHPDJJLM_60	0.0	1.0	0.0
BHPDJJLM_61	0.0	1.0	0.0
BHPDJJLM_83	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found