Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 33 | 42.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NCNHKHDH_4 | 52128 | 52918 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 100.00 | 100.00 | - |
| NCNHKHDH_56 | 19729 | 20580 | rfbD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_014621820 |
| NCNHKHDH_4 | 96947 | 97540 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| NCNHKHDH_56 | 14132 | 15088 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226328 |
| NCNHKHDH_56 | 9278 | 11062 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011226325 |
| NCNHKHDH_56 | 13339 | 14148 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| NCNHKHDH_4 | 132306 | 133067 | srtA | 0.0 | Sortase A | Exoenzyme | 99.87 | 100.00 | - |
| NCNHKHDH_4 | 96947 | 97540 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NCNHKHDH_56 | 16342 | 17352 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.80 | 100.00 | WP_011226330 |
| NCNHKHDH_56 | 15085 | 16233 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| NCNHKHDH_56 | 11059 | 12114 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.72 | 100.00 | WP_002948022 |
| NCNHKHDH_60 | 12997 | 14655 | fbp54 | 0.0 | FBPs | Adherence | 99.70 | 100.00 | WP_011227179 |
| NCNHKHDH_4 | 96947 | 97540 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| NCNHKHDH_4 | 97540 | 98409 | rfbA | 0.0 | Capsule | Immune modulation | 99.54 | 100.00 | WP_011681286 |
| NCNHKHDH_61 | 13469 | 14200 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.45 | 100.00 | WP_011681176 |
| NCNHKHDH_61 | 14209 | 14901 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011226047 |
| NCNHKHDH_56 | 12134 | 13339 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011226326 |
| NCNHKHDH_61 | 14209 | 14901 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 98.85 | 100.00 | WP_011227247 |
| NCNHKHDH_10 | 18950 | 19918 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.76 | 100.00 | WP_082302295 |
| NCNHKHDH_25 | 425 | 1840 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.73 | 100.00 | WP_011681165 |
| NCNHKHDH_61 | 12014 | 13468 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.62 | 100.00 | WP_011226049 |
| NCNHKHDH_61 | 12014 | 13468 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.49 | 100.00 | WP_041827043 |
| NCNHKHDH_61 | 14911 | 15651 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011681174 |
| NCNHKHDH_61 | 13469 | 14200 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| NCNHKHDH_61 | 13469 | 14200 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011226048 |
| NCNHKHDH_61 | 14911 | 15646 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.96 | 98.13 | WP_011227246 |
| NCNHKHDH_61 | 14911 | 15651 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| NCNHKHDH_61 | 12014 | 13468 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.60 | 99.79 | WP_011681177 |
| NCNHKHDH_56 | 17352 | 18626 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.57 | 100.00 | WP_011226331 |
| NCNHKHDH_61 | 15708 | 17075 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.69 | 100.00 | WP_011226045 |
| NCNHKHDH_61 | 15708 | 17071 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.94 | 99.71 | WP_011681173 |
| NCNHKHDH_61 | 17304 | 18295 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 92.69 | 99.60 | - |
| NCNHKHDH_61 | 14209 | 14901 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.32 | 100.00 | WP_011681175 |
| NCNHKHDH_54 | 19517 | 20820 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| NCNHKHDH_38 | 3699 | 4709 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| NCNHKHDH_4 | 95471 | 96517 | rfbB | 0.0 | Capsule | Immune modulation | 80.71 | 100.00 | WP_009659423 |
| NCNHKHDH_3 | 2310 | 3593 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NCNHKHDH_4 | 135754 | 136737 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NCNHKHDH_53 | 14019 | 12946 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NCNHKHDH_67 | 24814 | 25782 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| NCNHKHDH_56 | 183776 | 186079 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| NCNHKHDH_22 | 38080 | 36599 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| NCNHKHDH_65 | 11332 | 9086 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| NCNHKHDH_18 | 1976 | 984 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| NCNHKHDH_11 | 15473 | 14502 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| NCNHKHDH_56 | 70040 | 68889 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| NCNHKHDH_56 | 35562 | 33580 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.96 | 99.70 | AE014259_2 |
| NCNHKHDH_4 | 119560 | 117830 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| NCNHKHDH_62 | 236 | 1714 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| NCNHKHDH_54 | 19517 | 20818 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NCNHKHDH_62 | 14972 | 16864 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| NCNHKHDH_65 | 6161 | 7870 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| NCNHKHDH_14 | 18851 | 19861 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NCNHKHDH_66 | 7435 | 6074 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| NCNHKHDH_5 | 4705 | 6597 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| NCNHKHDH_54 | 5087 | 5977 | bglB | 7.39e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| NCNHKHDH_77 | 45976 | 46914 | SP_0676 | 3.90e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| NCNHKHDH_65 | 8894 | 8037 | metF | 1.17e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NCNHKHDH_4 | 24454 | 23750 | covR | 5.79e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| NCNHKHDH_57 | 5309 | 5980 | rr01 | 2.24e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| NCNHKHDH_4 | 24454 | 23750 | walR | 2.74e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| NCNHKHDH_19 | 16149 | 16835 | CovR | 1.40e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NCNHKHDH_65 | 2207 | 1539 | CiaR | 9.68e-119 | two-component response regulator | 83.86 | 99.55 | AAK74935 |
| NCNHKHDH_65 | 2207 | 1536 | ciaR | 9.62e-117 | two component system response regulator | 81.70 | 99.12 | AIK75708 |
| NCNHKHDH_18 | 687 | 292 | SpxA2 | 2.79e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| NCNHKHDH_66 | 18793 | 19194 | SpxA1 | 8.10e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NCNHKHDH_8 | 13395 | 13682 | spr0479 | 3.36e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| NCNHKHDH_10 | 60883 | 61137 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| NCNHKHDH_63 | 14624 | 14887 | spr0175 | 1.59e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| NCNHKHDH_63 | 15451 | 15717 | spr0177 | 4.10e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| NCNHKHDH_3 | 3751 | 4251 | RopE (M5005_Spy_1611) | 3.54e-76 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| NCNHKHDH_56 | 183776 | 186073 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| NCNHKHDH_5 | 19166 | 20185 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NCNHKHDH_1 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_2 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_3 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_4 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_5 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_6 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_7 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_8 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_9 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_10 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_11 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_12 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_13 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_14 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_15 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_16 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_17 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_18 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_19 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_20 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_21 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_22 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_23 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_25 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_26 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_28 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_30 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_31 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_32 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_33 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_35 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_38 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_44 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_53 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_54 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_55 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_56 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_57 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_58 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_59 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_60 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_61 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_62 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_63 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_64 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_65 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_66 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_67 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_68 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_70 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_71 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_72 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_73 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_74 | 1.0 | 0.0 | 1.0 |
| NCNHKHDH_75 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_76 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_77 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_79 | 0.0 | 1.0 | 0.0 |
| NCNHKHDH_80 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)