Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 28 | 32 | 42.52 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LMNBNNPP_10 | 10543 | 11412 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| LMNBNNPP_10 | 9950 | 10543 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| LMNBNNPP_75 | 117926 | 118735 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| LMNBNNPP_10 | 9950 | 10543 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LMNBNNPP_75 | 116986 | 117942 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| LMNBNNPP_75 | 115841 | 116989 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| LMNBNNPP_75 | 118735 | 119940 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.67 | 100.00 | WP_011226326 |
| LMNBNNPP_10 | 9950 | 10543 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| LMNBNNPP_75 | 111494 | 112345 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| LMNBNNPP_77 | 13061 | 14719 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| LMNBNNPP_75 | 121012 | 122796 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226325 |
| LMNBNNPP_75 | 119960 | 121015 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.43 | 100.00 | WP_002948022 |
| LMNBNNPP_75 | 114722 | 115732 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | WP_011226330 |
| LMNBNNPP_1 | 16434 | 17165 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011681176 |
| LMNBNNPP_1 | 17166 | 18626 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.97 | 100.00 | WP_011681177 |
| LMNBNNPP_72 | 44030 | 44818 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| LMNBNNPP_1 | 16434 | 17165 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011227248 |
| LMNBNNPP_1 | 16434 | 17165 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011226048 |
| LMNBNNPP_87 | 25707 | 26468 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| LMNBNNPP_32 | 18955 | 19914 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| LMNBNNPP_75 | 113448 | 114719 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| LMNBNNPP_1 | 17166 | 18626 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.26 | 100.21 | WP_011226049 |
| LMNBNNPP_1 | 14983 | 15723 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.17 | 100.00 | WP_011226046 |
| LMNBNNPP_1 | 14983 | 15723 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| LMNBNNPP_1 | 17166 | 18626 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.72 | 100.21 | WP_041827043 |
| LMNBNNPP_1 | 12321 | 13317 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 96.69 | 100.00 | - |
| LMNBNNPP_1 | 14988 | 15723 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| LMNBNNPP_1 | 13559 | 14926 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.54 | 100.00 | WP_011226045 |
| LMNBNNPP_1 | 15733 | 16425 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| LMNBNNPP_1 | 15733 | 16425 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| LMNBNNPP_1 | 13561 | 14926 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.88 | 99.85 | WP_011681173 |
| LMNBNNPP_1 | 15733 | 16425 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| LMNBNNPP_32 | 178901 | 180204 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| LMNBNNPP_88 | 94 | 1104 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| LMNBNNPP_80 | 59441 | 60724 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| LMNBNNPP_10 | 8475 | 9521 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| LMNBNNPP_1 | 11863 | 12312 | STU_RS14620 | 2.95e-179 | Capsule | Immune modulation | 92.00 | 100.00 | WP_011226042 |
| LMNBNNPP_1 | 2277 | 2860 | SPD_RS01775 | 2.38e-150 | Capsule | Immune modulation | 83.25 | 96.20 | WP_001819836 |
| LMNBNNPP_1 | 11502 | 11863 | STU_RS14615 | 1.21e-83 | Capsule | Immune modulation | 82.51 | 73.87 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LMNBNNPP_87 | 23020 | 22037 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| LMNBNNPP_3 | 13779 | 12706 | MntE | 0.0 | manganese homeostasis | 94.13 | 99.72 | ABF32167 |
| LMNBNNPP_32 | 95220 | 96188 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| LMNBNNPP_6 | 15568 | 13265 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| LMNBNNPP_90 | 41703 | 40222 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| LMNBNNPP_52 | 29786 | 27540 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| LMNBNNPP_8 | 1905 | 904 | recA | 0.0 | DNA recombination and repair | 91.02 | 86.08 | AAL00560 |
| LMNBNNPP_22 | 119 | 1090 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| LMNBNNPP_75 | 61012 | 62163 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| LMNBNNPP_75 | 96505 | 98493 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| LMNBNNPP_87 | 39216 | 40946 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| LMNBNNPP_47 | 80206 | 78728 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| LMNBNNPP_32 | 178901 | 180202 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| LMNBNNPP_47 | 65017 | 63125 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| LMNBNNPP_52 | 24615 | 26324 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| LMNBNNPP_93 | 19123 | 20133 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| LMNBNNPP_20 | 30226 | 31587 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| LMNBNNPP_93 | 69391 | 67499 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| LMNBNNPP_32 | 164466 | 165356 | bglB | 1.84e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| LMNBNNPP_76 | 11800 | 10865 | SP_0676 | 1.92e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| LMNBNNPP_52 | 27348 | 26491 | metF | 3.52e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| LMNBNNPP_72 | 72500 | 73204 | covR | 2.28e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| LMNBNNPP_90 | 60678 | 61349 | rr01 | 1.42e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| LMNBNNPP_72 | 72500 | 73204 | walR | 9.61e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| LMNBNNPP_83 | 16879 | 17565 | CovR | 4.03e-120 | serves to repress GAS virulence factor-encoding genes | 81.22 | 100.00 | ACE75886 |
| LMNBNNPP_52 | 20661 | 19993 | CiaR | 4.67e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| LMNBNNPP_52 | 20661 | 19990 | ciaR | 4.60e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| LMNBNNPP_8 | 631 | 236 | SpxA2 | 7.67e-76 | transcriptional regulator | 90.15 | 100.00 | ERL21461 |
| LMNBNNPP_62 | 16295 | 16582 | spr0479 | 3.20e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| LMNBNNPP_32 | 60805 | 61059 | spr1327 | 2.67e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| LMNBNNPP_47 | 31393 | 31656 | spr0175 | 4.13e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| LMNBNNPP_47 | 32220 | 32486 | spr0177 | 9.03e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| LMNBNNPP_80 | 59283 | 58783 | RopE (M5005_Spy_1611) | 1.67e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| LMNBNNPP_6 | 15568 | 13271 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| LMNBNNPP_93 | 54984 | 53965 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LMNBNNPP_1 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_2 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_3 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_4 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_5 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_6 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_7 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_8 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_9 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_10 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_11 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_12 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_14 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_15 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_16 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_17 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_18 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_19 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_20 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_21 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_22 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_25 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_26 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_27 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_28 | 1.0 | 0.0 | 1.0 |
| LMNBNNPP_29 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_32 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_33 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_34 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_43 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_47 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_48 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_52 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_62 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_71 | 1.0 | 0.0 | 1.0 |
| LMNBNNPP_72 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_73 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_75 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_76 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_77 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_79 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_80 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_81 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_82 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_83 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_84 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_85 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_86 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_87 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_88 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_90 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_91 | 0.0 | 1.0 | 0.0 |
| LMNBNNPP_93 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)