safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	33	47.38

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
GLGHBANG_24	20692	21531	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
GLGHBANG_8	26708	27559	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
GLGHBANG_3	23227	24150	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
GLGHBANG_3	22317	23267	STR_RS05210	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227238
GLGHBANG_3	21210	22292	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
GLGHBANG_3	20146	21120	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
GLGHBANG_3	19661	20140	STR_RS05225	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827041
GLGHBANG_3	18350	19468	STR_RS05230	0.0	Capsule	Immune modulation	100.00	100.00	WP_148148887
GLGHBANG_3	16060	16659	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
GLGHBANG_3	15174	15923	STR_RS05250	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227246
GLGHBANG_3	13732	14463	STR_RS05260	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227248
GLGHBANG_6	29938	30531	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
GLGHBANG_8	14229	15974	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
GLGHBANG_8	15976	17676	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
GLGHBANG_8	18896	19702	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
GLGHBANG_8	19702	20643	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
GLGHBANG_8	20640	21788	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
GLGHBANG_32	5371	7029	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
GLGHBANG_3	24158	25579	STR_RS05200	0.0	Capsule	Immune modulation	99.93	100.00	WP_011227236
GLGHBANG_3	12277	13731	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
GLGHBANG_8	17694	18896	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
GLGHBANG_3	27273	28331	waaB	0.0	Capsule	Immune modulation	99.91	100.00	WP_157822584
GLGHBANG_3	16823	17782	STR_RS05240	0.0	Capsule	Immune modulation	99.90	100.00	WP_041827042
GLGHBANG_3	14472	15164	STR_RS05255	0.0	Capsule	Immune modulation	99.86	100.00	WP_011227247
GLGHBANG_6	29938	30531	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
GLGHBANG_6	29938	30531	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
GLGHBANG_6	65295	66054	srtA	0.0	Sortase A	Exoenzyme	99.74	99.87	-
GLGHBANG_8	15976	17676	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
GLGHBANG_3	14472	15164	STU_RS14640	0.0	Capsule	Immune modulation	99.57	100.00	WP_011226047
GLGHBANG_8	17694	18896	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
GLGHBANG_8	18896	19702	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
GLGHBANG_8	19702	20643	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
GLGHBANG_20	14291	15081	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
GLGHBANG_8	20640	21788	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
GLGHBANG_6	30531	31400	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
GLGHBANG_3	13732	14463	STU_RS14645	0.0	Capsule	Immune modulation	98.77	100.00	WP_011226048
GLGHBANG_3	15174	15909	STER_RS05305	0.0	Capsule	Immune modulation	98.50	99.33	WP_011681174
GLGHBANG_3	13732	14463	STER_RS05315	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681176
GLGHBANG_8	14229	15974	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
GLGHBANG_3	12277	13731	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
GLGHBANG_3	15174	15909	STU_RS14635	0.0	Capsule	Immune modulation	97.96	99.33	WP_011226046
GLGHBANG_8	25327	26586	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
GLGHBANG_3	12277	13731	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
GLGHBANG_3	14472	15164	STER_RS05310	0.0	Capsule	Immune modulation	89.75	100.00	WP_011681175
GLGHBANG_33	107083	108386	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
GLGHBANG_25	14302	15312	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
GLGHBANG_8	18896	19702	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
GLGHBANG_21	2349	3632	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
GLGHBANG_6	28463	29509	rfbB	0.0	Capsule	Immune modulation	80.52	100.00	WP_009659423
GLGHBANG_8	24632	25330	STER_RS07095	5.34e-154	Capsule	Immune modulation	81.15	70.35	WP_011681444
GLGHBANG_3	17838	18074	STR_RS05235*	1.06e-120	Capsule	Immune modulation	100.00	100.00	-
GLGHBANG_8	23812	24278	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
GLGHBANG_8	18159	18896	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
GLGHBANG_6	68741	69724	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
GLGHBANG_9	59221	60294	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
GLGHBANG_33	24810	25778	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
GLGHBANG_77	10481	8178	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
GLGHBANG_14	13742	15988	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
GLGHBANG_90	38075	36594	SPD_0310	0.0	UPF0371 protein SPD_0310	91.90	100.00	ABJ54480
GLGHBANG_24	1900	908	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
GLGHBANG_57	130	1101	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
GLGHBANG_77	124338	125489	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
GLGHBANG_77	158811	160793	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
GLGHBANG_6	52549	50819	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.69	100.00	AAK34199
GLGHBANG_1	26999	28477	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
GLGHBANG_33	107083	108384	Eno	0.0	enolase	86.21	99.77	ABP90468
GLGHBANG_1	41735	43627	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
GLGHBANG_14	18913	17204	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
GLGHBANG_4	18873	19883	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
GLGHBANG_7	44544	45905	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
GLGHBANG_4	69204	67312	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
GLGHBANG_33	92653	93543	bglB	2.09e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
GLGHBANG_26	1244	309	SP_0676	1.90e-169	transcription facot	81.73	96.89	AAK74821
GLGHBANG_14	16180	17037	metF	1.14e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
GLGHBANG_20	42829	43533	covR	1.26e-131	responder protein	84.68	100.00	AAK71319
GLGHBANG_34	29860	29189	rr01	5.07e-127	response regulator	84.38	99.56	CAB54566
GLGHBANG_20	42829	43533	walR	9.07e-122	DNA-binding response regulator	80.85	100.00	ANC99504
GLGHBANG_5	10111	9425	CovR	1.70e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
GLGHBANG_14	22866	23516	CiaR	1.84e-118	two-component response regulator	86.17	96.88	AAK74935
GLGHBANG_14	22866	23516	ciaR	9.48e-116	two component system response regulator	83.87	96.02	AIK75708
GLGHBANG_24	611	216	SpxA2	4.30e-76	transcriptional regulator	90.91	100.00	ERL21461
GLGHBANG_7	33185	32784	SpxA1	1.05e-67	transcriptional regulator	80.60	100.00	ERL19273
GLGHBANG_80	25398	25111	spr0479	3.35e-49	essential gene	86.46	98.97	AAK99283
GLGHBANG_88	9906	9652	spr1327	1.05e-45	essential gene	95.29	100.00	AAL00131
GLGHBANG_1	75379	75116	spr0175	4.30e-42	pathogenitcity related	84.09	100.00	AAK98979
GLGHBANG_1	74552	74286	spr0177	1.11e-33	essential gene	84.27	88.12	AAK98981
GLGHBANG_21	3790	4290	RopE (M5005_Spy_1611)	3.53e-76	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
GLGHBANG_77	10481	8184	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
GLGHBANG_4	54743	53724	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
GLGHBANG_1	0.0	1.0	0.0
GLGHBANG_2	0.0	1.0	0.0
GLGHBANG_3	0.0	1.0	0.0
GLGHBANG_4	0.0	1.0	0.0
GLGHBANG_5	0.0	1.0	0.0
GLGHBANG_6	0.0	1.0	0.0
GLGHBANG_7	0.0	1.0	0.0
GLGHBANG_8	0.0	1.0	0.0
GLGHBANG_9	0.0	1.0	0.0
GLGHBANG_10	0.0	1.0	0.0
GLGHBANG_11	0.0	1.0	0.0
GLGHBANG_12	0.0	1.0	0.0
GLGHBANG_13	0.0	1.0	0.0
GLGHBANG_14	0.0	1.0	0.0
GLGHBANG_15	0.0	1.0	0.0
GLGHBANG_16	0.0	1.0	0.0
GLGHBANG_17	1.0	0.0	1.0
GLGHBANG_19	0.0	1.0	0.0
GLGHBANG_20	0.0	1.0	0.0
GLGHBANG_21	0.0	1.0	0.0
GLGHBANG_22	0.0	1.0	0.0
GLGHBANG_23	0.0	1.0	0.0
GLGHBANG_24	0.0	1.0	0.0
GLGHBANG_25	0.0	1.0	0.0
GLGHBANG_26	0.0	1.0	0.0
GLGHBANG_27	0.0	1.0	0.0
GLGHBANG_28	0.0	1.0	0.0
GLGHBANG_29	0.0	1.0	0.0
GLGHBANG_30	0.0	1.0	0.0
GLGHBANG_31	0.0	1.0	0.0
GLGHBANG_32	0.0	1.0	0.0
GLGHBANG_33	0.0	1.0	0.0
GLGHBANG_34	0.0	1.0	0.0
GLGHBANG_35	0.0	1.0	0.0
GLGHBANG_36	0.0	1.0	0.0
GLGHBANG_37	0.0	1.0	0.0
GLGHBANG_38	0.0	1.0	0.0
GLGHBANG_39	0.0	1.0	0.0
GLGHBANG_40	0.0	1.0	0.0
GLGHBANG_41	0.0	1.0	0.0
GLGHBANG_42	0.0	1.0	0.0
GLGHBANG_43	0.0	1.0	0.0
GLGHBANG_44	0.0	1.0	0.0
GLGHBANG_45	0.0	1.0	0.0
GLGHBANG_46	0.0	1.0	0.0
GLGHBANG_50	1.0	0.0	1.0
GLGHBANG_52	0.0	1.0	0.0
GLGHBANG_55	0.0	1.0	0.0
GLGHBANG_57	0.0	1.0	0.0
GLGHBANG_60	0.0	1.0	0.0
GLGHBANG_66	0.0	1.0	0.0
GLGHBANG_77	0.0	1.0	0.0
GLGHBANG_80	0.0	1.0	0.0
GLGHBANG_83	1.0	0.0	1.0
GLGHBANG_88	0.0	1.0	0.0
GLGHBANG_89	0.0	1.0	0.0
GLGHBANG_90	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found