Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 21 | 32 | 38.28 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KLMHIIEB_63 | 105790 | 106580 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 100.00 | 100.00 | - |
| KLMHIIEB_22 | 180266 | 181117 | rfbD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_014621820 |
| KLMHIIEB_63 | 61168 | 61761 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| KLMHIIEB_22 | 189784 | 191568 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226325 |
| KLMHIIEB_22 | 187507 | 188712 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226326 |
| KLMHIIEB_22 | 186698 | 187507 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948020 |
| KLMHIIEB_22 | 185758 | 186714 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226328 |
| KLMHIIEB_22 | 184613 | 185761 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_041828290 |
| KLMHIIEB_22 | 183494 | 184504 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226330 |
| KLMHIIEB_22 | 182220 | 183494 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226331 |
| KLMHIIEB_22 | 188732 | 189787 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.91 | 100.00 | WP_002948022 |
| KLMHIIEB_63 | 25641 | 26402 | srtA | 0.0 | Sortase A | Exoenzyme | 99.87 | 100.00 | - |
| KLMHIIEB_7 | 6529 | 7983 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 99.86 | 100.00 | WP_041827043 |
| KLMHIIEB_63 | 61168 | 61761 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| KLMHIIEB_63 | 61168 | 61761 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| KLMHIIEB_14 | 189 | 1028 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.64 | 100.00 | WP_011226733 |
| KLMHIIEB_5 | 13061 | 14719 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| KLMHIIEB_63 | 60299 | 61168 | rfbA | 0.0 | Capsule | Immune modulation | 99.54 | 100.00 | WP_011681286 |
| KLMHIIEB_7 | 6529 | 7983 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011226049 |
| KLMHIIEB_7 | 6529 | 7983 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.92 | 99.79 | WP_011681177 |
| KLMHIIEB_75 | 82215 | 83518 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| KLMHIIEB_9 | 3699 | 4709 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| KLMHIIEB_63 | 62191 | 63237 | rfbB | 0.0 | Capsule | Immune modulation | 80.71 | 100.00 | WP_009659423 |
| KLMHIIEB_24 | 63133 | 64416 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| KLMHIIEB_7 | 2558 | 3141 | SPD_RS01775 | 1.20e-148 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
| KLMHIIEB_7 | 6064 | 6528 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.78 | 63.52 | WP_011227248 |
| KLMHIIEB_7 | 6064 | 6528 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.28 | 63.52 | WP_011226048 |
| KLMHIIEB_7 | 6064 | 6528 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.20 | 63.52 | WP_011681176 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KLMHIIEB_63 | 22954 | 21971 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| KLMHIIEB_28 | 24360 | 25433 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| KLMHIIEB_75 | 165755 | 164787 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| KLMHIIEB_22 | 17048 | 14745 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| KLMHIIEB_29 | 11334 | 9088 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| KLMHIIEB_66 | 19481 | 20962 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| KLMHIIEB_73 | 12168 | 13160 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| KLMHIIEB_86 | 29 | 1000 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| KLMHIIEB_22 | 130788 | 131939 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| KLMHIIEB_22 | 165284 | 167266 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.96 | 99.70 | AE014259_2 |
| KLMHIIEB_63 | 39148 | 40878 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| KLMHIIEB_2 | 236 | 1714 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| KLMHIIEB_75 | 83518 | 82217 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| KLMHIIEB_2 | 14972 | 16864 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| KLMHIIEB_29 | 6163 | 7872 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| KLMHIIEB_12 | 18874 | 19884 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KLMHIIEB_11 | 7435 | 6074 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| KLMHIIEB_1 | 4700 | 6592 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| KLMHIIEB_75 | 97948 | 97058 | bglB | 1.37e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| KLMHIIEB_18 | 47230 | 48165 | SP_0676 | 2.39e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| KLMHIIEB_29 | 8896 | 8039 | metF | 1.17e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| KLMHIIEB_63 | 134259 | 134963 | covR | 2.99e-131 | responder protein | 84.68 | 100.00 | AAK71319 |
| KLMHIIEB_31 | 5309 | 5980 | rr01 | 2.24e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| KLMHIIEB_63 | 134259 | 134963 | walR | 2.15e-121 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| KLMHIIEB_62 | 16112 | 16798 | CovR | 1.40e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| KLMHIIEB_29 | 2209 | 1541 | CiaR | 9.68e-119 | two-component response regulator | 83.86 | 99.55 | AAK74935 |
| KLMHIIEB_29 | 2209 | 1538 | ciaR | 9.62e-117 | two component system response regulator | 81.70 | 99.12 | AIK75708 |
| KLMHIIEB_73 | 13457 | 13852 | SpxA2 | 2.79e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| KLMHIIEB_8 | 25568 | 25281 | spr0479 | 3.37e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| KLMHIIEB_61 | 60661 | 60915 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| KLMHIIEB_43 | 14625 | 14888 | spr0175 | 1.59e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| KLMHIIEB_43 | 15452 | 15718 | spr0177 | 4.10e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| KLMHIIEB_24 | 62975 | 62475 | RopE (M5005_Spy_1611) | 3.54e-76 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| KLMHIIEB_22 | 17048 | 14751 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| KLMHIIEB_1 | 19161 | 20180 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KLMHIIEB_34 | 134 | 1081 | rep37 | 950 / 948 | 94.74 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KLMHIIEB_1 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_2 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_3 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_4 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_5 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_6 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_7 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_8 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_9 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_10 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_11 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_12 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_13 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_14 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_15 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_16 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_17 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_18 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_19 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_21 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_22 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_24 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_26 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_27 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_28 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_29 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_30 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_31 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_32 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_33 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_34 | 1.0 | 0.0 | 1.0 |
| KLMHIIEB_35 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_36 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_37 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_38 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_39 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_41 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_43 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_47 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_57 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_61 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_62 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_63 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_64 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_65 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_66 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_67 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_68 | 1.0 | 0.0 | 1.0 |
| KLMHIIEB_69 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_71 | 1.0 | 0.0 | 1.0 |
| KLMHIIEB_73 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_75 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_76 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_78 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_79 | 1.0 | 0.0 | 1.0 |
| KLMHIIEB_81 | 1.0 | 0.0 | 1.0 |
| KLMHIIEB_86 | 0.0 | 1.0 | 0.0 |
| KLMHIIEB_87 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)