Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 32 | 38.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MNIJHHAM_77 | 31551 | 32810 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| MNIJHHAM_77 | 29591 | 30559 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011681443 |
| MNIJHHAM_1 | 31061 | 31930 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| MNIJHHAM_1 | 31930 | 32523 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226149 |
| MNIJHHAM_77 | 30577 | 31554 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681444 |
| MNIJHHAM_1 | 76554 | 77344 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.24 | 100.00 | - |
| MNIJHHAM_90 | 56878 | 58536 | fbp54 | 0.0 | FBPs | Adherence | 99.22 | 100.00 | WP_011227179 |
| MNIJHHAM_1 | 31930 | 32523 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| MNIJHHAM_1 | 31930 | 32523 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.16 | 100.00 | WP_011226149 |
| MNIJHHAM_17 | 3765 | 4496 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| MNIJHHAM_88 | 2943 | 3704 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| MNIJHHAM_5 | 18975 | 19943 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.76 | 100.00 | WP_082302295 |
| MNIJHHAM_17 | 3765 | 4496 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| MNIJHHAM_17 | 3765 | 4496 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| MNIJHHAM_17 | 4497 | 5957 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011681177 |
| MNIJHHAM_77 | 28874 | 29589 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.18 | 99.86 | WP_011681442 |
| MNIJHHAM_80 | 381 | 1796 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| MNIJHHAM_17 | 3064 | 3756 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011227247 |
| MNIJHHAM_17 | 3064 | 3756 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011226047 |
| MNIJHHAM_77 | 27752 | 28528 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681440 |
| MNIJHHAM_17 | 2316 | 3054 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.56 | 99.73 | WP_011681174 |
| MNIJHHAM_77 | 32929 | 33780 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| MNIJHHAM_17 | 4497 | 5957 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.19 | 100.21 | WP_011226049 |
| MNIJHHAM_17 | 2316 | 3054 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.89 | 99.73 | WP_011226046 |
| MNIJHHAM_17 | 2319 | 3054 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.74 | 98.13 | WP_011227246 |
| MNIJHHAM_17 | 4497 | 5957 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.37 | 100.21 | WP_041827043 |
| MNIJHHAM_17 | 889 | 2256 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.88 | 100.00 | WP_011226045 |
| MNIJHHAM_17 | 891 | 2256 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.29 | 99.85 | WP_011681173 |
| MNIJHHAM_17 | 3064 | 3756 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.04 | 100.00 | WP_011681175 |
| MNIJHHAM_33 | 106777 | 108080 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| MNIJHHAM_15 | 464 | 1474 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| MNIJHHAM_1 | 32953 | 33999 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| MNIJHHAM_6 | 62903 | 64186 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.19 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MNIJHHAM_88 | 6391 | 7374 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| MNIJHHAM_33 | 213357 | 214430 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| MNIJHHAM_33 | 24817 | 25785 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| MNIJHHAM_14 | 15577 | 13274 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| MNIJHHAM_33 | 243809 | 246055 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| MNIJHHAM_9 | 9396 | 7915 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| MNIJHHAM_68 | 1887 | 889 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| MNIJHHAM_61 | 15299 | 14328 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| MNIJHHAM_77 | 75007 | 73856 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| MNIJHHAM_77 | 47029 | 45041 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| MNIJHHAM_1 | 9899 | 11629 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| MNIJHHAM_44 | 27432 | 28910 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| MNIJHHAM_44 | 42166 | 44058 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| MNIJHHAM_33 | 106777 | 108078 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| MNIJHHAM_33 | 248980 | 247271 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| MNIJHHAM_19 | 19104 | 20114 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| MNIJHHAM_90 | 32997 | 34358 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| MNIJHHAM_33 | 92350 | 93240 | bglB | 1.83e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| MNIJHHAM_66 | 39676 | 40611 | SP_0676 | 2.03e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| MNIJHHAM_33 | 246247 | 247104 | metF | 6.39e-165 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| MNIJHHAM_1 | 105031 | 105735 | covR | 3.05e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| MNIJHHAM_9 | 37511 | 38182 | rr01 | 1.51e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| MNIJHHAM_1 | 105031 | 105735 | walR | 1.28e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| MNIJHHAM_7 | 48966 | 49652 | CovR | 3.92e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| MNIJHHAM_33 | 252934 | 253602 | CiaR | 1.29e-118 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| MNIJHHAM_33 | 252934 | 253605 | ciaR | 1.40e-116 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| MNIJHHAM_68 | 613 | 239 | SpxA2 | 7.62e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| MNIJHHAM_90 | 21638 | 21237 | SpxA1 | 1.19e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| MNIJHHAM_18 | 13136 | 13423 | spr0479 | 2.95e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| MNIJHHAM_5 | 60830 | 61084 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| MNIJHHAM_55 | 16887 | 16624 | spr0175 | 2.37e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| MNIJHHAM_55 | 16060 | 15794 | spr0177 | 4.19e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| MNIJHHAM_6 | 62745 | 62245 | RopE (M5005_Spy_1611) | 1.76e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| MNIJHHAM_14 | 15577 | 13280 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| MNIJHHAM_19 | 54966 | 53947 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MNIJHHAM_1 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_2 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_3 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_4 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_5 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_6 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_7 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_8 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_9 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_10 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_11 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_12 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_13 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_14 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_15 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_17 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_18 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_19 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_20 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_21 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_22 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_23 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_24 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_25 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_27 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_28 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_29 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_30 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_33 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_34 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_36 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_41 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_44 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_49 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_54 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_55 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_59 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_60 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_61 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_62 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_63 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_64 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_65 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_66 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_68 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_70 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_72 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_73 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_76 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_77 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_78 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_79 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_80 | 1.0 | 0.0 | 1.0 |
| MNIJHHAM_84 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_88 | 0.0 | 1.0 | 0.0 |
| MNIJHHAM_90 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)