Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFPEAKKP_38 | 9711 | 10580 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| GFPEAKKP_38 | 9118 | 9711 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| GFPEAKKP_5 | 13980 | 14789 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| GFPEAKKP_38 | 9118 | 9711 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GFPEAKKP_38 | 9118 | 9711 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| GFPEAKKP_5 | 14773 | 15729 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226328 |
| GFPEAKKP_55 | 9204 | 10043 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| GFPEAKKP_5 | 9919 | 11703 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| GFPEAKKP_42 | 14025 | 15683 | fbp54 | 0.0 | FBPs | Adherence | 99.34 | 100.00 | WP_011227179 |
| GFPEAKKP_5 | 12775 | 13980 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.25 | 100.00 | WP_011226326 |
| GFPEAKKP_5 | 11700 | 12755 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_002948022 |
| GFPEAKKP_5 | 21909 | 22760 | rfbD | 0.0 | Capsule | Immune modulation | 99.06 | 100.00 | WP_014621820 |
| GFPEAKKP_51 | 7200 | 7931 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011681176 |
| GFPEAKKP_51 | 7200 | 7931 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011226048 |
| GFPEAKKP_5 | 18570 | 19532 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.65 | 95.25 | WP_011226330 |
| GFPEAKKP_51 | 7200 | 7931 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| GFPEAKKP_5 | 15726 | 16874 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.43 | 100.00 | WP_041828290 |
| GFPEAKKP_89 | 44022 | 44810 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| GFPEAKKP_71 | 1208 | 2623 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.74 | 100.00 | WP_011681165 |
| GFPEAKKP_15 | 26257 | 27018 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| GFPEAKKP_5 | 19535 | 20806 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.64 | 99.76 | WP_011226331 |
| GFPEAKKP_51 | 7932 | 9392 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.47 | 100.00 | WP_011681177 |
| GFPEAKKP_51 | 7932 | 9392 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_011226049 |
| GFPEAKKP_51 | 7932 | 9392 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.89 | 100.21 | WP_041827043 |
| GFPEAKKP_51 | 6499 | 7191 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| GFPEAKKP_51 | 6499 | 7191 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| GFPEAKKP_51 | 4309 | 5674 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.17 | 99.85 | WP_011681173 |
| GFPEAKKP_51 | 4307 | 5674 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226045 |
| GFPEAKKP_51 | 5731 | 6489 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.81 | 100.94 | WP_011681174 |
| GFPEAKKP_51 | 5731 | 6489 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.68 | 100.94 | WP_011226046 |
| GFPEAKKP_51 | 5736 | 6489 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.25 | 100.00 | WP_011227246 |
| GFPEAKKP_51 | 6499 | 7191 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| GFPEAKKP_29 | 108227 | 109530 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| GFPEAKKP_49 | 292 | 1302 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.55 | 99.90 | WP_002986042 |
| GFPEAKKP_56 | 59439 | 60722 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| GFPEAKKP_38 | 7643 | 8689 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFPEAKKP_15 | 23570 | 22587 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GFPEAKKP_58 | 13877 | 12804 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GFPEAKKP_29 | 24544 | 25512 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GFPEAKKP_39 | 15567 | 13264 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| GFPEAKKP_20 | 46208 | 47689 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| GFPEAKKP_4 | 11337 | 9091 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| GFPEAKKP_8 | 10543 | 11535 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| GFPEAKKP_82 | 15301 | 14330 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| GFPEAKKP_39 | 129360 | 130511 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| GFPEAKKP_5 | 35968 | 33980 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| GFPEAKKP_15 | 39767 | 41497 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| GFPEAKKP_36 | 89163 | 87685 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GFPEAKKP_29 | 108227 | 109528 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GFPEAKKP_36 | 73973 | 72081 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| GFPEAKKP_4 | 6166 | 7875 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| GFPEAKKP_59 | 54987 | 53977 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GFPEAKKP_42 | 37740 | 36379 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GFPEAKKP_59 | 4721 | 6613 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| GFPEAKKP_29 | 93791 | 94681 | bglB | 5.29e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| GFPEAKKP_54 | 11820 | 10885 | SP_0676 | 2.27e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| GFPEAKKP_4 | 8899 | 8042 | metF | 1.02e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| GFPEAKKP_89 | 72489 | 73193 | covR | 2.28e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| GFPEAKKP_20 | 27239 | 26568 | rr01 | 1.94e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| GFPEAKKP_89 | 72489 | 73193 | walR | 9.61e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| GFPEAKKP_4 | 2212 | 1544 | CiaR | 1.12e-119 | two-component response regulator | 84.75 | 99.55 | AAK74935 |
| GFPEAKKP_65 | 16139 | 16825 | CovR | 1.29e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GFPEAKKP_4 | 2212 | 1541 | ciaR | 1.21e-117 | two component system response regulator | 82.59 | 99.12 | AIK75708 |
| GFPEAKKP_8 | 11847 | 12242 | SpxA2 | 2.47e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| GFPEAKKP_42 | 49098 | 49499 | SpxA1 | 1.64e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| GFPEAKKP_41 | 13210 | 13497 | spr0479 | 2.96e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| GFPEAKKP_6 | 9933 | 9679 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| GFPEAKKP_36 | 31396 | 31659 | spr0175 | 4.43e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| GFPEAKKP_36 | 32199 | 32489 | spr0177 | 3.22e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| GFPEAKKP_56 | 59281 | 58802 | RopE (M5005_Spy_1611) | 8.51e-72 | probable DNA-directed RNA polymerase subunit delta | 80.62 | 83.77 | AAK34603 |
| GFPEAKKP_39 | 15567 | 13270 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| GFPEAKKP_59 | 19125 | 20144 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFPEAKKP_3 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_4 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_5 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_6 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_7 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_8 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_10 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_11 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_14 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_15 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_16 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_17 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_20 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_29 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_32 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_36 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_37 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_38 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_39 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_40 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_41 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_42 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_43 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_44 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_46 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_47 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_48 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_49 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_50 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_51 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_52 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_53 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_54 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_55 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_56 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_57 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_58 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_59 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_60 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_61 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_62 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_63 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_64 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_65 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_66 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_67 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_68 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_69 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_70 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_71 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_72 | 1.0 | 0.0 | 1.0 |
| GFPEAKKP_74 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_75 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_79 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_80 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_82 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_84 | 0.0 | 1.0 | 0.0 |
| GFPEAKKP_89 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)