safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	37	33	49.58

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
HJEBCPCK_39	5133	5984	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
HJEBCPCK_22	25639	26400	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
HJEBCPCK_22	61164	61757	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
HJEBCPCK_39	16717	18462	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
HJEBCPCK_39	15015	16715	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
HJEBCPCK_39	12989	13795	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
HJEBCPCK_39	12048	12989	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
HJEBCPCK_39	10903	12051	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
HJEBCPCK_3	29527	30645	STU_RS14550	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226029
HJEBCPCK_3	28793	29416	STU_RS14555	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226030
HJEBCPCK_3	26070	27470	STU_RS14565	0.0	Capsule	Immune modulation	100.00	100.00	WP_179970486
HJEBCPCK_3	24582	25574	STU_RS14570	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226034
HJEBCPCK_3	23832	24557	STU_RS14575	0.0	Capsule	Immune modulation	100.00	100.00	WP_041828375
HJEBCPCK_3	19605	20084	STU_RS14610	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226040
HJEBCPCK_3	19017	19502	STU_RS14615	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226041
HJEBCPCK_3	18568	19017	STU_RS14620	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226042
HJEBCPCK_3	17563	18559	STU_RS14625*	0.0	Capsule	Immune modulation	100.00	100.00	-
HJEBCPCK_3	15967	17334	STU_RS14630	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226045
HJEBCPCK_3	14468	15160	STU_RS14640	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226047
HJEBCPCK_3	12273	13727	STU_RS14650	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226049
HJEBCPCK_37	13005	14663	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
HJEBCPCK_39	13795	14997	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
HJEBCPCK_3	27477	28652	STU_RS14560	0.0	Capsule	Immune modulation	99.92	100.00	WP_011226032
HJEBCPCK_3	15170	15910	STU_RS14635	0.0	Capsule	Immune modulation	99.86	100.00	WP_011226046
HJEBCPCK_3	22095	22735	STU_RS14590	0.0	Capsule	Immune modulation	99.84	99.84	WP_041828240
HJEBCPCK_22	61164	61757	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
HJEBCPCK_22	61164	61757	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
HJEBCPCK_3	13728	14459	STU_RS14645	0.0	Capsule	Immune modulation	99.73	100.00	WP_011226048
HJEBCPCK_39	15015	16715	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
HJEBCPCK_39	13795	14997	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
HJEBCPCK_39	12989	13795	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
HJEBCPCK_39	12048	12989	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
HJEBCPCK_3	14468	15160	STR_RS05255	0.0	Capsule	Immune modulation	99.42	100.00	WP_011227247
HJEBCPCK_11	18720	19688	cbpD	0.0	Autolysin	Exoenzyme	99.38	100.00	WP_082302295
HJEBCPCK_8	14292	15082	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
HJEBCPCK_3	30632	31395	STU_RS14545	0.0	Capsule	Immune modulation	99.35	100.53	WP_011226028
HJEBCPCK_39	10903	12051	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
HJEBCPCK_22	60295	61164	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
HJEBCPCK_3	13728	14459	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
HJEBCPCK_3	15170	15910	STER_RS05305	0.0	Capsule	Immune modulation	98.38	100.00	WP_011681174
HJEBCPCK_39	16717	18462	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
HJEBCPCK_3	15170	15905	STR_RS05250	0.0	Capsule	Immune modulation	98.10	98.13	WP_011227246
HJEBCPCK_3	12273	13727	STR_RS05265	0.0	Capsule	Immune modulation	97.94	100.00	WP_041827043
HJEBCPCK_3	13728	14459	STER_RS05315	0.0	Capsule	Immune modulation	97.81	100.00	WP_011681176
HJEBCPCK_39	6105	7364	STER_RS07100	0.0	Capsule	Immune modulation	97.70	100.00	WP_011681445
HJEBCPCK_3	12273	13727	STER_RS05320	0.0	Capsule	Immune modulation	97.47	99.79	WP_011681177
HJEBCPCK_3	15967	17330	STER_RS05300	0.0	Capsule	Immune modulation	94.94	99.71	WP_011681173
HJEBCPCK_3	14468	15160	STER_RS05310	0.0	Capsule	Immune modulation	89.90	100.00	WP_011681175
HJEBCPCK_28	50247	51550	eno	0.0	Streptococcal enolase	Exoenzyme	88.09	99.54	WP_000022832
HJEBCPCK_30	375	1385	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
HJEBCPCK_39	12989	13795	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
HJEBCPCK_33	2349	3632	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
HJEBCPCK_22	62186	63232	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
HJEBCPCK_3	23426	23764	STU_RS19955	2.53e-177	Capsule	Immune modulation	100.00	100.00	WP_148148905
HJEBCPCK_39	7361	8059	STER_RS07095	5.34e-154	Capsule	Immune modulation	81.15	70.35	WP_011681444
HJEBCPCK_39	8413	8879	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
HJEBCPCK_39	13795	14532	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
HJEBCPCK_22	22952	21969	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
HJEBCPCK_77	59249	60322	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
HJEBCPCK_28	133787	132819	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
HJEBCPCK_1	155160	157463	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
HJEBCPCK_90	63540	65021	SPD_0310	0.0	UPF0371 protein SPD_0310	92.11	100.00	ABJ54480
HJEBCPCK_15	11332	9086	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
HJEBCPCK_2	19802	20794	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
HJEBCPCK_88	15302	14331	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
HJEBCPCK_1	41330	40179	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
HJEBCPCK_1	6857	4875	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
HJEBCPCK_22	39146	40876	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
HJEBCPCK_31	26999	28477	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
HJEBCPCK_28	51550	50249	Eno	0.0	enolase	86.21	99.77	ABP90468
HJEBCPCK_31	41735	43627	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.89	98.90	ABP91129
HJEBCPCK_15	6161	7870	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
HJEBCPCK_44	55042	54032	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
HJEBCPCK_19	44710	46071	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
HJEBCPCK_44	4705	6597	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
HJEBCPCK_28	65980	65090	bglB	2.48e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
HJEBCPCK_9	1244	309	SP_0676	1.90e-169	transcription facot	81.73	96.89	AAK74821
HJEBCPCK_15	8894	8037	metF	7.64e-167	methionine biosynthesis enzymes	86.36	99.31	AAK74739
HJEBCPCK_8	42781	43485	covR	1.26e-131	responder protein	84.68	100.00	AAK71319
HJEBCPCK_90	29859	29188	rr01	6.55e-127	response regulator	84.38	99.56	CAB54566
HJEBCPCK_8	42781	43485	walR	9.06e-122	DNA-binding response regulator	80.85	100.00	ANC99504
HJEBCPCK_23	15926	16612	CovR	1.39e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
HJEBCPCK_15	2208	1558	CiaR	1.23e-118	two-component response regulator	86.17	96.88	AAK74935
HJEBCPCK_15	2208	1558	ciaR	6.35e-116	two component system response regulator	83.87	96.02	AIK75708
HJEBCPCK_2	21091	21486	SpxA2	4.30e-76	transcriptional regulator	90.91	100.00	ERL21461
HJEBCPCK_19	33352	32951	SpxA1	1.05e-67	transcriptional regulator	80.60	100.00	ERL19273
HJEBCPCK_14	25275	24988	spr0479	3.35e-49	essential gene	86.46	98.97	AAK99283
HJEBCPCK_11	60575	60829	spr1327	1.05e-45	essential gene	95.29	100.00	AAL00131
HJEBCPCK_31	75379	75116	spr0175	4.30e-42	pathogenitcity related	84.09	100.00	AAK98979
HJEBCPCK_31	74552	74286	spr0177	1.11e-33	essential gene	84.27	88.12	AAK98981
HJEBCPCK_33	3790	4290	RopE (M5005_Spy_1611)	3.53e-76	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
HJEBCPCK_1	155160	157457	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
HJEBCPCK_44	19166	20185	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
HJEBCPCK_1	0.0	1.0	0.0
HJEBCPCK_2	0.0	1.0	0.0
HJEBCPCK_3	0.0	1.0	0.0
HJEBCPCK_4	0.0	1.0	0.0
HJEBCPCK_5	0.0	1.0	0.0
HJEBCPCK_6	0.0	1.0	0.0
HJEBCPCK_7	0.0	1.0	0.0
HJEBCPCK_8	0.0	1.0	0.0
HJEBCPCK_9	0.0	1.0	0.0
HJEBCPCK_10	0.0	1.0	0.0
HJEBCPCK_11	0.0	1.0	0.0
HJEBCPCK_13	0.0	1.0	0.0
HJEBCPCK_14	0.0	1.0	0.0
HJEBCPCK_15	0.0	1.0	0.0
HJEBCPCK_16	0.0	1.0	0.0
HJEBCPCK_17	0.0	1.0	0.0
HJEBCPCK_18	0.0	1.0	0.0
HJEBCPCK_19	0.0	1.0	0.0
HJEBCPCK_21	0.0	1.0	0.0
HJEBCPCK_22	0.0	1.0	0.0
HJEBCPCK_23	0.0	1.0	0.0
HJEBCPCK_24	0.0	1.0	0.0
HJEBCPCK_25	0.0	1.0	0.0
HJEBCPCK_26	0.0	1.0	0.0
HJEBCPCK_28	0.0	1.0	0.0
HJEBCPCK_29	0.0	1.0	0.0
HJEBCPCK_30	0.0	1.0	0.0
HJEBCPCK_31	0.0	1.0	0.0
HJEBCPCK_32	0.0	1.0	0.0
HJEBCPCK_33	0.0	1.0	0.0
HJEBCPCK_34	0.0	1.0	0.0
HJEBCPCK_35	0.0	1.0	0.0
HJEBCPCK_37	0.0	1.0	0.0
HJEBCPCK_39	0.0	1.0	0.0
HJEBCPCK_41	0.0	1.0	0.0
HJEBCPCK_43	0.0	1.0	0.0
HJEBCPCK_44	0.0	1.0	0.0
HJEBCPCK_45	0.0	1.0	0.0
HJEBCPCK_46	1.0	0.0	1.0
HJEBCPCK_47	0.0	1.0	0.0
HJEBCPCK_48	0.0	1.0	0.0
HJEBCPCK_49	0.0	1.0	0.0
HJEBCPCK_53	1.0	0.0	1.0
HJEBCPCK_56	0.0	1.0	0.0
HJEBCPCK_57	1.0	0.0	1.0
HJEBCPCK_59	0.0	1.0	0.0
HJEBCPCK_60	0.0	1.0	0.0
HJEBCPCK_62	0.0	1.0	0.0
HJEBCPCK_65	0.0	1.0	0.0
HJEBCPCK_66	0.0	1.0	0.0
HJEBCPCK_77	0.0	1.0	0.0
HJEBCPCK_88	0.0	1.0	0.0
HJEBCPCK_90	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found