Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 18 | 31 | 35.85 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MOPIOGHA_17 | 1205961 | 1206830 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| MOPIOGHA_17 | 1206830 | 1207423 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| MOPIOGHA_17 | 1206830 | 1207423 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| MOPIOGHA_17 | 1538646 | 1539905 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| MOPIOGHA_17 | 1206830 | 1207423 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| MOPIOGHA_17 | 1479355 | 1480194 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| MOPIOGHA_17 | 1536686 | 1537654 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011681443 |
| MOPIOGHA_17 | 1319901 | 1321559 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| MOPIOGHA_17 | 1537672 | 1538649 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | WP_011681444 |
| MOPIOGHA_17 | 236482 | 237270 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.36 | 99.75 | - |
| MOPIOGHA_17 | 1535969 | 1536684 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.18 | 99.86 | WP_011681442 |
| MOPIOGHA_17 | 1534847 | 1535623 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681440 |
| MOPIOGHA_17 | 1169876 | 1170637 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| MOPIOGHA_17 | 1540024 | 1540875 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| MOPIOGHA_17 | 188475 | 189934 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.99 | 100.14 | WP_011226049 |
| MOPIOGHA_17 | 188475 | 189934 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.85 | 99.93 | WP_011681177 |
| MOPIOGHA_17 | 188475 | 189934 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.69 | 100.14 | WP_041827043 |
| MOPIOGHA_17 | 439357 | 440660 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| MOPIOGHA_17 | 1731984 | 1732989 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.48 | 98.91 | WP_002986042 |
| MOPIOGHA_17 | 1207852 | 1208898 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| MOPIOGHA_17 | 1466403 | 1467686 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| MOPIOGHA_17 | 192318 | 192901 | SPD_RS01775 | 1.05e-151 | Capsule | Immune modulation | 83.59 | 96.20 | WP_001819836 |
| MOPIOGHA_17 | 189935 | 190399 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 96.34 | 63.52 | WP_011681176 |
| MOPIOGHA_17 | 189935 | 190399 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 96.13 | 63.52 | WP_011226048 |
| MOPIOGHA_17 | 189935 | 190399 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 95.91 | 63.52 | WP_011227248 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MOPIOGHA_17 | 1167189 | 1166206 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| MOPIOGHA_17 | 1517881 | 1516808 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| MOPIOGHA_17 | 1704342 | 1706645 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| MOPIOGHA_17 | 524061 | 523093 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| MOPIOGHA_17 | 1025033 | 1026514 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| MOPIOGHA_17 | 629052 | 626806 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| MOPIOGHA_17 | 1484549 | 1483557 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| MOPIOGHA_17 | 22721 | 21750 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| MOPIOGHA_17 | 1590555 | 1589404 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| MOPIOGHA_17 | 1554124 | 1552136 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| MOPIOGHA_17 | 1183386 | 1185116 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| MOPIOGHA_12 | 80735 | 79257 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| MOPIOGHA_17 | 440660 | 439359 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| MOPIOGHA_12 | 65546 | 63654 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| MOPIOGHA_17 | 623881 | 625590 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| MOPIOGHA_17 | 157007 | 155997 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| MOPIOGHA_17 | 1343617 | 1342256 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| MOPIOGHA_17 | 106737 | 108629 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| MOPIOGHA_17 | 455095 | 454205 | bglB | 5.08e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| MOPIOGHA_17 | 693523 | 694458 | SP_0676 | 7.14e-168 | transcription facot | 81.73 | 96.89 | AAK74821 |
| MOPIOGHA_17 | 626614 | 625757 | metF | 1.48e-164 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| MOPIOGHA_17 | 208802 | 208098 | covR | 4.24e-131 | responder protein | 85.53 | 100.00 | AAK71319 |
| MOPIOGHA_17 | 1006058 | 1005387 | rr01 | 2.91e-124 | response regulator | 83.93 | 99.56 | CAB54566 |
| MOPIOGHA_17 | 208802 | 208098 | walR | 1.78e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| MOPIOGHA_17 | 85963 | 85277 | CovR | 9.73e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| MOPIOGHA_17 | 619927 | 619259 | CiaR | 1.95e-117 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| MOPIOGHA_17 | 619927 | 619256 | ciaR | 1.92e-115 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| MOPIOGHA_17 | 1483245 | 1482850 | SpxA2 | 3.58e-74 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| MOPIOGHA_17 | 1354976 | 1355377 | SpxA1 | 3.64e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| MOPIOGHA_12 | 31902 | 32165 | spr0175 | 4.15e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| MOPIOGHA_12 | 32729 | 32995 | spr0177 | 9.09e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| MOPIOGHA_17 | 1466245 | 1465745 | RopE (M5005_Spy_1611) | 4.76e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| MOPIOGHA_17 | 1704342 | 1706639 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| MOPIOGHA_17 | 121145 | 122164 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MOPIOGHA_1 | 1.0 | 0.0 | 1.0 |
| MOPIOGHA_5 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_6 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_8 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_10 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_11 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_12 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_15 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_17 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_19 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_21 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_22 | 0.0 | 1.0 | 0.0 |
| MOPIOGHA_24 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MOPIOGHA_17 | 312710 | 439296 | False | Siphoviridae | VOG3299, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG6163, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG3424, VOG0054, VOG10230, VOG3299, VOG3300, VOG3303, VOG0275, VOG7656, VOG4602, VOG8520, VOG5353, VOG1564, VOG9469, VOG0651, VOG4566, VOG0189, VOG3430, VOG0283, VOG0685, VOG7936, VOG0226, VOG10904, VOG4693, VOG0655, VOG6414, VOG0656, VOG9667, VOG0657, VOG0658, VOG0198, VOG3300, VOG3299, VOG10230, VOG0054, VOG3424, VOG3298, VOG4782, VOG0647, VOG4599, VOG4605, VOG6163, VOG0800, VOG0725, VOG0799, VOG0724, VOG5660, VOG0723, VOG4713, VOG3435, VOG1887, VOG3434, VOG4555, VOG4564, VOG0720, VOG4544, VOG0796, VOG3299, VOG3300, VOG0198, VOG0658, VOG0657, VOG9667, VOG0656, VOG6414, VOG0655, VOG4693, VOG10904, VOG0226, VOG7936, VOG0685, VOG0283, VOG3430, VOG0189, VOG4566, VOG0651, VOG9469, VOG1564, VOG5353, VOG8520, VOG4602, VOG7656, VOG0275, VOG3303, VOG3300, VOG3299, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG6163, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG3424, VOG0054, VOG10230, VOG3299, VOG3300, VOG0198, VOG0658, VOG0657, VOG9667, VOG0656, VOG6414, VOG0655, VOG4693, VOG10904, VOG0226, VOG7936, VOG0685, VOG0283, VOG3430, VOG0189, VOG4566, VOG0651, VOG9469, VOG1564, VOG5353, VOG8520, VOG4602, VOG7656 |
Phigaro v2.4.0 (Database: Jan. 2024 release)