Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HNGJNMJB_56 | 19476 | 20345 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| HNGJNMJB_56 | 20345 | 20938 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| HNGJNMJB_53 | 25501 | 26760 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| HNGJNMJB_56 | 20345 | 20938 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| HNGJNMJB_56 | 20345 | 20938 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| HNGJNMJB_53 | 26757 | 27733 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| HNGJNMJB_17 | 5774 | 7432 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| HNGJNMJB_53 | 27751 | 28719 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| HNGJNMJB_104 | 14618 | 15379 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| HNGJNMJB_108 | 3897 | 4628 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011226048 |
| HNGJNMJB_108 | 3897 | 4628 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011227248 |
| HNGJNMJB_108 | 3897 | 4628 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| HNGJNMJB_53 | 28721 | 29436 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| HNGJNMJB_114 | 21638 | 22433 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 97.99 | 100.51 | - |
| HNGJNMJB_53 | 29782 | 30552 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.92 | 99.23 | WP_011681440 |
| HNGJNMJB_74 | 269 | 1684 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| HNGJNMJB_108 | 4629 | 6089 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| HNGJNMJB_106 | 18974 | 19933 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| HNGJNMJB_53 | 24531 | 25382 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| HNGJNMJB_108 | 3196 | 3888 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.11 | 100.00 | WP_011226047 |
| HNGJNMJB_108 | 3196 | 3888 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011227247 |
| HNGJNMJB_108 | 4629 | 6089 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_011226049 |
| HNGJNMJB_108 | 4629 | 6089 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| HNGJNMJB_108 | 1006 | 2371 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.46 | 99.85 | WP_011681173 |
| HNGJNMJB_108 | 1004 | 2371 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| HNGJNMJB_108 | 2428 | 3186 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.41 | 100.94 | WP_011681174 |
| HNGJNMJB_108 | 2428 | 3186 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.02 | 100.94 | WP_011226046 |
| HNGJNMJB_108 | 2433 | 3186 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.71 | 99.07 | WP_011227246 |
| HNGJNMJB_108 | 3196 | 3888 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.06 | 99.86 | WP_011681175 |
| HNGJNMJB_47 | 28037 | 29340 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| HNGJNMJB_9 | 14330 | 15340 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| HNGJNMJB_56 | 21367 | 22413 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| HNGJNMJB_107 | 59474 | 60757 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.50 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HNGJNMJB_104 | 18066 | 19049 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| HNGJNMJB_7 | 13760 | 12687 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| HNGJNMJB_43 | 138034 | 140337 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| HNGJNMJB_47 | 112942 | 111974 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| HNGJNMJB_57 | 46180 | 47661 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| HNGJNMJB_5 | 11363 | 9117 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| HNGJNMJB_65 | 1903 | 911 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| HNGJNMJB_63 | 117 | 1088 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| HNGJNMJB_43 | 24224 | 23073 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| HNGJNMJB_53 | 11493 | 13481 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| HNGJNMJB_104 | 1868 | 138 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| HNGJNMJB_54 | 80211 | 78733 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| HNGJNMJB_47 | 29340 | 28039 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| HNGJNMJB_54 | 65022 | 63130 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| HNGJNMJB_5 | 6192 | 7901 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| HNGJNMJB_66 | 18816 | 19826 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| HNGJNMJB_13 | 30236 | 31597 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| HNGJNMJB_50 | 48606 | 46714 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| HNGJNMJB_47 | 43775 | 42885 | bglB | 3.53e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| HNGJNMJB_110 | 10010 | 9075 | SP_0676 | 1.94e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| HNGJNMJB_5 | 8925 | 8068 | metF | 1.35e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| HNGJNMJB_67 | 16544 | 17248 | covR | 9.58e-133 | responder protein | 85.53 | 100.00 | AAK71319 |
| HNGJNMJB_57 | 27205 | 26534 | rr01 | 1.94e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| HNGJNMJB_67 | 16544 | 17248 | walR | 4.04e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| HNGJNMJB_105 | 16137 | 16823 | CovR | 1.30e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| HNGJNMJB_5 | 2238 | 1570 | CiaR | 1.80e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| HNGJNMJB_5 | 2238 | 1567 | ciaR | 1.77e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| HNGJNMJB_65 | 614 | 240 | SpxA2 | 8.92e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| HNGJNMJB_13 | 18872 | 18471 | SpxA1 | 1.97e-62 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| HNGJNMJB_86 | 13684 | 13971 | spr0479 | 3.00e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| HNGJNMJB_106 | 60821 | 61075 | spr1327 | 6.90e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| HNGJNMJB_54 | 31393 | 31656 | spr0175 | 4.13e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| HNGJNMJB_54 | 32220 | 32486 | spr0177 | 9.03e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| HNGJNMJB_107 | 59316 | 58816 | RopE (M5005_Spy_1611) | 1.67e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| HNGJNMJB_43 | 138034 | 140331 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| HNGJNMJB_50 | 34132 | 33113 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HNGJNMJB_1 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_2 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_3 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_4 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_5 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_6 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_7 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_8 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_9 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_10 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_12 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_13 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_14 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_15 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_16 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_17 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_18 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_20 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_22 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_23 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_24 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_25 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_26 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_28 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_29 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_31 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_33 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_35 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_43 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_47 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_49 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_50 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_52 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_53 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_54 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_55 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_56 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_57 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_58 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_59 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_60 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_62 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_63 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_64 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_65 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_66 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_67 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_68 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_69 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_71 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_74 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_75 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_77 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_79 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_85 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_86 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_87 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_89 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_96 | 1.0 | 0.0 | 1.0 |
| HNGJNMJB_101 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_104 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_105 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_106 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_107 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_108 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_109 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_110 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_111 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_113 | 0.0 | 1.0 | 0.0 |
| HNGJNMJB_114 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)