Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 33 | 42.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| APPENMAO_77 | 31917 | 32510 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| APPENMAO_7 | 74181 | 75137 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| APPENMAO_7 | 75121 | 75930 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| APPENMAO_77 | 31917 | 32510 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| APPENMAO_77 | 31917 | 32510 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| APPENMAO_7 | 73036 | 74184 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| APPENMAO_7 | 68689 | 69540 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| APPENMAO_7 | 77155 | 78210 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_002948022 |
| APPENMAO_7 | 75930 | 77135 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226326 |
| APPENMAO_34 | 321 | 1979 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| APPENMAO_7 | 78207 | 79991 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| APPENMAO_9 | 13960 | 14559 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_196767671 |
| APPENMAO_77 | 31048 | 31917 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| APPENMAO_55 | 571 | 1986 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.01 | 100.00 | WP_011681165 |
| APPENMAO_77 | 76246 | 77036 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| APPENMAO_7 | 71917 | 72927 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011226330 |
| APPENMAO_1 | 25631 | 26392 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| APPENMAO_21 | 18850 | 19818 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.76 | 100.00 | WP_082302295 |
| APPENMAO_9 | 16895 | 18349 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.76 | 100.00 | WP_011226049 |
| APPENMAO_9 | 16895 | 18349 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.62 | 100.00 | WP_041827043 |
| APPENMAO_9 | 12836 | 13795 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_041827042 |
| APPENMAO_7 | 70643 | 71917 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.20 | 100.00 | WP_011226331 |
| APPENMAO_9 | 14711 | 15446 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.96 | 99.33 | WP_011681174 |
| APPENMAO_9 | 14711 | 15446 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.83 | 99.33 | WP_011226046 |
| APPENMAO_9 | 16163 | 16894 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011681176 |
| APPENMAO_9 | 14697 | 15446 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.47 | 100.00 | WP_011227246 |
| APPENMAO_9 | 16163 | 16894 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.27 | 100.00 | WP_011226048 |
| APPENMAO_9 | 16895 | 18349 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 99.79 | WP_011681177 |
| APPENMAO_9 | 16163 | 16894 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.86 | 100.00 | WP_011227248 |
| APPENMAO_9 | 15456 | 16137 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 93.25 | 98.41 | WP_011226047 |
| APPENMAO_9 | 15456 | 16137 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 92.96 | 98.41 | WP_011227247 |
| APPENMAO_10 | 106769 | 108072 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.86 | 99.54 | WP_000022832 |
| APPENMAO_27 | 270 | 1280 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| APPENMAO_9 | 15456 | 16137 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 85.92 | 98.41 | WP_011681175 |
| APPENMAO_18 | 62914 | 64197 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| APPENMAO_77 | 32939 | 33985 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| APPENMAO_1 | 22944 | 21961 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| APPENMAO_10 | 208729 | 209802 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| APPENMAO_40 | 40709 | 43012 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| APPENMAO_10 | 24559 | 25527 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| APPENMAO_64 | 48422 | 49903 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| APPENMAO_11 | 11334 | 9088 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| APPENMAO_75 | 10432 | 11430 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| APPENMAO_33 | 136 | 1107 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 89.81 | 100.00 | ABF36968 |
| APPENMAO_7 | 25713 | 26864 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| APPENMAO_7 | 53719 | 55707 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| APPENMAO_77 | 9900 | 11630 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| APPENMAO_4 | 27061 | 28539 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| APPENMAO_10 | 106769 | 108070 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| APPENMAO_4 | 42256 | 44148 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| APPENMAO_11 | 6162 | 7871 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| APPENMAO_13 | 7269 | 8279 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| APPENMAO_32 | 43857 | 45218 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| APPENMAO_13 | 57536 | 55644 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| APPENMAO_10 | 92337 | 93227 | bglB | 3.49e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| APPENMAO_42 | 13343 | 12405 | SP_0676 | 2.41e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| APPENMAO_11 | 8896 | 8039 | metF | 2.02e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| APPENMAO_77 | 104668 | 105372 | covR | 4.72e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| APPENMAO_64 | 29452 | 28781 | rr01 | 1.72e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| APPENMAO_77 | 104668 | 105372 | walR | 2.23e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| APPENMAO_11 | 2208 | 1540 | CiaR | 5.35e-122 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| APPENMAO_11 | 2208 | 1537 | ciaR | 7.61e-120 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| APPENMAO_36 | 10233 | 9547 | CovR | 1.27e-118 | serves to repress GAS virulence factor-encoding genes | 80.35 | 100.00 | ACE75886 |
| APPENMAO_75 | 11706 | 12080 | SpxA2 | 7.56e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| APPENMAO_32 | 32500 | 32099 | SpxA1 | 1.03e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| APPENMAO_38 | 14808 | 15095 | spr0479 | 3.07e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| APPENMAO_21 | 60705 | 60959 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| APPENMAO_4 | 75794 | 75531 | spr0175 | 4.31e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| APPENMAO_4 | 74991 | 74701 | spr0177 | 2.53e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| APPENMAO_18 | 62756 | 62256 | RopE (M5005_Spy_1611) | 1.76e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| APPENMAO_40 | 40709 | 43006 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| APPENMAO_13 | 43136 | 42117 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| APPENMAO_1 | 0.0 | 1.0 | 0.0 |
| APPENMAO_2 | 0.0 | 1.0 | 0.0 |
| APPENMAO_3 | 0.0 | 1.0 | 0.0 |
| APPENMAO_4 | 0.0 | 1.0 | 0.0 |
| APPENMAO_7 | 0.0 | 1.0 | 0.0 |
| APPENMAO_8 | 0.0 | 1.0 | 0.0 |
| APPENMAO_9 | 0.0 | 1.0 | 0.0 |
| APPENMAO_10 | 0.0 | 1.0 | 0.0 |
| APPENMAO_11 | 0.0 | 1.0 | 0.0 |
| APPENMAO_12 | 0.0 | 1.0 | 0.0 |
| APPENMAO_13 | 0.0 | 1.0 | 0.0 |
| APPENMAO_14 | 0.0 | 1.0 | 0.0 |
| APPENMAO_15 | 0.0 | 1.0 | 0.0 |
| APPENMAO_16 | 0.0 | 1.0 | 0.0 |
| APPENMAO_17 | 0.0 | 1.0 | 0.0 |
| APPENMAO_18 | 0.0 | 1.0 | 0.0 |
| APPENMAO_19 | 0.0 | 1.0 | 0.0 |
| APPENMAO_20 | 0.0 | 1.0 | 0.0 |
| APPENMAO_21 | 0.0 | 1.0 | 0.0 |
| APPENMAO_23 | 0.0 | 1.0 | 0.0 |
| APPENMAO_24 | 1.0 | 0.0 | 1.0 |
| APPENMAO_25 | 0.0 | 1.0 | 0.0 |
| APPENMAO_26 | 0.0 | 1.0 | 0.0 |
| APPENMAO_27 | 0.0 | 1.0 | 0.0 |
| APPENMAO_28 | 0.0 | 1.0 | 0.0 |
| APPENMAO_31 | 1.0 | 0.0 | 1.0 |
| APPENMAO_32 | 0.0 | 1.0 | 0.0 |
| APPENMAO_33 | 0.0 | 1.0 | 0.0 |
| APPENMAO_34 | 0.0 | 1.0 | 0.0 |
| APPENMAO_35 | 0.0 | 1.0 | 0.0 |
| APPENMAO_36 | 0.0 | 1.0 | 0.0 |
| APPENMAO_37 | 0.0 | 1.0 | 0.0 |
| APPENMAO_38 | 0.0 | 1.0 | 0.0 |
| APPENMAO_39 | 0.0 | 1.0 | 0.0 |
| APPENMAO_40 | 0.0 | 1.0 | 0.0 |
| APPENMAO_42 | 0.0 | 1.0 | 0.0 |
| APPENMAO_43 | 0.0 | 1.0 | 0.0 |
| APPENMAO_45 | 0.0 | 1.0 | 0.0 |
| APPENMAO_46 | 1.0 | 0.0 | 1.0 |
| APPENMAO_47 | 0.0 | 1.0 | 0.0 |
| APPENMAO_49 | 0.0 | 1.0 | 0.0 |
| APPENMAO_50 | 1.0 | 0.0 | 1.0 |
| APPENMAO_51 | 0.0 | 1.0 | 0.0 |
| APPENMAO_52 | 0.0 | 1.0 | 0.0 |
| APPENMAO_53 | 0.0 | 1.0 | 0.0 |
| APPENMAO_54 | 0.0 | 1.0 | 0.0 |
| APPENMAO_55 | 1.0 | 0.0 | 1.0 |
| APPENMAO_57 | 0.0 | 1.0 | 0.0 |
| APPENMAO_61 | 0.0 | 1.0 | 0.0 |
| APPENMAO_64 | 0.0 | 1.0 | 0.0 |
| APPENMAO_75 | 0.0 | 1.0 | 0.0 |
| APPENMAO_77 | 0.0 | 1.0 | 0.0 |
| APPENMAO_78 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)