Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LIFKMMNK_49 | 9587 | 10456 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| LIFKMMNK_49 | 8994 | 9587 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| LIFKMMNK_56 | 29848 | 31107 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| LIFKMMNK_49 | 8994 | 9587 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LIFKMMNK_49 | 8994 | 9587 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| LIFKMMNK_56 | 28875 | 29851 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| LIFKMMNK_12 | 1390 | 3048 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| LIFKMMNK_56 | 27889 | 28857 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| LIFKMMNK_50 | 14616 | 15377 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| LIFKMMNK_105 | 3895 | 4626 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011226048 |
| LIFKMMNK_105 | 3895 | 4626 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011227248 |
| LIFKMMNK_105 | 3895 | 4626 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| LIFKMMNK_56 | 27172 | 27887 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| LIFKMMNK_56 | 26056 | 26826 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.92 | 99.23 | WP_011681440 |
| LIFKMMNK_67 | 414 | 1829 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| LIFKMMNK_105 | 4627 | 6087 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| LIFKMMNK_101 | 18881 | 19840 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| LIFKMMNK_46 | 814 | 1612 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 97.62 | 100.88 | - |
| LIFKMMNK_56 | 31226 | 32077 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| LIFKMMNK_105 | 3194 | 3886 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.11 | 100.00 | WP_011226047 |
| LIFKMMNK_105 | 3194 | 3886 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011227247 |
| LIFKMMNK_105 | 4627 | 6087 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_011226049 |
| LIFKMMNK_105 | 4627 | 6087 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| LIFKMMNK_105 | 1004 | 2369 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.46 | 99.85 | WP_011681173 |
| LIFKMMNK_105 | 1002 | 2369 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| LIFKMMNK_105 | 2426 | 3184 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.41 | 100.94 | WP_011681174 |
| LIFKMMNK_105 | 2426 | 3184 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.02 | 100.94 | WP_011226046 |
| LIFKMMNK_105 | 2431 | 3184 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.71 | 99.07 | WP_011227246 |
| LIFKMMNK_105 | 3194 | 3886 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.06 | 99.86 | WP_011681175 |
| LIFKMMNK_116 | 28166 | 29469 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| LIFKMMNK_89 | 90 | 1100 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| LIFKMMNK_49 | 7519 | 8565 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| LIFKMMNK_11 | 2305 | 3588 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LIFKMMNK_50 | 18064 | 19047 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| LIFKMMNK_4 | 13756 | 12683 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| LIFKMMNK_72 | 40714 | 43017 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| LIFKMMNK_116 | 113071 | 112103 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| LIFKMMNK_94 | 74039 | 72558 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| LIFKMMNK_6 | 11359 | 9113 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| LIFKMMNK_29 | 1897 | 905 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| LIFKMMNK_104 | 23 | 994 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| LIFKMMNK_108 | 21066 | 19915 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| LIFKMMNK_56 | 45115 | 43127 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| LIFKMMNK_50 | 1866 | 136 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| LIFKMMNK_14 | 26987 | 28465 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| LIFKMMNK_116 | 29469 | 28168 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| LIFKMMNK_14 | 42176 | 44068 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| LIFKMMNK_6 | 6188 | 7897 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| LIFKMMNK_96 | 18814 | 19824 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| LIFKMMNK_51 | 1660 | 299 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| LIFKMMNK_98 | 50041 | 48149 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| LIFKMMNK_116 | 43904 | 43014 | bglB | 3.53e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| LIFKMMNK_110 | 10009 | 9074 | SP_0676 | 1.94e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| LIFKMMNK_6 | 8921 | 8064 | metF | 2.18e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| LIFKMMNK_111 | 16540 | 17244 | covR | 9.58e-133 | responder protein | 85.53 | 100.00 | AAK71319 |
| LIFKMMNK_94 | 93014 | 93685 | rr01 | 1.94e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| LIFKMMNK_111 | 16540 | 17244 | walR | 4.04e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| LIFKMMNK_16 | 16135 | 16821 | CovR | 1.30e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| LIFKMMNK_6 | 2234 | 1566 | CiaR | 2.89e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| LIFKMMNK_6 | 2234 | 1563 | ciaR | 2.84e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| LIFKMMNK_29 | 608 | 234 | SpxA2 | 8.91e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| LIFKMMNK_51 | 13024 | 13425 | SpxA1 | 6.44e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| LIFKMMNK_80 | 25489 | 25202 | spr0479 | 2.99e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| LIFKMMNK_101 | 60728 | 60982 | spr1327 | 6.89e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| LIFKMMNK_14 | 75805 | 75542 | spr0175 | 4.13e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| LIFKMMNK_14 | 74978 | 74712 | spr0177 | 9.03e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| LIFKMMNK_11 | 3746 | 4246 | RopE (M5005_Spy_1611) | 1.67e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| LIFKMMNK_72 | 40714 | 43011 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| LIFKMMNK_98 | 35567 | 34548 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LIFKMMNK_2 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_3 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_4 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_5 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_6 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_7 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_8 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_9 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_10 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_11 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_12 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_14 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_15 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_16 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_20 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_22 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_25 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_26 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_29 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_39 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_40 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_41 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_42 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_43 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_44 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_45 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_46 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_47 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_48 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_49 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_50 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_51 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_52 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_53 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_54 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_55 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_56 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_59 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_60 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_61 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_62 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_66 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_67 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_70 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_72 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_73 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_80 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_89 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_91 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_93 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_94 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_95 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_96 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_97 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_98 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_99 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_100 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_101 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_102 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_103 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_104 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_105 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_106 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_107 | 1.0 | 0.0 | 1.0 |
| LIFKMMNK_108 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_109 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_110 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_111 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_112 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_113 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_115 | 0.0 | 1.0 | 0.0 |
| LIFKMMNK_116 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)