Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EKMEGDEP_26 | 2169 | 3038 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| EKMEGDEP_26 | 1576 | 2169 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| EKMEGDEP_26 | 1576 | 2169 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| EKMEGDEP_102 | 19391 | 20650 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.76 | 100.00 | WP_011681445 |
| EKMEGDEP_26 | 1576 | 2169 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| EKMEGDEP_29 | 4567 | 6225 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| EKMEGDEP_17 | 9201 | 10040 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| EKMEGDEP_102 | 18418 | 19394 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 99.90 | WP_011681444 |
| EKMEGDEP_79 | 44035 | 44825 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.24 | 100.00 | - |
| EKMEGDEP_102 | 17432 | 18400 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011681443 |
| EKMEGDEP_55 | 14622 | 15383 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| EKMEGDEP_103 | 3679 | 4410 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| EKMEGDEP_115 | 1175 | 2590 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | WP_011681165 |
| EKMEGDEP_103 | 3679 | 4410 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| EKMEGDEP_103 | 3679 | 4410 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| EKMEGDEP_102 | 16715 | 17430 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.05 | 99.86 | WP_011681442 |
| EKMEGDEP_103 | 2978 | 3670 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011227247 |
| EKMEGDEP_103 | 2978 | 3670 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.83 | 100.00 | WP_011226047 |
| EKMEGDEP_103 | 4411 | 5871 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681177 |
| EKMEGDEP_102 | 15599 | 16369 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.15 | 99.10 | WP_011681440 |
| EKMEGDEP_103 | 2230 | 2968 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.02 | 99.73 | WP_011681174 |
| EKMEGDEP_103 | 4411 | 5871 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| EKMEGDEP_103 | 2233 | 2968 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| EKMEGDEP_102 | 20769 | 21620 | rfbD | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_014621820 |
| EKMEGDEP_103 | 2230 | 2968 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.35 | 99.73 | WP_011226046 |
| EKMEGDEP_103 | 4411 | 5871 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| EKMEGDEP_103 | 803 | 2170 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.96 | 100.00 | WP_011226045 |
| EKMEGDEP_103 | 805 | 2170 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.36 | 99.85 | WP_011681173 |
| EKMEGDEP_103 | 2978 | 3670 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.04 | 100.00 | WP_011681175 |
| EKMEGDEP_121 | 28044 | 29347 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| EKMEGDEP_12 | 13452 | 14462 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.86 | 99.90 | WP_002986042 |
| EKMEGDEP_89 | 2907 | 4190 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| EKMEGDEP_26 | 101 | 1147 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EKMEGDEP_55 | 18070 | 19053 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EKMEGDEP_15 | 9076 | 8003 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| EKMEGDEP_96 | 51738 | 50770 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| EKMEGDEP_100 | 15567 | 13264 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EKMEGDEP_101 | 1680 | 199 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| EKMEGDEP_108 | 8712 | 10958 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EKMEGDEP_59 | 1917 | 925 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| EKMEGDEP_9 | 119 | 1090 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| EKMEGDEP_95 | 21345 | 22496 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| EKMEGDEP_102 | 34870 | 32882 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| EKMEGDEP_55 | 1870 | 140 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| EKMEGDEP_112 | 27026 | 28504 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| EKMEGDEP_121 | 29347 | 28046 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| EKMEGDEP_112 | 42215 | 44107 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| EKMEGDEP_108 | 13883 | 12174 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| EKMEGDEP_18 | 34929 | 33919 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EKMEGDEP_23 | 1524 | 163 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EKMEGDEP_81 | 4720 | 6612 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| EKMEGDEP_121 | 43777 | 42887 | bglB | 3.59e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| EKMEGDEP_13 | 13457 | 14395 | SP_0676 | 2.89e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| EKMEGDEP_108 | 11150 | 12007 | metF | 4.89e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| EKMEGDEP_79 | 72505 | 73209 | covR | 2.28e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| EKMEGDEP_75 | 24314 | 23643 | rr01 | 6.59e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| EKMEGDEP_79 | 72505 | 73209 | walR | 9.61e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EKMEGDEP_21 | 8002 | 7316 | CovR | 1.29e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| EKMEGDEP_108 | 17840 | 18508 | CiaR | 2.14e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| EKMEGDEP_108 | 17840 | 18511 | ciaR | 2.11e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| EKMEGDEP_59 | 613 | 218 | SpxA2 | 2.45e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| EKMEGDEP_23 | 12881 | 13282 | SpxA1 | 6.41e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| EKMEGDEP_58 | 13208 | 13495 | spr0479 | 2.95e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| EKMEGDEP_104 | 9893 | 9639 | spr1327 | 4.22e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| EKMEGDEP_82 | 17548 | 17285 | spr0175 | 1.27e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| EKMEGDEP_82 | 16721 | 16455 | spr0177 | 2.78e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| EKMEGDEP_89 | 4348 | 4848 | RopE (M5005_Spy_1611) | 7.90e-77 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EKMEGDEP_100 | 15567 | 13270 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EKMEGDEP_81 | 19124 | 20143 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 95.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EKMEGDEP_2 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_6 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_8 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_9 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_10 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_11 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_12 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_13 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_14 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_15 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_16 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_17 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_18 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_19 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_20 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_21 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_22 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_23 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_24 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_25 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_26 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_27 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_29 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_30 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_31 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_33 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_34 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_36 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_37 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_38 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_39 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_40 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_42 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_43 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_44 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_45 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_46 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_47 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_49 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_50 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_51 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_53 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_54 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_55 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_56 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_58 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_59 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_64 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_68 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_73 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_75 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_77 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_78 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_79 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_81 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_82 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_84 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_85 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_86 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_87 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_88 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_89 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_90 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_91 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_92 | 1.0 | 0.0 | 1.0 |
| EKMEGDEP_93 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_95 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_96 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_97 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_99 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_100 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_101 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_102 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_103 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_104 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_105 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_107 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_108 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_110 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_112 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_113 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_114 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_115 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_117 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_120 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_121 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_123 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_127 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_130 | 0.0 | 1.0 | 0.0 |
| EKMEGDEP_131 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)