Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EJANPMFG_4 | 3912347 | 3913780 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| EJANPMFG_4 | 3862487 | 3863080 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| EJANPMFG_4 | 3614418 | 3616064 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| EJANPMFG_4 | 3225406 | 3227976 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| EJANPMFG_4 | 2757940 | 2758278 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EJANPMFG_4 | 2757623 | 2757940 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EJANPMFG_4 | 1730817 | 1731986 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EJANPMFG_4 | 1557524 | 1558726 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EJANPMFG_4 | 3925673 | 3926592 | mph(K) | Spiramycin, Telithromycin | 97.72 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EJANPMFG_4 | 3614418 | 3616061 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| EJANPMFG_4 | 30426 | 30944 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| EJANPMFG_4 | 2165613 | 2168210 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EJANPMFG_4 | 520937 | 521386 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| EJANPMFG_4 | 519741 | 520922 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| EJANPMFG_4 | 1937090 | 1937731 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| EJANPMFG_4 | 1006929 | 1007714 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| EJANPMFG_4 | 521405 | 522547 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| EJANPMFG_4 | 2212320 | 2214083 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| EJANPMFG_4 | 1010601 | 1011539 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| EJANPMFG_4 | 1007740 | 1008936 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| EJANPMFG_4 | 1011559 | 1018695 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.57 | 100.00 | NP_391076 |
| EJANPMFG_4 | 1008954 | 1010573 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EJANPMFG_6 | 29057 | 30193 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| EJANPMFG_4 | 4110970 | 4110101 | pdxS | 3.07e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EJANPMFG_4 | 2443299 | 2442523 | CodY | 3.35e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EJANPMFG_4 | 677872 | 677303 | ClpP | 2.48e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| EJANPMFG_2 | 3888 | 4081 | Col(SD853) | 194 / 194 | 97.42 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EJANPMFG_1 | 1.0 | 0.0 | 1.0 |
| EJANPMFG_2 | 1.0 | 0.0 | 1.0 |
| EJANPMFG_3 | 1.0 | 0.0 | 1.0 |
| EJANPMFG_4 | 0.0 | 1.0 | 0.0 |
| EJANPMFG_5 | 1.0 | 0.0 | 1.0 |
| EJANPMFG_6 | 0.0 | 1.0 | 0.0 |
| EJANPMFG_7 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EJANPMFG_4 | 790792 | 794473 | False | Siphoviridae | VOG0275, VOG1563, VOG9667 |
| EJANPMFG_4 | 802359 | 826291 | False | Siphoviridae | VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG4705, VOG0641 |
| EJANPMFG_4 | 1963950 | 1981566 | False | Siphoviridae | VOG0054, VOG8537, VOG4574, VOG4705, VOG3390, VOG4626, VOG9712, VOG5042, VOG3389, VOG4910, VOG6163 |
| EJANPMFG_4 | 1993569 | 1999250 | False | Myoviridae | VOG10940, VOG5721, VOG5779 |
| EJANPMFG_4 | 2789091 | 2814387 | False | Myoviridae | VOG0136, VOG0753, VOG7895, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| EJANPMFG_4 | 3527984 | 3550578 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG5615, VOG4705, VOG3390, VOG4599, VOG4605, VOG4545, VOG0725, VOG2621, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG3480, VOG1329, VOG4555, VOG7363, VOG4564, VOG0720, VOG10227, VOG0796 |
Phigaro v2.4.0 (Database: Jan. 2024 release)