safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	8	18.25

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
BFMMGKFP_13	455916	457118	blt	ARO:3003006	blt	100.00	100.00	L32599.1
BFMMGKFP_13	437555	438409	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
BFMMGKFP_12	425607	425924	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
BFMMGKFP_12	425269	425607	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
BFMMGKFP_14	259416	260336	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
BFMMGKFP_14	246082	247521	lmrB	ARO:3002813	lmrB	99.79	100.42	JYFL01000006.1
BFMMGKFP_14	196425	197018	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
BFMMGKFP_18	74756	75925	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
BFMMGKFP_19	47446	49089	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
BFMMGKFP_15	27510	30080	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
BFMMGKFP_13	437555	438409	aadK	Streptomycin	100.00	100.00	M26879
BFMMGKFP_14	259416	260336	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
BFMMGKFP_1	1009699	1011075	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
BFMMGKFP_13	437555	438406	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
BFMMGKFP_14	259419	260336	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
BFMMGKFP_1	1007181	1007699	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
BFMMGKFP_1	1009702	1011075	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
BFMMGKFP_19	47449	49089	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
BFMMGKFP_16	192883	195477	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
BFMMGKFP_1	520716	521858	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
BFMMGKFP_1	522341	523522	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
BFMMGKFP_1	521877	522326	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
BFMMGKFP_16	240372	242135	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
BFMMGKFP_1	114405	115190	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
BFMMGKFP_1	110569	111507	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
BFMMGKFP_1	113183	114379	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
BFMMGKFP_1	111535	113154	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
BFMMGKFP_17	47883	48524	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
BFMMGKFP_1	103413	110549	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.97	100.00	NP_391076

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BFMMGKFP_2	31763	32899	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
BFMMGKFP_4	4356	5225	pdxS	1.12e-151	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
BFMMGKFP_12	738869	739645	CodY	8.25e-125	nutrient-responsive regulator	81.08	100.00	ACJ80679
BFMMGKFP_1	369129	369698	ClpP	6.30e-99	part of proteolytic complex	81.05	97.44	BAB94595
BFMMGKFP_12	276446	276838	spxA1	2.44e-69	redox-responsive transcription factor	83.21	100.00	AAF21893
BFMMGKFP_3	34831	34589	SpoVG	2.75e-38	RNA-binding protein	80.25	79.41	AAT02994
BFMMGKFP_19	1974	2171	CspA	3.38e-32	cold shock protein	90.91	100.00	CAA62903
BFMMGKFP_17	60486	60680	CspD	1.42e-28	cold shock protein	81.54	98.48	CAC99957

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BFMMGKFP_1	0.0	1.0	0.0
BFMMGKFP_2	0.0	1.0	0.0
BFMMGKFP_3	0.0	1.0	0.0
BFMMGKFP_4	0.0	1.0	0.0
BFMMGKFP_5	0.0	1.0	0.0
BFMMGKFP_8	0.0	1.0	0.0
BFMMGKFP_12	0.0	1.0	0.0
BFMMGKFP_13	0.0	1.0	0.0
BFMMGKFP_14	0.0	1.0	0.0
BFMMGKFP_15	0.0	1.0	0.0
BFMMGKFP_16	0.0	1.0	0.0
BFMMGKFP_17	0.0	1.0	0.0
BFMMGKFP_18	0.0	1.0	0.0
BFMMGKFP_19	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
BFMMGKFP_12	369322	395430	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
BFMMGKFP_13	476669	507046	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG6005, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718

Phigaro v2.4.0 (Database: Jan. 2024 release)