Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHDIDNNO_1 | 3227929 | 3230499 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| BHDIDNNO_1 | 3169658 | 3171301 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| BHDIDNNO_1 | 2924589 | 2925182 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BHDIDNNO_1 | 2874086 | 2875519 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| BHDIDNNO_1 | 2861271 | 2862191 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| BHDIDNNO_1 | 1658756 | 1659094 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BHDIDNNO_1 | 1658439 | 1658756 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| BHDIDNNO_1 | 678304 | 679473 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BHDIDNNO_1 | 455953 | 457155 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BHDIDNNO_1 | 437592 | 438446 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BHDIDNNO_1 | 437592 | 438446 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| BHDIDNNO_1 | 2861271 | 2862191 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| BHDIDNNO_1 | 3543545 | 3544921 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHDIDNNO_1 | 437592 | 438443 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| BHDIDNNO_1 | 2861271 | 2862188 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| BHDIDNNO_1 | 3546921 | 3547439 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| BHDIDNNO_1 | 3543545 | 3544918 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| BHDIDNNO_1 | 3169661 | 3171301 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| BHDIDNNO_1 | 985799 | 988393 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHDIDNNO_1 | 2241260 | 2242402 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| BHDIDNNO_1 | 2239596 | 2240777 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| BHDIDNNO_1 | 2240792 | 2241241 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| BHDIDNNO_1 | 1029165 | 1030928 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| BHDIDNNO_1 | 2647927 | 2648712 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| BHDIDNNO_1 | 2651610 | 2652548 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| BHDIDNNO_1 | 2648738 | 2649934 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| BHDIDNNO_1 | 2649963 | 2651582 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| BHDIDNNO_1 | 2652568 | 2659704 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| BHDIDNNO_1 | 878187 | 878828 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHDIDNNO_1 | 3907945 | 3909081 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BHDIDNNO_1 | 3995825 | 3996694 | pdxS | 2.98e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BHDIDNNO_1 | 1345484 | 1344708 | CodY | 3.25e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BHDIDNNO_1 | 2393989 | 2393420 | ClpP | 2.41e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHDIDNNO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BHDIDNNO_1 | 476706 | 507083 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| BHDIDNNO_1 | 1688933 | 1715041 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)