Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCGDMJKN_1 | 3825557 | 3826477 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| MCGDMJKN_1 | 3812231 | 3813664 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| MCGDMJKN_1 | 3762523 | 3763143 | tmrB | ARO:3003059 | tmrB | 97.46 | 104.57 | AL009126.3 |
| MCGDMJKN_1 | 3488513 | 3490156 | vmlR | ARO:3004476 | vmlR | 98.17 | 100.00 | NC_000964.3 |
| MCGDMJKN_1 | 3196223 | 3198793 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| MCGDMJKN_1 | 2733056 | 2733394 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MCGDMJKN_1 | 2732739 | 2733056 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MCGDMJKN_1 | 1748804 | 1749973 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MCGDMJKN_1 | 1527479 | 1528681 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| MCGDMJKN_1 | 1509073 | 1509927 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCGDMJKN_1 | 3825557 | 3826477 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| MCGDMJKN_1 | 1509073 | 1509927 | aadK | Streptomycin | 98.60 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCGDMJKN_1 | 3825560 | 3826477 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| MCGDMJKN_1 | 1509073 | 1509924 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MCGDMJKN_1 | 3488513 | 3490153 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.17 | 100.00 | WP_003234144.1 |
| MCGDMJKN_1 | 29871 | 30389 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| MCGDMJKN_1 | 2042884 | 2045481 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCGDMJKN_1 | 522296 | 523477 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MCGDMJKN_1 | 945545 | 946330 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| MCGDMJKN_1 | 1946641 | 1947282 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| MCGDMJKN_1 | 946356 | 947552 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| MCGDMJKN_1 | 2097962 | 2099725 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| MCGDMJKN_1 | 523492 | 523941 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| MCGDMJKN_1 | 523960 | 525102 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| MCGDMJKN_1 | 950186 | 957322 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391076 |
| MCGDMJKN_1 | 949228 | 950166 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| MCGDMJKN_1 | 947581 | 949200 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCGDMJKN_1 | 3961450 | 3960314 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MCGDMJKN_1 | 4075177 | 4074308 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MCGDMJKN_1 | 2421640 | 2420864 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCGDMJKN_1 | 682737 | 682168 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCGDMJKN_1 | 0.0 | 1.0 | 0.0 |
| MCGDMJKN_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCGDMJKN_1 | 1548243 | 1578279 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| MCGDMJKN_1 | 2763259 | 2789249 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| MCGDMJKN_2 | 7253 | 16618 | False | Unknown | VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561 |
| MCGDMJKN_2 | 37153 | 66080 | False | Siphoviridae | VOG4615, VOG0703, VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221, VOG10500, VOG9096 |
Phigaro v2.4.0 (Database: Jan. 2024 release)