safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
15	10	4	18.47

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
OFLPAGGP_2	4001977	4002897	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
OFLPAGGP_2	3988649	3990082	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
OFLPAGGP_2	3938986	3939579	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
OFLPAGGP_2	3688817	3690460	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
OFLPAGGP_2	3375847	3378417	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
OFLPAGGP_2	2918337	2918675	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
OFLPAGGP_2	2918020	2918337	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
OFLPAGGP_2	1810152	1811321	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
OFLPAGGP_2	1587803	1589005	blt	ARO:3003006	blt	100.00	100.00	L32599.1
OFLPAGGP_2	1569442	1570296	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
OFLPAGGP_1	34575	35225	Streptococcus suis chloramphenicol acetyltransferase	ARO:3004455	Ssui_ACT_CHL	94.91	100.00	AB080798.1
OFLPAGGP_1	33230	34090	TEM-116	ARO:3000979	TEM-116	100.00	100.00	U36911.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
OFLPAGGP_2	1569442	1570296	aadK	Streptomycin	100.00	100.00	M26879
OFLPAGGP_1	33230	34090	blaTEM-116	Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin	100.00	100.00	AY425988
OFLPAGGP_1	34575	35225	cat(pC194)	Chloramphenicol	100.00	100.00	NC_002013
OFLPAGGP_2	4001977	4002897	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
OFLPAGGP_2	116929	118305	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
OFLPAGGP_2	1569442	1570293	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
OFLPAGGP_1	33233	34090	blaTEM-116	broad-spectrum class A beta-lactamase TEM-116	ALLELEX	100.00	100.00	WP_000027050.1
OFLPAGGP_1	34578	35225	catA	type A-9 chloramphenicol O-acetyltransferase	EXACTX	100.00	100.00	WP_001010387.1
OFLPAGGP_2	4001980	4002897	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
OFLPAGGP_2	120305	120823	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
OFLPAGGP_2	116929	118302	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
OFLPAGGP_2	3688817	3690457	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
OFLPAGGP_2	2252046	2254640	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
OFLPAGGP_2	607017	608159	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
OFLPAGGP_2	605353	606534	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
OFLPAGGP_2	606549	606998	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
OFLPAGGP_2	2299534	2301297	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
OFLPAGGP_2	1013682	1014467	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
OFLPAGGP_2	1017365	1018303	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
OFLPAGGP_2	1014493	1015689	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
OFLPAGGP_2	1015718	1017337	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
OFLPAGGP_2	1018323	1025459	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
OFLPAGGP_2	2010032	2010673	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
OFLPAGGP_1	27923	26787	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
OFLPAGGP_2	71306	70437	pdxS	3.05e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
OFLPAGGP_2	2605072	2604296	CodY	3.33e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
OFLPAGGP_2	759746	759177	ClpP	2.47e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
OFLPAGGP_1	32685	32815	ColRNAI	131 / 130	87.02	DQ298019

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
OFLPAGGP_1	0.0	1.0	0.0
OFLPAGGP_2	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
OFLPAGGP_2	1608555	1638932	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
OFLPAGGP_2	2948514	2974622	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667

Phigaro v2.4.0 (Database: Jan. 2024 release)