Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 28 | 35.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KCELLOFB_1 | 43683 | 44522 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| KCELLOFB_1 | 1306835 | 1308094 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.84 | 100.00 | WP_011681445 |
| KCELLOFB_1 | 1081436 | 1082029 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| KCELLOFB_1 | 855227 | 856885 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| KCELLOFB_1 | 1081436 | 1082029 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| KCELLOFB_1 | 1081436 | 1082029 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| KCELLOFB_1 | 1305862 | 1306838 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.49 | 99.90 | WP_011681444 |
| KCELLOFB_1 | 1082029 | 1082898 | rfbA | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011681286 |
| KCELLOFB_1 | 1036593 | 1037383 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.37 | 100.00 | - |
| KCELLOFB_1 | 1304876 | 1305844 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| KCELLOFB_1 | 1116794 | 1117555 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| KCELLOFB_1 | 969859 | 970590 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011681176 |
| KCELLOFB_1 | 969859 | 970590 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| KCELLOFB_1 | 970591 | 972051 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011681177 |
| KCELLOFB_1 | 969859 | 970590 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011227248 |
| KCELLOFB_1 | 1304159 | 1304874 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.18 | 99.86 | WP_011681442 |
| KCELLOFB_1 | 970591 | 972051 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.15 | 100.21 | WP_041827043 |
| KCELLOFB_1 | 968408 | 969148 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011681174 |
| KCELLOFB_1 | 970591 | 972051 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.74 | 100.21 | WP_011226049 |
| KCELLOFB_1 | 968408 | 969148 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| KCELLOFB_1 | 1303043 | 1303813 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.15 | 99.10 | WP_011681440 |
| KCELLOFB_1 | 968415 | 969148 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.00 | 97.87 | WP_011227246 |
| KCELLOFB_1 | 969158 | 969850 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011681175 |
| KCELLOFB_1 | 1308213 | 1309064 | rfbD | 0.0 | Capsule | Immune modulation | 96.95 | 100.00 | WP_014621820 |
| KCELLOFB_1 | 966986 | 968351 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 96.41 | 99.85 | WP_011681173 |
| KCELLOFB_1 | 966984 | 968351 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.49 | 100.00 | WP_011226045 |
| KCELLOFB_1 | 969158 | 969850 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 92.64 | 100.00 | WP_011226047 |
| KCELLOFB_1 | 969158 | 969850 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 92.35 | 100.00 | WP_011227247 |
| KCELLOFB_1 | 615312 | 616615 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| KCELLOFB_1 | 1602920 | 1603930 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| KCELLOFB_1 | 147921 | 149204 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| KCELLOFB_1 | 1079961 | 1081007 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| KCELLOFB_1 | 957157 | 957740 | SPD_RS01775 | 1.08e-146 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KCELLOFB_1 | 1120242 | 1121225 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| KCELLOFB_1 | 714444 | 715517 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| KCELLOFB_1 | 533025 | 533993 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| KCELLOFB_1 | 1478739 | 1481042 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| KCELLOFB_1 | 1193356 | 1194837 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| KCELLOFB_1 | 734965 | 737211 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| KCELLOFB_1 | 63313 | 64305 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| KCELLOFB_1 | 27200 | 28171 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 89.51 | 100.00 | ABF36968 |
| KCELLOFB_1 | 1351569 | 1350418 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| KCELLOFB_1 | 1322132 | 1320144 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| KCELLOFB_1 | 1810635 | 1809157 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| KCELLOFB_1 | 1104046 | 1102316 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.52 | 100.00 | AAK34199 |
| KCELLOFB_1 | 615312 | 616613 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| KCELLOFB_1 | 1795446 | 1793554 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| KCELLOFB_1 | 740136 | 738427 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| KCELLOFB_1 | 1646308 | 1647318 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KCELLOFB_1 | 883206 | 881845 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| KCELLOFB_1 | 1713439 | 1711547 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| KCELLOFB_1 | 600876 | 601766 | bglB | 5.16e-178 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| KCELLOFB_1 | 797817 | 796879 | SP_0676 | 8.08e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| KCELLOFB_1 | 737403 | 738260 | metF | 8.50e-165 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| KCELLOFB_1 | 1008880 | 1008176 | covR | 3.44e-130 | responder protein | 84.68 | 100.00 | AAK71319 |
| KCELLOFB_1 | 1165255 | 1164584 | rr01 | 1.77e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| KCELLOFB_1 | 1008880 | 1008176 | walR | 2.47e-120 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| KCELLOFB_1 | 298289 | 298975 | CovR | 9.89e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| KCELLOFB_1 | 744089 | 744757 | CiaR | 1.99e-117 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| KCELLOFB_1 | 744089 | 744760 | ciaR | 1.95e-115 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| KCELLOFB_1 | 64602 | 64997 | SpxA2 | 3.64e-74 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| KCELLOFB_1 | 149362 | 149862 | RopE (M5005_Spy_1611) | 4.84e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| KCELLOFB_1 | 1478739 | 1481036 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| KCELLOFB_1 | 1698979 | 1697960 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KCELLOFB_2 | 1 | 948 | rep37 | 950 / 948 | 95.47 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KCELLOFB_1 | 0.0 | 1.0 | 0.0 |
| KCELLOFB_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)