Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FABEIBFJ_1 | 2569739 | 2570593 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| FABEIBFJ_1 | 2551026 | 2552228 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| FABEIBFJ_1 | 2351902 | 2353071 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| FABEIBFJ_1 | 1238525 | 1238842 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| FABEIBFJ_1 | 1238187 | 1238525 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FABEIBFJ_1 | 778225 | 780795 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| FABEIBFJ_1 | 464167 | 465810 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| FABEIBFJ_1 | 219457 | 220050 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| FABEIBFJ_1 | 168930 | 170363 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| FABEIBFJ_1 | 156114 | 157034 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FABEIBFJ_1 | 2569741 | 2570593 | aadK | Streptomycin | 98.83 | 99.77 | M26879 |
| FABEIBFJ_1 | 4014646 | 4016022 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.77 | 100.00 | D12567 |
| FABEIBFJ_1 | 156114 | 157034 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FABEIBFJ_1 | 464170 | 465810 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| FABEIBFJ_1 | 4014649 | 4016022 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_063856081.1 |
| FABEIBFJ_1 | 2569742 | 2570593 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| FABEIBFJ_1 | 156114 | 157031 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| FABEIBFJ_1 | 4012125 | 4012643 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| FABEIBFJ_1 | 1916927 | 1919521 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.03 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FABEIBFJ_1 | 3531988 | 3533130 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| FABEIBFJ_1 | 3533613 | 3534794 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| FABEIBFJ_1 | 3533149 | 3533598 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| FABEIBFJ_1 | 2152544 | 2153185 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| FABEIBFJ_1 | 1866544 | 1868307 | dep/capD | 0.0 | Capsule | Immune modulation | 99.60 | 100.00 | NP_389723 |
| FABEIBFJ_1 | 3125617 | 3126402 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| FABEIBFJ_1 | 3121781 | 3122719 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| FABEIBFJ_1 | 3124395 | 3125591 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| FABEIBFJ_1 | 3114627 | 3121761 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 99.97 | NP_391076 |
| FABEIBFJ_1 | 3122747 | 3124366 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FABEIBFJ_1 | 13157 | 14293 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FABEIBFJ_1 | 4064571 | 4065440 | pdxS | 3.12e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FABEIBFJ_1 | 1551966 | 1552742 | CodY | 3.41e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FABEIBFJ_1 | 3380394 | 3380963 | ClpP | 2.53e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FABEIBFJ_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FABEIBFJ_1 | 1182241 | 1208349 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| FABEIBFJ_1 | 2035403 | 2040135 | False | Unknown | VOG0025, VOG7627, VOG0322 |
Phigaro v2.4.0 (Database: Jan. 2024 release)