safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	26	29	38.95

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
FDKOBGMG_1	43550	44389	cbpD	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
FDKOBGMG_1	1138849	1139718	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
FDKOBGMG_1	1138256	1138849	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
FDKOBGMG_1	1358612	1359421	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
FDKOBGMG_1	1138256	1138849	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
FDKOBGMG_1	1002857	1003549	STU_RS14640	Capsule	Immune modulation	99.71	100.00	WP_011226047
FDKOBGMG_1	1138256	1138849	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
FDKOBGMG_1	892281	893939	fbp54	FBPs	Adherence	99.52	100.00	WP_011227179
FDKOBGMG_1	1354551	1356335	STU_RS16230	Capsule	Immune modulation	99.50	100.00	WP_011226325
FDKOBGMG_1	1357407	1358612	STU_RS16240	Capsule	Immune modulation	99.25	100.00	WP_011226326
FDKOBGMG_1	1359405	1360361	STU_RS16250	Capsule	Immune modulation	99.16	100.00	WP_011226328
FDKOBGMG_1	1356332	1357387	STU_RS16235	Capsule	Immune modulation	99.15	100.00	WP_002948022
FDKOBGMG_1	1002857	1003549	STR_RS05255	Capsule	Immune modulation	99.13	100.00	WP_011227247
FDKOBGMG_1	1175041	1175802	srtA	Sortase A	Exoenzyme	99.08	100.00	-
FDKOBGMG_1	1080764	1081554	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
FDKOBGMG_1	1366541	1367392	rfbD	Capsule	Immune modulation	98.71	100.00	WP_014621820
FDKOBGMG_1	984734	986149	STER_RS05250	Capsule	Immune modulation	98.66	100.00	WP_011681165
FDKOBGMG_1	1363202	1364164	STU_RS16260	Capsule	Immune modulation	98.65	95.25	WP_011226330
FDKOBGMG_1	1003558	1004289	STER_RS05315	Capsule	Immune modulation	98.63	100.00	WP_011681176
FDKOBGMG_1	1360358	1361506	STU_RS16255	Capsule	Immune modulation	98.52	100.00	WP_041828290
FDKOBGMG_1	1003558	1004289	STU_RS14645	Capsule	Immune modulation	98.36	100.00	WP_011226048
FDKOBGMG_1	1003558	1004289	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
FDKOBGMG_1	1004290	1005750	STER_RS05320	Capsule	Immune modulation	97.95	100.00	WP_011681177
FDKOBGMG_1	1364167	1365438	STU_RS16265	Capsule	Immune modulation	97.41	99.76	WP_011226331
FDKOBGMG_1	1004290	1005750	STU_RS14650	Capsule	Immune modulation	97.19	100.21	WP_011226049
FDKOBGMG_1	1004290	1005750	STR_RS05265	Capsule	Immune modulation	96.37	100.21	WP_041827043
FDKOBGMG_1	1000665	1002032	STU_RS14630	Capsule	Immune modulation	96.13	100.00	WP_011226045
FDKOBGMG_1	1000667	1002032	STER_RS05300	Capsule	Immune modulation	94.88	99.85	WP_011681173
FDKOBGMG_1	1002089	1002847	STER_RS05305	Capsule	Immune modulation	93.54	100.94	WP_011681174
FDKOBGMG_1	1002089	1002847	STU_RS14635	Capsule	Immune modulation	93.15	100.94	WP_011226046
FDKOBGMG_1	1002094	1002847	STR_RS05250	Capsule	Immune modulation	92.84	99.07	WP_011227246
FDKOBGMG_1	998931	999922	STU_RS14625*	Capsule	Immune modulation	90.79	99.70	-
FDKOBGMG_1	1002857	1003549	STER_RS05310	Capsule	Immune modulation	89.61	100.00	WP_011681175
FDKOBGMG_1	626745	628048	eno	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
FDKOBGMG_1	1665621	1666631	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.46	99.80	WP_002986042
FDKOBGMG_1	160023	161306	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
FDKOBGMG_1	1136781	1137827	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FDKOBGMG_1	1178489	1179472	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
FDKOBGMG_1	735626	736699	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
FDKOBGMG_1	1542793	1545096	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
FDKOBGMG_1	543272	544240	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
FDKOBGMG_1	757539	759785	metE	0.0	methionine biosynthesis enzymes	91.72	100.00	AAK74738
FDKOBGMG_1	1258719	1260200	SPD_0310	0.0	UPF0371 protein SPD_0310	91.50	100.00	ABJ54480
FDKOBGMG_1	72094	73086	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
FDKOBGMG_1	27068	28039	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
FDKOBGMG_1	1428918	1427767	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
FDKOBGMG_1	1380565	1378577	uvrB	0.0	excinuclease ABC, B subunit	88.99	100.00	AE014259_2
FDKOBGMG_1	1162291	1160561	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
FDKOBGMG_1	1885934	1884456	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
FDKOBGMG_1	626745	628046	Eno	0.0	enolase	86.21	99.77	ABP90468
FDKOBGMG_1	1870743	1868851	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
FDKOBGMG_1	762710	761001	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
FDKOBGMG_1	1708623	1709633	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
FDKOBGMG_1	920467	919106	mnmE	0.0	central tRNA-modifying GTPase	81.80	99.56	ABP90420
FDKOBGMG_1	1758940	1757048	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
FDKOBGMG_1	612306	613196	bglB	1.36e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
FDKOBGMG_1	837048	836110	SP_0676	1.78e-166	transcription facot	80.83	97.20	AAK74821
FDKOBGMG_1	759977	760834	metF	8.85e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
FDKOBGMG_1	1042600	1041896	covR	4.48e-131	responder protein	85.53	100.00	AAK71319
FDKOBGMG_1	1230319	1229648	rr01	3.08e-124	response regulator	83.93	99.56	CAB54566
FDKOBGMG_1	766665	767333	CiaR	8.93e-123	two-component response regulator	86.10	99.55	AAK74935
FDKOBGMG_1	1042600	1041896	walR	1.89e-121	DNA-binding response regulator	82.13	100.00	ANC99504
FDKOBGMG_1	766665	767336	ciaR	1.21e-120	two component system response regulator	83.93	99.12	AIK75708
FDKOBGMG_1	318075	318761	CovR	1.05e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
FDKOBGMG_1	73383	73778	SpxA2	3.79e-74	transcriptional regulator	90.91	100.00	ERL21461
FDKOBGMG_1	931827	932228	SpxA1	3.85e-66	transcriptional regulator	80.60	100.00	ERL19273
FDKOBGMG_1	161464	161964	RopE (M5005_Spy_1611)	3.63e-75	probable DNA-directed RNA polymerase subunit delta	82.04	87.43	AAK34603
FDKOBGMG_1	1542793	1545090	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
FDKOBGMG_1	1744483	1743464	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FDKOBGMG_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found