Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBMBJOJE_1 | 2778757 | 2779611 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| BBMBJOJE_1 | 2760096 | 2761298 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| BBMBJOJE_1 | 2534788 | 2535957 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BBMBJOJE_1 | 1428934 | 1429251 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| BBMBJOJE_1 | 1428596 | 1428934 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BBMBJOJE_1 | 966126 | 968696 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BBMBJOJE_1 | 633429 | 635072 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| BBMBJOJE_1 | 392302 | 392895 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BBMBJOJE_1 | 341824 | 343257 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| BBMBJOJE_1 | 329010 | 329930 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BBMBJOJE_1 | 4328553 | 4329929 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| BBMBJOJE_1 | 2778759 | 2779611 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| BBMBJOJE_1 | 329010 | 329930 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BBMBJOJE_1 | 4328556 | 4329929 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| BBMBJOJE_1 | 633432 | 635072 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| BBMBJOJE_1 | 2778760 | 2779611 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| BBMBJOJE_1 | 329010 | 329927 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BBMBJOJE_1 | 4326082 | 4326600 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| BBMBJOJE_1 | 2237196 | 2239793 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BBMBJOJE_1 | 2333342 | 2333983 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| BBMBJOJE_1 | 3399080 | 3400018 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BBMBJOJE_1 | 3843703 | 3844884 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| BBMBJOJE_1 | 3400046 | 3401665 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| BBMBJOJE_1 | 3402916 | 3403701 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| BBMBJOJE_1 | 3842078 | 3843220 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| BBMBJOJE_1 | 3401694 | 3402890 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| BBMBJOJE_1 | 2190265 | 2192028 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| BBMBJOJE_1 | 3391924 | 3399060 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| BBMBJOJE_1 | 3843239 | 3843688 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BBMBJOJE_1 | 132445 | 133581 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BBMBJOJE_1 | 18664 | 19533 | pdxS | 3.24e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BBMBJOJE_1 | 1738796 | 1739572 | CodY | 3.53e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BBMBJOJE_1 | 3702286 | 3702855 | ClpP | 2.62e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BBMBJOJE_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BBMBJOJE_1 | 169358 | 174542 | False | Unknown | VOG3549, VOG1878, VOG0083, VOG1309, VOG1292 |
| BBMBJOJE_1 | 188490 | 213151 | False | Myoviridae | VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG1581, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG0906, VOG7718, VOG4705 |
| BBMBJOJE_1 | 697014 | 720054 | False | Siphoviridae | VOG0796, VOG10227, VOG0720, VOG4564, VOG4555, VOG1329, VOG1329, VOG3480, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG4545, VOG4605, VOG4599, VOG5535, VOG4728, VOG0753, VOG0136, VOG4574 |
| BBMBJOJE_1 | 1371548 | 1399112 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| BBMBJOJE_1 | 1935905 | 1963721 | False | Siphoviridae | VOG4660, VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705, VOG3390, VOG7718, VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867 |
| BBMBJOJE_1 | 2012169 | 2017331 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025 |
| BBMBJOJE_1 | 2712965 | 2745392 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG9315, VOG0796, VOG7060, VOG4673, VOG4567, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
| BBMBJOJE_1 | 2933946 | 2963106 | False | Siphoviridae | VOG3490, VOG4574, VOG0641, VOG5008, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG4841, VOG0198, VOG6517 |
| BBMBJOJE_1 | 3468849 | 3502856 | False | Siphoviridae | VOG4574, VOG0641, VOG5008, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG9091, VOG1884, VOG0025, VOG9600, VOG4566, VOG4552, VOG1638, VOG0787, VOG4556, VOG0181, VOG5353, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)