Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHFOKGKC_1 | 85827 | 86876 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EHFOKGKC_1 | 85827 | 86862 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.61 | 98.67 | KR610408 |
| EHFOKGKC_25 | 356903 | 358279 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.78 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EHFOKGKC_1 | 85827 | 86873 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| EHFOKGKC_25 | 356903 | 358273 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.65 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EHFOKGKC_14 | 275644 | 276825 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| EHFOKGKC_14 | 275180 | 275629 | capC | 2.87e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EHFOKGKC_12 | 31868 | 33004 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| EHFOKGKC_19 | 4502 | 5371 | pdxS | 1.38e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| EHFOKGKC_34 | 186511 | 185735 | CodY | 3.66e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EHFOKGKC_41 | 36910 | 36518 | spxA1 | 6.31e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| EHFOKGKC_1 | 72254 | 72451 | CspA | 8.27e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| EHFOKGKC_23 | 11360 | 11554 | CspD | 3.10e-29 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| EHFOKGKC_23 | 11360 | 11557 | CspB | 6.43e-29 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| EHFOKGKC_1 | 72254 | 72448 | CspR | 1.08e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| EHFOKGKC_2 | 1384 | 2256 | lipA (SAUSA300_0829) | 4.02e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EHFOKGKC_1 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_2 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_3 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_4 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_6 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_9 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_10 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_11 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_12 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_13 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_14 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_15 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_16 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_17 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_18 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_19 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_20 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_21 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_22 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_23 | 1.0 | 0.0 | 1.0 |
| EHFOKGKC_24 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_25 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_26 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_27 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_28 | 1.0 | 0.0 | 1.0 |
| EHFOKGKC_29 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_30 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_32 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_34 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_35 | 1.0 | 0.0 | 1.0 |
| EHFOKGKC_38 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_39 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_41 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_43 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_46 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_48 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_50 | 0.0 | 1.0 | 0.0 |
| EHFOKGKC_52 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EHFOKGKC_43 | 264168 | 280969 | False | Siphoviridae | VOG3390, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389 |
| EHFOKGKC_48 | 161680 | 187967 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)