Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 21 | 34 | 40.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IEGKFECF_2 | 5739 | 6956 | mef(A) | Erythromycin, Azithromycin | 100.00 | 100.00 | U83667 |
| IEGKFECF_2 | 4156 | 5619 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IEGKFECF_2 | 5742 | 6956 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| IEGKFECF_2 | 4159 | 5619 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IEGKFECF_29 | 93784 | 94593 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 95.80 | 100.00 | WP_002948020 |
| IEGKFECF_29 | 96781 | 97632 | rfbD | 0.0 | Capsule | Immune modulation | 94.48 | 100.00 | WP_014621820 |
| IEGKFECF_2 | 80581 | 82041 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.98 | 100.14 | WP_011226049 |
| IEGKFECF_2 | 80581 | 82041 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| IEGKFECF_2 | 80581 | 82041 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| IEGKFECF_2 | 76973 | 78340 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.13 | 100.00 | WP_011226045 |
| IEGKFECF_2 | 76975 | 78340 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 92.97 | 99.85 | WP_011681173 |
| IEGKFECF_29 | 92579 | 93784 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.45 | 100.00 | WP_011226326 |
| IEGKFECF_29 | 95530 | 96678 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 92.43 | 100.00 | WP_041828290 |
| IEGKFECF_22 | 264849 | 265442 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 92.42 | 100.00 | WP_011226149 |
| IEGKFECF_29 | 89760 | 91516 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.38 | 98.38 | WP_011226325 |
| IEGKFECF_22 | 264849 | 265442 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| IEGKFECF_22 | 264849 | 265442 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 92.26 | 100.00 | WP_011226149 |
| IEGKFECF_2 | 79849 | 80580 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| IEGKFECF_29 | 94577 | 95533 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 91.64 | 100.00 | WP_011226328 |
| IEGKFECF_22 | 263980 | 264849 | rfbA | 0.0 | Capsule | Immune modulation | 91.61 | 100.00 | WP_011681286 |
| IEGKFECF_2 | 79849 | 80580 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| IEGKFECF_29 | 91513 | 92559 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.29 | 99.43 | WP_002948022 |
| IEGKFECF_13 | 20568 | 21407 | cbpD | 0.0 | Autolysin | Exoenzyme | 91.07 | 100.00 | WP_011226733 |
| IEGKFECF_2 | 79849 | 80580 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| IEGKFECF_2 | 78398 | 79138 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 90.84 | 99.87 | WP_011681174 |
| IEGKFECF_27 | 43479 | 45133 | fbp54 | 0.0 | FBPs | Adherence | 90.52 | 99.70 | WP_011227179 |
| IEGKFECF_2 | 78398 | 79138 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 89.89 | 99.87 | WP_011226046 |
| IEGKFECF_2 | 78403 | 79138 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 89.55 | 98.00 | WP_011227246 |
| IEGKFECF_22 | 101791 | 103094 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| IEGKFECF_15 | 5795 | 6805 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.36 | 99.80 | WP_002986042 |
| IEGKFECF_2 | 79148 | 79840 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 85.45 | 99.86 | WP_011226047 |
| IEGKFECF_2 | 79148 | 79840 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.16 | 99.86 | WP_011227247 |
| IEGKFECF_2 | 79148 | 79840 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 84.87 | 99.86 | WP_011681175 |
| IEGKFECF_22 | 265645 | 266691 | rfbB | 0.0 | Capsule | Immune modulation | 84.54 | 99.90 | WP_002263087 |
| IEGKFECF_28 | 98284 | 99045 | srtA | 0.0 | Sortase A | Exoenzyme | 84.40 | 99.87 | - |
| IEGKFECF_3 | 1853 | 3136 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IEGKFECF_28 | 101734 | 102717 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.65 | 100.00 | AAK75326 |
| IEGKFECF_28 | 90146 | 89073 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| IEGKFECF_11 | 117855 | 120161 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| IEGKFECF_28 | 71089 | 68843 | metE | 0.0 | methionine biosynthesis enzymes | 93.19 | 100.00 | AAK74738 |
| IEGKFECF_22 | 11325 | 12293 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| IEGKFECF_6 | 61552 | 63033 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.92 | 100.00 | ABJ54480 |
| IEGKFECF_13 | 35788 | 34817 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.36 | 100.00 | ABF36968 |
| IEGKFECF_4 | 39536 | 46084 | PrtA | 0.0 | serine protease | 90.35 | 101.73 | ABJ55268 |
| IEGKFECF_22 | 238598 | 240328 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 90.12 | 100.00 | AAK34199 |
| IEGKFECF_29 | 110096 | 108108 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| IEGKFECF_29 | 138501 | 137350 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| IEGKFECF_5 | 27797 | 26319 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| IEGKFECF_22 | 101791 | 103092 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| IEGKFECF_5 | 12449 | 10557 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.42 | 98.90 | ABP91129 |
| IEGKFECF_28 | 65921 | 67630 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| IEGKFECF_13 | 1903 | 812 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| IEGKFECF_7 | 104599 | 103589 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| IEGKFECF_27 | 27630 | 28991 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| IEGKFECF_22 | 87383 | 88273 | bglB | 4.83e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| IEGKFECF_28 | 68653 | 67796 | metF | 2.47e-170 | methionine biosynthesis enzymes | 88.11 | 99.31 | AAK74739 |
| IEGKFECF_28 | 26035 | 26997 | SP_0676 | 3.75e-170 | transcription facot | 80.69 | 99.69 | AAK74821 |
| IEGKFECF_4 | 2354 | 3025 | rr01 | 1.11e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| IEGKFECF_2 | 126979 | 126275 | covR | 4.27e-125 | responder protein | 85.53 | 100.00 | AAK71319 |
| IEGKFECF_28 | 61254 | 60583 | ciaR | 1.72e-124 | two component system response regulator | 85.71 | 99.12 | AIK75708 |
| IEGKFECF_28 | 61254 | 60586 | CiaR | 4.87e-124 | two-component response regulator | 86.55 | 99.55 | AAK74935 |
| IEGKFECF_3 | 150123 | 150809 | CovR | 4.49e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| IEGKFECF_2 | 126979 | 126275 | walR | 1.10e-118 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| IEGKFECF_13 | 638 | 243 | SpxA2 | 3.90e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| IEGKFECF_27 | 16156 | 15755 | SpxA1 | 1.79e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| IEGKFECF_10 | 43547 | 43960 | GrpE | 3.84e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| IEGKFECF_3 | 173341 | 173628 | spr0479 | 4.35e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| IEGKFECF_9 | 32626 | 32372 | spr1327 | 2.54e-45 | essential gene | 92.94 | 100.00 | AAL00131 |
| IEGKFECF_31 | 6792 | 6529 | spr0175 | 8.47e-44 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| IEGKFECF_31 | 5968 | 5702 | spr0177 | 1.88e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| IEGKFECF_3 | 3303 | 3794 | RopE (M5005_Spy_1611) | 9.93e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| IEGKFECF_11 | 117855 | 120155 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| IEGKFECF_7 | 67909 | 68928 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IEGKFECF_1 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_2 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_3 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_4 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_5 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_6 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_7 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_8 | 1.0 | 0.0 | 1.0 |
| IEGKFECF_9 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_10 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_11 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_12 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_13 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_14 | 1.0 | 0.0 | 1.0 |
| IEGKFECF_15 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_16 | 1.0 | 0.0 | 1.0 |
| IEGKFECF_17 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_18 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_19 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_20 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_21 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_22 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_23 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_24 | 1.0 | 0.0 | 1.0 |
| IEGKFECF_25 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_27 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_28 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_29 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_30 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_31 | 0.0 | 1.0 | 0.0 |
| IEGKFECF_32 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)