Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 20 | 34 | 39.45 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MIPLJNMB_55 | 18501 | 19352 | rfbD | 0.0 | Capsule | Immune modulation | 95.31 | 100.00 | WP_014621820 |
| MIPLJNMB_16 | 24201 | 24932 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 94.81 | 100.00 | WP_011681176 |
| MIPLJNMB_16 | 24201 | 24932 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011226048 |
| MIPLJNMB_16 | 24201 | 24932 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.13 | 100.00 | WP_011227248 |
| MIPLJNMB_55 | 24680 | 26436 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 98.43 | WP_011226325 |
| MIPLJNMB_55 | 20674 | 21630 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 92.48 | 100.00 | WP_011226328 |
| MIPLJNMB_55 | 21614 | 22423 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 92.23 | 99.88 | WP_002948020 |
| MIPLJNMB_55 | 22423 | 23628 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.21 | 100.00 | WP_011226326 |
| MIPLJNMB_55 | 19529 | 20677 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.56 | 99.91 | WP_041828290 |
| MIPLJNMB_55 | 23649 | 24683 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.10 | 99.15 | WP_002948022 |
| MIPLJNMB_31 | 7601 | 8194 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| MIPLJNMB_16 | 24933 | 26393 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 90.92 | 99.86 | WP_011681177 |
| MIPLJNMB_31 | 7601 | 8194 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| MIPLJNMB_31 | 7601 | 8194 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| MIPLJNMB_16 | 24933 | 26393 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.78 | 100.00 | WP_011226049 |
| MIPLJNMB_18 | 37634 | 39292 | fbp54 | 0.0 | FBPs | Adherence | 90.54 | 99.94 | WP_011227179 |
| MIPLJNMB_16 | 24933 | 26393 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| MIPLJNMB_16 | 23500 | 24192 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| MIPLJNMB_16 | 23500 | 24192 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| MIPLJNMB_16 | 23500 | 24192 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| MIPLJNMB_52 | 51817 | 53120 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| MIPLJNMB_20 | 15461 | 16300 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| MIPLJNMB_9 | 13376 | 14389 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| MIPLJNMB_14 | 17622 | 18383 | srtA | 0.0 | Sortase A | Exoenzyme | 83.88 | 99.87 | - |
| MIPLJNMB_31 | 6285 | 7331 | rfbB | 0.0 | Capsule | Immune modulation | 83.49 | 99.90 | WP_002263087 |
| MIPLJNMB_40 | 11924 | 13207 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.53 | 99.61 | WP_000107753 |
| MIPLJNMB_31 | 8194 | 9063 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| MIPLJNMB_16 | 22820 | 23490 | STU_RS14635 | 1.59e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011226046 |
| MIPLJNMB_16 | 22820 | 23490 | STER_RS05305 | 7.39e-147 | Capsule | Immune modulation | 80.83 | 90.28 | WP_011681174 |
| MIPLJNMB_16 | 22888 | 23490 | STR_RS05250 | 7.49e-137 | Capsule | Immune modulation | 81.46 | 80.13 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MIPLJNMB_14 | 21072 | 22055 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| MIPLJNMB_14 | 9488 | 8415 | MntE | 0.0 | manganese homeostasis | 95.81 | 99.72 | ABF32167 |
| MIPLJNMB_23 | 5944 | 3638 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| MIPLJNMB_44 | 13535 | 12567 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| MIPLJNMB_56 | 9699 | 7453 | metE | 0.0 | methionine biosynthesis enzymes | 92.79 | 100.00 | AAK74738 |
| MIPLJNMB_4 | 5696 | 7177 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.71 | 100.00 | ABJ54480 |
| MIPLJNMB_5 | 36313 | 35342 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| MIPLJNMB_7 | 13585 | 7022 | PrtA | 0.0 | serine protease | 90.10 | 101.96 | ABJ55268 |
| MIPLJNMB_21 | 1884 | 154 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| MIPLJNMB_55 | 4869 | 6857 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| MIPLJNMB_8 | 40640 | 41791 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| MIPLJNMB_47 | 10656 | 9178 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| MIPLJNMB_52 | 51817 | 53118 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| MIPLJNMB_24 | 5172 | 7064 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| MIPLJNMB_5 | 1165 | 74 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| MIPLJNMB_56 | 4535 | 6244 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| MIPLJNMB_18 | 23480 | 24841 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| MIPLJNMB_30 | 19361 | 20371 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| MIPLJNMB_52 | 37407 | 38297 | bglB | 1.44e-177 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| MIPLJNMB_56 | 7263 | 6406 | metF | 6.39e-171 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| MIPLJNMB_18 | 123981 | 124919 | SP_0676 | 8.84e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| MIPLJNMB_29 | 23914 | 23057 | mtsA | 6.22e-152 | substrate-binding lipoprotein of manganese transporter | 80.14 | 92.86 | AIK76191 |
| MIPLJNMB_41 | 3304 | 4008 | covR | 1.98e-128 | responder protein | 85.96 | 100.00 | AAK71319 |
| MIPLJNMB_7 | 37629 | 36958 | rr01 | 6.16e-127 | response regulator | 84.38 | 99.56 | CAB54566 |
| MIPLJNMB_17 | 179 | 850 | ciaR | 2.97e-126 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| MIPLJNMB_17 | 179 | 847 | CiaR | 5.50e-126 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| MIPLJNMB_2 | 21351 | 20665 | CovR | 6.85e-121 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| MIPLJNMB_41 | 3304 | 4008 | walR | 1.20e-120 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| MIPLJNMB_18 | 12022 | 11621 | SpxA1 | 1.43e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| MIPLJNMB_6 | 4418 | 4005 | GrpE | 2.91e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| MIPLJNMB_15 | 1907 | 2194 | spr0479 | 3.98e-49 | essential gene | 85.42 | 98.97 | AAK99283 |
| MIPLJNMB_8 | 84530 | 84784 | spr1327 | 2.72e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| MIPLJNMB_24 | 36351 | 36088 | spr0175 | 2.25e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| MIPLJNMB_24 | 35525 | 35259 | spr0177 | 2.79e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| MIPLJNMB_40 | 11757 | 11266 | RopE (M5005_Spy_1611) | 7.08e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| MIPLJNMB_23 | 5944 | 3644 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.97 | 98.08 | MYN69560 |
| MIPLJNMB_30 | 55495 | 54476 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MIPLJNMB_1 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_2 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_3 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_4 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_5 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_6 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_7 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_8 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_9 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_10 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_11 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_12 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_13 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_14 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_15 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_16 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_17 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_18 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_19 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_20 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_21 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_22 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_23 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_24 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_25 | 1.0 | 0.0 | 1.0 |
| MIPLJNMB_26 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_27 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_28 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_29 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_30 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_31 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_32 | 1.0 | 0.0 | 1.0 |
| MIPLJNMB_34 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_35 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_36 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_37 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_38 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_39 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_40 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_41 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_42 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_43 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_44 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_45 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_46 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_47 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_48 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_49 | 1.0 | 0.0 | 1.0 |
| MIPLJNMB_50 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_51 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_52 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_53 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_54 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_55 | 0.0 | 1.0 | 0.0 |
| MIPLJNMB_56 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MIPLJNMB_54 | 65797 | 106915 | False | Siphoviridae | VOG4602, VOG4052, VOG0743, VOG3622, VOG6425, VOG6179, VOG3478, VOG4555, VOG0651, VOG11069, VOG0083, VOG0322, VOG5280, VOG7263, VOG4548, VOG7264, VOG4693, VOG7265, VOG0656, VOG0152, VOG4567, VOG0657, VOG0044, VOG5562, VOG0658, VOG0198, VOG0796, VOG0720, VOG4564, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG3636, VOG4605, VOG4599, VOG0647, VOG0648, VOG0641 |
Phigaro v2.4.0 (Database: Jan. 2024 release)