Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 18 | 32 | 36.72 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EOPNMNAF_2 | 95211 | 96179 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 98.45 | 100.00 | WP_011681443 |
| EOPNMNAF_2 | 94494 | 95209 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 96.09 | 99.86 | WP_011681442 |
| EOPNMNAF_2 | 98522 | 99373 | rfbD | 0.0 | Capsule | Immune modulation | 95.54 | 100.00 | WP_014621820 |
| EOPNMNAF_42 | 17643 | 18374 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 94.54 | 100.00 | WP_011227248 |
| EOPNMNAF_42 | 17643 | 18374 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 93.85 | 100.00 | WP_011226048 |
| EOPNMNAF_42 | 17643 | 18374 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 93.58 | 100.00 | WP_011681176 |
| EOPNMNAF_2 | 97147 | 98400 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 92.51 | 99.52 | WP_011681445 |
| EOPNMNAF_2 | 93378 | 94148 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 91.58 | 99.10 | WP_011681440 |
| EOPNMNAF_42 | 18375 | 19835 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 91.26 | 99.86 | WP_011681177 |
| EOPNMNAF_6 | 20037 | 20630 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.08 | 100.00 | WP_011226149 |
| EOPNMNAF_6 | 20037 | 20630 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| EOPNMNAF_6 | 20037 | 20630 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226149 |
| EOPNMNAF_3 | 42101 | 43759 | fbp54 | 0.0 | FBPs | Adherence | 90.84 | 99.94 | WP_011227179 |
| EOPNMNAF_42 | 18375 | 19835 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 90.65 | 100.00 | WP_011226049 |
| EOPNMNAF_42 | 18375 | 19835 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 89.97 | 100.00 | WP_041827043 |
| EOPNMNAF_42 | 16942 | 17634 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| EOPNMNAF_42 | 16942 | 17634 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| EOPNMNAF_42 | 16942 | 17634 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| EOPNMNAF_18 | 33483 | 34786 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| EOPNMNAF_26 | 5381 | 6220 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| EOPNMNAF_15 | 13375 | 14388 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 85.67 | 99.90 | WP_002986042 |
| EOPNMNAF_3 | 197752 | 198513 | srtA | 0.0 | Sortase A | Exoenzyme | 84.80 | 99.87 | - |
| EOPNMNAF_6 | 20900 | 21946 | rfbB | 0.0 | Capsule | Immune modulation | 83.30 | 99.90 | WP_002263087 |
| EOPNMNAF_8 | 115680 | 116963 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.68 | 99.61 | WP_000107753 |
| EOPNMNAF_6 | 19168 | 20037 | rfbA | 0.0 | Capsule | Immune modulation | 80.28 | 99.77 | WP_002904719 |
| EOPNMNAF_42 | 16262 | 16932 | STU_RS14635 | 2.55e-150 | Capsule | Immune modulation | 81.13 | 90.28 | WP_011226046 |
| EOPNMNAF_42 | 16262 | 16932 | STER_RS05305 | 1.19e-148 | Capsule | Immune modulation | 80.98 | 90.28 | WP_011681174 |
| EOPNMNAF_42 | 16262 | 16932 | STR_RS05250 | 1.20e-138 | Capsule | Immune modulation | 80.09 | 89.20 | WP_011227246 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EOPNMNAF_3 | 201202 | 202185 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| EOPNMNAF_3 | 189614 | 188541 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| EOPNMNAF_33 | 127808 | 125502 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.28 | 99.22 | AAK74619 |
| EOPNMNAF_38 | 13534 | 12566 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| EOPNMNAF_11 | 118222 | 119703 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| EOPNMNAF_3 | 170102 | 167856 | metE | 0.0 | methionine biosynthesis enzymes | 92.66 | 100.00 | AAK74738 |
| EOPNMNAF_24 | 3874 | 4845 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| EOPNMNAF_9 | 1888 | 158 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| EOPNMNAF_4 | 91628 | 90477 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| EOPNMNAF_2 | 114239 | 112251 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.29 | 100.00 | AE014259_2 |
| EOPNMNAF_43 | 7242 | 8720 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| EOPNMNAF_18 | 33483 | 34784 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| EOPNMNAF_43 | 23022 | 24914 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| EOPNMNAF_44 | 12547 | 13638 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| EOPNMNAF_3 | 164938 | 166647 | PGM | 0.0 | phosphoglucomutase | 84.59 | 99.65 | ACH87041 |
| EOPNMNAF_3 | 28340 | 29701 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.89 | 99.56 | ABP90420 |
| EOPNMNAF_22 | 19722 | 20732 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| EOPNMNAF_18 | 19077 | 19967 | bglB | 7.34e-178 | putative phospho--glucosidase | 87.20 | 99.66 | AAK33487 |
| EOPNMNAF_3 | 167666 | 166809 | metF | 6.95e-170 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| EOPNMNAF_3 | 123393 | 124331 | SP_0676 | 1.44e-168 | transcription facot | 81.47 | 97.20 | AAK74821 |
| EOPNMNAF_16 | 32123 | 31419 | covR | 1.51e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| EOPNMNAF_11 | 40438 | 39767 | rr01 | 1.48e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| EOPNMNAF_3 | 160272 | 159601 | ciaR | 2.64e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| EOPNMNAF_3 | 160272 | 159604 | CiaR | 4.83e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| EOPNMNAF_16 | 32123 | 31419 | walR | 3.44e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EOPNMNAF_21 | 36427 | 35741 | CovR | 6.89e-121 | serves to repress GAS virulence factor-encoding genes | 82.10 | 100.00 | ACE75886 |
| EOPNMNAF_3 | 16882 | 16481 | SpxA1 | 1.34e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| EOPNMNAF_10 | 6378 | 5965 | GrpE | 3.06e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| EOPNMNAF_21 | 13226 | 12939 | spr0479 | 2.18e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| EOPNMNAF_4 | 49145 | 48891 | spr1327 | 4.09e-46 | essential gene | 94.12 | 100.00 | AAL00131 |
| EOPNMNAF_43 | 87808 | 87545 | spr0175 | 4.92e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| EOPNMNAF_43 | 86983 | 86717 | spr0177 | 1.09e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| EOPNMNAF_8 | 115513 | 115022 | RopE (M5005_Spy_1611) | 5.73e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| EOPNMNAF_33 | 127808 | 125508 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.36 | 98.08 | MYN69560 |
| EOPNMNAF_22 | 56405 | 55386 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 85.88 | 100.00 | ABJ55451 |
| EOPNMNAF_3 | 56746 | 56207 | pnuC | 1.18e-92 | NAD salvage pathway importer | 82.78 | 66.91 | AAK75931 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EOPNMNAF_1 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_2 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_3 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_4 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_5 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_6 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_7 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_8 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_9 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_10 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_11 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_12 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_13 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_14 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_15 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_16 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_17 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_18 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_19 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_20 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_21 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_22 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_23 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_24 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_25 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_26 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_27 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_28 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_29 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_30 | 1.0 | 0.0 | 1.0 |
| EOPNMNAF_33 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_34 | 1.0 | 0.0 | 1.0 |
| EOPNMNAF_37 | 1.0 | 0.0 | 1.0 |
| EOPNMNAF_38 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_39 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_40 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_42 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_43 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_44 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_45 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_46 | 0.0 | 1.0 | 0.0 |
| EOPNMNAF_47 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)