Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 7 | 16.43 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKLMJFCA_4 | 622424 | 623593 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| BKLMJFCA_2 | 593638 | 593976 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| BKLMJFCA_2 | 593321 | 593638 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BKLMJFCA_4 | 442508 | 443710 | blt | ARO:3003006 | blt | 98.25 | 100.00 | L32599.1 |
| BKLMJFCA_4 | 429253 | 430107 | aadK | ARO:3002627 | aadK | 97.18 | 100.00 | AL009126.1 |
| BKLMJFCA_3 | 355004 | 355924 | mphK | ARO:3004541 | mphK | 96.73 | 100.00 | CP010314.1 |
| BKLMJFCA_3 | 341659 | 343098 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.42 | JYFL01000006.1 |
| BKLMJFCA_3 | 291954 | 292547 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| BKLMJFCA_8 | 263580 | 266150 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| BKLMJFCA_3 | 28945 | 30570 | vmlR | ARO:3004476 | vmlR | 97.97 | 98.90 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BKLMJFCA_4 | 429253 | 430107 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
| BKLMJFCA_3 | 355004 | 355924 | mph(K) | Spiramycin, Telithromycin | 96.85 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BKLMJFCA_3 | 28945 | 30567 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.97 | 98.90 | WP_003234144.1 |
| BKLMJFCA_4 | 429253 | 430104 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| BKLMJFCA_3 | 355007 | 355924 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.73 | 100.00 | WP_003246254.1 |
| BKLMJFCA_4 | 920076 | 922673 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BKLMJFCA_4 | 821632 | 822273 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| BKLMJFCA_1 | 527937 | 529118 | capB | 0.0 | Capsule | Immune modulation | 99.24 | 100.00 | NP_391471 |
| BKLMJFCA_1 | 527473 | 527922 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| BKLMJFCA_1 | 526312 | 527454 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| BKLMJFCA_1 | 113063 | 113848 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.86 | 100.00 | NP_391080 |
| BKLMJFCA_4 | 973287 | 975050 | dep/capD | 0.0 | Capsule | Immune modulation | 98.58 | 100.00 | NP_389723 |
| BKLMJFCA_1 | 109226 | 110164 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| BKLMJFCA_1 | 102070 | 109206 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.86 | 100.00 | NP_391076 |
| BKLMJFCA_1 | 110192 | 111811 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.53 | 100.00 | NP_391078 |
| BKLMJFCA_1 | 111840 | 113036 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.07 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BKLMJFCA_5 | 27947 | 26811 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BKLMJFCA_7 | 4251 | 5120 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BKLMJFCA_2 | 282914 | 282138 | CodY | 8.61e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BKLMJFCA_1 | 371540 | 372109 | ClpP | 6.34e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BKLMJFCA_2 | 785096 | 784704 | spxA1 | 6.77e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| BKLMJFCA_2 | 785096 | 784704 | spx | 4.04e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| BKLMJFCA_6 | 20499 | 20741 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BKLMJFCA_3 | 89945 | 89748 | CspA | 1.90e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BKLMJFCA_1 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_2 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_3 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_4 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_5 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_6 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_7 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_8 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_10 | 0.0 | 1.0 | 0.0 |
| BKLMJFCA_11 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BKLMJFCA_2 | 623230 | 648396 | False | Myoviridae | VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
| BKLMJFCA_2 | 705434 | 732231 | False | Siphoviridae | VOG0275, VOG6116, VOG3477, VOG2703, VOG4707, VOG0641, VOG4643, VOG5535, VOG3390, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG4564, VOG0720, VOG10227, VOG0796 |
| BKLMJFCA_2 | 740635 | 751011 | False | Siphoviridae | VOG1309, VOG4600, VOG4552, VOG8241, VOG9667, VOG9667, VOG3304, VOG0774, VOG4602, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)