Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 7 | 15.78 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFOKHPHF_14 | 123286 | 124455 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
| IFOKHPHF_30 | 21828 | 24398 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IFOKHPHF_26 | 21645 | 21983 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IFOKHPHF_26 | 21328 | 21645 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| IFOKHPHF_13 | 13595 | 15220 | vmlR | ARO:3004476 | vmlR | 97.60 | 98.90 | NC_000964.3 |
| IFOKHPHF_89 | 8239 | 9441 | blt | ARO:3003006 | blt | 98.50 | 100.00 | L32599.1 |
| IFOKHPHF_73 | 4325 | 5080 | aadK | ARO:3002627 | aadK | 96.81 | 88.38 | AL009126.1 |
| IFOKHPHF_58 | 3682 | 4602 | mphK | ARO:3004541 | mphK | 96.08 | 100.00 | CP010314.1 |
| IFOKHPHF_18 | 3387 | 4820 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IFOKHPHF_73 | 4327 | 5081 | aadK | Streptomycin | 98.15 | 88.30 | M26879 |
| IFOKHPHF_58 | 3682 | 4602 | mph(K) | Spiramycin, Telithromycin | 97.18 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IFOKHPHF_13 | 13598 | 15220 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 97.60 | 98.90 | WP_003234144.1 |
| IFOKHPHF_73 | 4328 | 5080 | aadK | aminoglycoside 6-adenylyltransferase AadK | PARTIAL_CONTIG_ENDX | 96.81 | 88.38 | WP_003229862.1 |
| IFOKHPHF_58 | 3685 | 4602 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.08 | 100.00 | WP_003246254.1 |
| IFOKHPHF_91 | 1206 | 3803 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IFOKHPHF_38 | 10055 | 10696 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| IFOKHPHF_17 | 114408 | 114857 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| IFOKHPHF_17 | 114876 | 116018 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| IFOKHPHF_17 | 113212 | 114393 | capB | 0.0 | Capsule | Immune modulation | 99.07 | 100.00 | NP_391471 |
| IFOKHPHF_6 | 43241 | 44026 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| IFOKHPHF_4 | 25758 | 27521 | dep/capD | 0.0 | Capsule | Immune modulation | 98.53 | 100.00 | NP_389723 |
| IFOKHPHF_6 | 46925 | 47863 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.30 | 100.00 | NP_391077 |
| IFOKHPHF_6 | 47883 | 55019 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.19 | 100.00 | NP_391076 |
| IFOKHPHF_6 | 45278 | 46897 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.59 | 100.00 | NP_391078 |
| IFOKHPHF_6 | 44053 | 45249 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.07 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IFOKHPHF_21 | 31660 | 32796 | tufA | 0.0 | elongation factor Tu | 84.70 | 95.69 | AHM69299 |
| IFOKHPHF_55 | 4270 | 5139 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IFOKHPHF_1 | 41406 | 40630 | CodY | 2.30e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IFOKHPHF_22 | 153378 | 153947 | ClpP | 1.14e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| IFOKHPHF_29 | 39529 | 39137 | spxA1 | 5.16e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| IFOKHPHF_29 | 39529 | 39137 | spx | 3.08e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| IFOKHPHF_43 | 34756 | 34514 | SpoVG | 7.46e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| IFOKHPHF_52 | 1185 | 1379 | CspD | 8.62e-29 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| IFOKHPHF_52 | 1185 | 1382 | CspB | 1.79e-28 | cold shock protein | 80.30 | 100.00 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IFOKHPHF_1 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_2 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_3 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_4 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_5 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_6 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_7 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_8 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_9 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_10 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_11 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_12 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_13 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_14 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_15 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_16 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_17 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_18 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_19 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_20 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_21 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_22 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_23 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_25 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_26 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_27 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_28 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_29 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_30 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_31 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_32 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_33 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_34 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_35 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_36 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_37 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_38 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_39 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_40 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_42 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_43 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_44 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_45 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_46 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_47 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_48 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_49 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_50 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_51 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_52 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_53 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_54 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_55 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_56 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_57 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_58 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_59 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_60 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_61 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_62 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_63 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_64 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_65 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_66 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_67 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_68 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_69 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_70 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_71 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_72 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_73 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_74 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_75 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_76 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_77 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_78 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_79 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_80 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_81 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_82 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_83 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_84 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_85 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_86 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_88 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_89 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_90 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_91 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_92 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_93 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_94 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_95 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_96 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_97 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_98 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_99 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_100 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_101 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_102 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_103 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_104 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_107 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_108 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_109 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_110 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_111 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_112 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_115 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_116 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_119 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_120 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_121 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_124 | 1.0 | 0.0 | 1.0 |
| IFOKHPHF_125 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_129 | 0.0 | 1.0 | 0.0 |
| IFOKHPHF_130 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IFOKHPHF_11 | 4370 | 29658 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
Phigaro v2.4.0 (Database: Jan. 2024 release)