Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 0 | 5.10 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MNCOMBKE_1 | 387986 | 388951 | CfxA3 | ARO:3003003 | CfxA3 | 100.00 | 100.00 | AF472622.2 |
| MNCOMBKE_13 | 33902 | 34804 | CepA-29 | ARO:3006224 | CepA-29 | 99.67 | 100.00 | NG_050385.1 |
| MNCOMBKE_39 | 6561 | 7073 | nimE | ARO:3007107 | nimE | 98.24 | 100.00 | CP036548.1 |
| MNCOMBKE_37 | 6051 | 7997 | tet(Q) | ARO:3000191 | tet(Q) | 97.02 | 98.63 | Z21523.1 |
| MNCOMBKE_44 | 169 | 984 | sul2 | ARO:3000412 | sul2 | 100.00 | 100.00 | AY055428.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MNCOMBKE_1 | 387986 | 388951 | cfxA3 | Ampicillin | 100.00 | 100.00 | AF472622 |
| MNCOMBKE_44 | 169 | 984 | sul2 | Sulfamethoxazole | 100.00 | 100.00 | AY034138 |
| MNCOMBKE_13 | 33902 | 34804 | cepA-29 | Unknown Beta-lactam | 99.78 | 100.00 | U05884 |
| MNCOMBKE_39 | 6561 | 7073 | nimE | Metronidazole | 99.42 | 100.00 | AM042593 |
| MNCOMBKE_37 | 6085 | 7997 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 98.17 | 99.33 | L33696 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MNCOMBKE_1 | 387989 | 388951 | cfxA3 | extended-spectrum class A beta-lactamase CfxA3 | EXACTX | 100.00 | 100.00 | WP_004348227.1 |
| MNCOMBKE_44 | 169 | 981 | sul2 | sulfonamide-resistant dihydropteroate synthase Sul2 | EXACTX | 100.00 | 100.00 | WP_001043260.1 |
| MNCOMBKE_13 | 33905 | 34804 | cepA | CepA family extended-spectrum class A beta-lactamase | BLASTX | 99.67 | 100.00 | WP_063865204.1 |
| MNCOMBKE_39 | 6564 | 7073 | nimE | 5-nitroimidazole reductase NimE | BLASTX | 98.24 | 100.00 | WP_005812773.1 |
| MNCOMBKE_37 | 6084 | 7997 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 97.81 | 99.53 | WP_004293868.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MNCOMBKE_23 | 65814 | 67007 | bft-1 | 0.0 | BFT B. fragilis toxin, subtype 1 | Exotoxin | 100.00 | 100.00 | BAA77276 |
| MNCOMBKE_23 | 65814 | 67007 | bft-2 | 0.0 | BFT B. fragilis toxin, subtype 2 | Exotoxin | 94.89 | 100.00 | BAA77277 |
| MNCOMBKE_23 | 65814 | 67007 | bft-3 | 0.0 | BFT B. fragilis toxin, subtype 3 | Exotoxin | 94.47 | 100.00 | BAA77275 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MNCOMBKE_1 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_2 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_3 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_4 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_5 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_6 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_7 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_8 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_9 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_10 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_11 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_12 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_13 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_14 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_15 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_16 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_17 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_18 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_19 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_20 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_21 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_22 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_23 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_24 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_25 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_26 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_27 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_28 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_29 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_30 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_31 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_32 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_33 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_34 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_35 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_36 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_37 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_38 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_39 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_40 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_41 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_42 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_43 | 0.0 | 1.0 | 0.0 |
| MNCOMBKE_44 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_45 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_47 | 1.0 | 0.0 | 1.0 |
| MNCOMBKE_50 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MNCOMBKE_4 | 314027 | 327093 | False | Unknown | VOG4572, |
| MNCOMBKE_28 | 12630 | 20569 | False | Siphoviridae | VOG7238, |
Phigaro v2.4.0 (Database: Jan. 2024 release)