safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	28	27	38.90

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
BFIEHBCH_1	1117965	1118834	rfbA	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681286
BFIEHBCH_1	1117372	1117965	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
BFIEHBCH_1	1333827	1335572	STER_RS07045	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681434
BFIEHBCH_1	1335574	1337274	STER_RS07050	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827753
BFIEHBCH_1	1337292	1338494	STER_RS07055	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681436
BFIEHBCH_1	1338494	1339300	STER_RS07060	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681437
BFIEHBCH_1	1339300	1340241	STER_RS07065	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681438
BFIEHBCH_1	1340238	1341386	STER_RS07070	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681439
BFIEHBCH_1	1341505	1342281	STER_RS07075	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681440
BFIEHBCH_1	1342627	1343343	STER_RS07085	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681442
BFIEHBCH_1	1343344	1344312	STER_RS07090	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681443
BFIEHBCH_1	1345304	1346563	STER_RS07100	0.0	Capsule	Immune modulation	100.00	100.00	WP_011681445
BFIEHBCH_1	1344330	1345307	STER_RS07095	0.0	Capsule	Immune modulation	99.90	100.00	WP_011681444
BFIEHBCH_1	1117372	1117965	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
BFIEHBCH_1	1335574	1337274	STR_RS06890	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827077
BFIEHBCH_1	1117372	1117965	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
BFIEHBCH_1	884098	885756	fbp54	0.0	FBPs	Adherence	99.58	100.00	WP_011227179
BFIEHBCH_1	43628	44467	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
BFIEHBCH_1	1338494	1339300	STR_RS06900	0.0	Capsule	Immune modulation	99.50	100.00	WP_011227429
BFIEHBCH_1	1339300	1340241	STR_RS06905	0.0	Capsule	Immune modulation	99.47	100.00	WP_011227430
BFIEHBCH_1	1337292	1338494	STR_RS06895	0.0	Capsule	Immune modulation	99.42	100.00	WP_011227428
BFIEHBCH_1	1340238	1341386	STR_RS06910	0.0	Capsule	Immune modulation	99.22	100.00	WP_011227431
BFIEHBCH_1	995858	996589	STER_RS05315	0.0	Capsule	Immune modulation	98.50	100.00	WP_011681176
BFIEHBCH_1	995858	996589	STU_RS14645	0.0	Capsule	Immune modulation	98.50	100.00	WP_011226048
BFIEHBCH_1	995858	996589	STR_RS05260	0.0	Capsule	Immune modulation	98.36	100.00	WP_011227248
BFIEHBCH_1	1333827	1335572	STR_RS06885	0.0	Capsule	Immune modulation	98.22	100.00	WP_011227426
BFIEHBCH_1	996590	998050	STER_RS05320	0.0	Capsule	Immune modulation	97.81	100.00	WP_011681177
BFIEHBCH_1	1152737	1153498	srtA	0.0	Sortase A	Exoenzyme	97.77	100.00	-
BFIEHBCH_1	996590	998050	STU_RS14650	0.0	Capsule	Immune modulation	97.06	100.21	WP_011226049
BFIEHBCH_1	994407	995147	STU_RS14635	0.0	Capsule	Immune modulation	97.03	100.00	WP_011226046
BFIEHBCH_1	994407	995147	STER_RS05305	0.0	Capsule	Immune modulation	96.90	100.00	WP_011681174
BFIEHBCH_1	1346682	1347533	rfbD	0.0	Capsule	Immune modulation	96.83	100.00	WP_014621820
BFIEHBCH_1	994412	995147	STR_RS05250	0.0	Capsule	Immune modulation	96.47	98.13	WP_011227246
BFIEHBCH_1	996590	998050	STR_RS05265	0.0	Capsule	Immune modulation	96.23	100.21	WP_041827043
BFIEHBCH_1	992986	994351	STER_RS05300	0.0	Capsule	Immune modulation	95.39	99.85	WP_011681173
BFIEHBCH_1	995157	995849	STR_RS05255	0.0	Capsule	Immune modulation	95.25	99.86	WP_011227247
BFIEHBCH_1	995157	995849	STU_RS14640	0.0	Capsule	Immune modulation	95.25	99.86	WP_011226047
BFIEHBCH_1	992984	994351	STU_RS14630	0.0	Capsule	Immune modulation	94.37	100.00	WP_011226045
BFIEHBCH_1	1062481	1063259	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	93.19	99.37	-
BFIEHBCH_1	995157	995849	STER_RS05310	0.0	Capsule	Immune modulation	89.61	100.00	WP_011681175
BFIEHBCH_1	625192	626495	eno	0.0	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
BFIEHBCH_1	1643323	1644331	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.35	99.31	WP_002986042
BFIEHBCH_1	1338494	1339300	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
BFIEHBCH_1	164139	165422	tig/ropA	0.0	Trigger factor	Stress survival	80.73	99.77	WP_000107753
BFIEHBCH_1	1115897	1116943	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
BFIEHBCH_1	983453	984036	SPD_RS01775	1.10e-146	Capsule	Immune modulation	83.11	96.04	WP_001819836
BFIEHBCH_1	1337757	1338494	SGO_RS05025	0.0	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BFIEHBCH_1	730630	731703	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
BFIEHBCH_1	1156185	1157168	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.21	100.00	AAK75326
BFIEHBCH_1	542950	543918	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
BFIEHBCH_1	1519282	1521585	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
BFIEHBCH_1	1236404	1237885	SPD_0310	0.0	UPF0371 protein SPD_0310	92.71	100.00	ABJ54480
BFIEHBCH_1	752549	754795	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
BFIEHBCH_1	83137	84135	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
BFIEHBCH_1	27144	28115	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
BFIEHBCH_1	1396462	1395311	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
BFIEHBCH_1	1360570	1358582	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
BFIEHBCH_1	1139986	1138256	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
BFIEHBCH_1	1849705	1848227	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
BFIEHBCH_1	625192	626493	Eno	0.0	enolase	86.21	99.77	ABP90468
BFIEHBCH_1	1834967	1833075	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
BFIEHBCH_1	757720	756011	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
BFIEHBCH_1	1686029	1687039	pdh	0.0	pyruvate dehydrogenase	82.79	99.70	ABP91006
BFIEHBCH_1	910733	909372	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
BFIEHBCH_1	1736345	1734453	PepO	0.0	endopeptidase	80.03	100.00	ABF32980
BFIEHBCH_1	610757	611647	bglB	1.34e-178	putative phospho--glucosidase	88.89	99.66	AAK33487
BFIEHBCH_1	830322	829387	SP_0676	7.42e-168	transcription facot	81.73	96.89	AAK74821
BFIEHBCH_1	754987	755844	metF	1.54e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
BFIEHBCH_1	1034848	1034144	covR	4.40e-131	responder protein	85.53	100.00	AAK71319
BFIEHBCH_1	1208300	1207629	rr01	3.02e-124	response regulator	83.93	99.56	CAB54566
BFIEHBCH_1	1034848	1034144	walR	1.85e-121	DNA-binding response regulator	82.13	100.00	ANC99504
BFIEHBCH_1	761674	762342	CiaR	4.53e-119	two-component response regulator	84.75	99.55	AAK74935
BFIEHBCH_1	318418	319104	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
BFIEHBCH_1	761674	762345	ciaR	8.95e-117	two component system response regulator	82.59	99.12	AIK75708
BFIEHBCH_1	84411	84785	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
BFIEHBCH_1	1519282	1521579	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
BFIEHBCH_1	1721891	1720872	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
BFIEHBCH_2	1	951	rep37	954 / 948	87.21	AF177168

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BFIEHBCH_1	0.0	1.0	0.0
BFIEHBCH_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found