Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 0 | 1 | 6.08 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FPIKICAN_12 | 112976 | 114181 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.25 | 100.00 | AF251288.1 |
| FPIKICAN_53 | 12476 | 13336 | ErmF | ARO:3000498 | ErmF | 98.50 | 107.52 | M17124.1 |
| FPIKICAN_53 | 10264 | 12189 | tet(Q) | ARO:3000191 | tet(Q) | 89.08 | 97.56 | Z21523.1 |
| FPIKICAN_59 | 705 | 2630 | tet(Q) | ARO:3000191 | tet(Q) | 96.41 | 97.56 | Z21523.1 |
| FPIKICAN_79 | 59 | 874 | sul2 | ARO:3000412 | sul2 | 100.00 | 100.00 | AY055428.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FPIKICAN_79 | 59 | 874 | sul2 | Sulfamethoxazole | 100.00 | 100.00 | AY034138 |
| FPIKICAN_59 | 705 | 2630 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| FPIKICAN_53 | 12476 | 13276 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.50 | 100.00 | M17808 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FPIKICAN_79 | 62 | 874 | sul2 | sulfonamide-resistant dihydropteroate synthase Sul2 | EXACTX | 100.00 | 100.00 | WP_001043260.1 |
| FPIKICAN_59 | 708 | 2630 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| FPIKICAN_12 | 114206 | 114715 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | BLASTX | 99.41 | 100.00 | WP_004308783.1 |
| FPIKICAN_12 | 112976 | 114178 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.25 | 100.00 | WP_063853729.1 |
| FPIKICAN_53 | 12479 | 13276 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 98.87 | 100.00 | WP_002682030.1 |
| FPIKICAN_53 | 10267 | 12189 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 89.55 | 100.00 | WP_063856407.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FPIKICAN_6 | 42438 | 44018 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 80.64 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FPIKICAN_71 | 2147 | 2727 | repUS2 | 584 / 681 | 80.48 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FPIKICAN_1 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_2 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_3 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_4 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_5 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_6 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_7 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_8 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_9 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_10 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_11 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_12 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_13 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_14 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_15 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_16 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_17 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_18 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_19 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_20 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_21 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_22 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_23 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_24 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_25 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_26 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_27 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_28 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_29 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_30 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_31 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_32 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_33 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_34 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_35 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_36 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_37 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_38 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_39 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_40 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_41 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_42 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_43 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_44 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_45 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_46 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_47 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_48 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_49 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_50 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_51 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_52 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_53 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_54 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_55 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_56 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_57 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_58 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_59 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_60 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_61 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_62 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_63 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_64 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_65 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_66 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_67 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_68 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_69 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_70 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_71 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_72 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_73 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_74 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_75 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_76 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_77 | 1.0 | 0.0 | 1.0 |
| FPIKICAN_78 | 0.0 | 1.0 | 0.0 |
| FPIKICAN_83 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FPIKICAN_1 | 99785 | 111776 | False | Unknown | VOG0572, |
Phigaro v2.4.0 (Database: Jan. 2024 release)